Molecular chaperones, such as the small heat shock proteins (sHsps), maintain normal cellular function by controlling protein homeostasis in stress conditions. However, sHsps are not only activated response to environmental insults, but also exert developmental and tissue-specific functions that much less known. Here, we show during development Drosophila sHsp CryAB [L(2)efl] is specifically expressed larval body wall muscles accumulates at level of Z-bands around myonuclei. features a...

Correct diversification of cell types during development ensures the formation functional organs. The evolutionarily conserved homeobox genes from ladybird/Lbx family were found to act as identity in a number embryonic tissues. A prior genetic analysis showed that Drosophila muscle and heart ladybird is required for specification subset muscular cardiac precursors. To learn how exert their functions we performed heart-targeted genome-wide transcriptional profiling chromatin...

A limited number of signalling pathways are involved in the specification cell fate during development all animals. Several these were originally identified Drosophila. To clarify their roles, and possible cross-talk, we have built a logical model for nine key recurrently used metazoan development. In each case, considered associated ligands, receptors, signal transducers, modulators, transcription factors reported literature. Implemented using modelling software GINsim, resulting models...

Mapping the regulatory modules to which transcription factors bind in vivo is a key step toward understanding of global gene expression programs. We have developed chromatin immunoprecipitation (ChIP)-chip strategy for identifying factor-specific regions acting vivo. This method, called ChIP-enriched silico targets (ChEST) approach, combines cross-linked protein-DNA complexes (X-ChIP) with prediction and generation computed DNA microarrays. report use ChEST Drosophila identify several...

Measuring levels of mRNAs in the process translation individual cells provides information on proteins involved cellular functions at a given point time. The protocol dubbed Translating Ribosome Affinity Purification (TRAP) is able to capture this mRNA cell-type-specific manner. Based affinity purification polysomes carrying tagged ribosomal subunit, TRAP can be applied translatome analyses cells, making it possible compare cell types during course developmental processes or track disease...

Given the complexity of developmental networks, it is often difficult to predict effect genetic perturbations, even within coding genes. Regulatory factors generally have pleiotropic effects, exhibit partially redundant roles, and regulate highly interconnected pathways with ample cross-talk. Here, we delineate a logical model encompassing 48 components 82 regulatory interactions involved in mesoderm specification during Drosophila development, thereby providing formal integration all...

Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into disorders remains poorly understood. Here we simulated CELF1 the Drosophila heart performed TU-tagging-based RNAseq of cardiac cells. We detected deregulations several genes controlling cellular calcium levels, including increased expression straightjacket/α2δ3, which...

Developmental patterning and tissue formation are regulated through complex gene regulatory networks (GRNs) driven the action of transcription factors (TFs) converging on enhancer elements. Here, as a point entry to dissect poorly defined GRN underlying cardiomyocyte differentiation, we apply an integrated approach identify active enhancers TFs involved in Drosophila heart development. The consists 104 cardiomyocytes, representing less than 0.5% all cells embryo. By modifying BiTS-ChIP for...

Abstract A combinatorial code of identity transcription factors (iTFs) specifies the diversity muscle types in Drosophila . We previously showed that two iTFs, Lms and Ap, play critical role a subset larval body wall muscles, lateral transverse (LT) muscles. Intriguingly, small portion ap lms mutants displays an increased number LT phenotype recalls pathological split fibers human. However, genes acting downstream Ap to prevent these aberrant feature are not known. Here, we applied cell type...

Despites several decades of studies on the neuromuscular system, relationship between muscle stem cells and motor neurons remains elusive. Using Drosophila model, we provide evidences that adult precursors (AMPs), cells, interact with axons during embryogenesis. AMPs not only hold capacity to attract navigating intersegmental (ISN) segmental a (SNa) nerve branches, but are also mandatory innervation muscles in lateral field. This so far ignored role involves their filopodia-based...

Abstract RNA Polymerase II (RNAP II) pausing is essential to precisely control gene expression and critical for development of metazoans. Here, we show that the m 6 A modification regulates promoter-proximal RNAP pausing. The methyltransferase complex (MTC), with nuclear reader Ythdc1, are recruited promoters. Depleting MTC leads a decrease in pause release Ser2P occupancy on body, affects nascent transcription. Tethering Mettl3 heterologous promoter sufficient increase release, an effect...

Formation of functional organs requires cell–cell communication between different cell lineages and failure in this can result severe developmental defects. Hundreds possible interacting pairs proteins are known, but identifying the partners that ensure a specific interaction 2 given types remains challenging. Here, we use Drosophila leg model our type-specific transcriptomic data sets to uncover molecular mediators tendon muscle precursors. Through analysis gene expression signatures...

ABSTRACT Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a complex toxic CTG repeat disorder involving misbalance between two RNA- binding factors, MBNL1 and CELF1. How this pathogenic DM1 condition translates into cardiac disorders remains poorly understood. Here, we simulated CELF1 the Drosophila heart identified associated gene deregulations using TU-tagging based transcriptional profiling of cells. We detected several genes controlling cellular...

Abstract The formation of the cardiac tube is a remarkable example complex morphogenetic process conserved from invertebrates to humans. It involves coordinated collective migration contralateral rows cells. molecular processes underlying specification cardioblasts prior are well established and significant advances have been made in understanding lumen formation. However, mechanisms cells remain elusive. Here we identified CAP MSP-300 as novel actors involved cardioblast migration. They...

The formation of the cardiac tube is a remarkable example complex morphogenetic processes conserved from invertebrates to humans. It involves coordinated collective migration contralateral rows cells. molecular underlying specification cardioblasts (CBs) prior are well established and significant advances have been made in understanding process lumen formation. However, mechanisms cells remain elusive. Here, we identified CAP MSP300 as novel actors involved during CB migration. They both...

Measuring levels of mRNAs in the process translation individual cells provides information on proteins involved cellular functions at a given point time. The protocol dubbed Translating Ribosome Affinity Purification (TRAP) is able to capture this mRNA cell-type-specific manner. Based affinity purification polysomes carrying tagged ribosomal subunit, TRAP can be applied translatome analyses cells, making it possible compare cell types during course developmental processes or track disease...

Formation of functional organs requires cell-cell communication between different cell lineages, and failure in this can result severe developmental defects. Hundreds possible interacting pairs proteins are known, but identifying the partners that ensure a specific interaction two given types remains challenging. Here, we use Drosophila leg model our type-specific transcriptomic datasets to uncover molecular mediators tendon muscle precursors. Through analysis gene expression signatures...