A5100452978- Parasitic Diseases Research and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Turtle Biology and Conservation
- Parasites and Host Interactions
- Animal Behavior and Reproduction
- Amphibian and Reptile Biology
- Reproductive biology and impacts on aquatic species
- Parasite Biology and Host Interactions
- Viral gastroenteritis research and epidemiology
- Antifungal resistance and susceptibility
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Mosquito-borne diseases and control
- Complement system in diseases
- Physiological and biochemical adaptations
- Hepatitis Viruses Studies and Epidemiology
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Virology and Viral Diseases
- Genetic diversity and population structure
- SARS-CoV-2 and COVID-19 Research
- Orthopedic Surgery and Rehabilitation
- Urban and spatial planning
- Gut microbiota and health
- Protist diversity and phylogeny
Capital Medical University
2016-2025
Beijing Children’s Hospital
2025
Ministry of Agriculture and Rural Affairs
2019-2024
Chinese Academy of Fishery Sciences
2009-2024
Shanghai Ocean University
2009-2024
Beijing YouAn Hospital
2024
Nanjing Agricultural University
2022-2023
Civil Aviation Flight University of China
2022
Nanchang University
2019-2020
Zhengzhou Children's Hospital
2017
Trichinellosis is a worldwide zoonosis and remains serious public health problem. Interrupting parasite transmission via vaccination of livestocks with potent vaccine practical approach to prevent human Trichinellosis. Our previous studies have identified that paramyosin Trichinella spiralis (Ts-Pmy) good candidate against In this study, novel multi-epitope (MEP) was constructed by using four CD4+ T cell epitopes (P2, P3, P4, P5) one B epitope (YX1) from Ts-Pmy expressed as soluble...
The hygiene hypothesis proposes that decreased exposure to infectious agents in developed countries may contribute the development of allergic and autoimmune diseases. Trichinella spiralis, a parasitic roundworm, causes trichinellosis, also known as trichinosis, humans. T. spiralis had many hosts, almost any mammal could become infected. Adult worms lived small intestine, while larvae muscle cells same mammal. was significant public health threat because it cause severe illness even death...
Paramyosin is a thick myofibrillar protein found exclusively in invertebrates. Evidence suggested that paramyosin from helminths serves not only as structural but also an immunomodulatory agent. We previously reported recombinant Trichinella spiralis (Ts-Pmy) elicited partial protective immunity mice. In this study, the ability of Ts-Pmy to bind host complement components and protect against attack was investigated.In transcriptional expression levels were determined T. newborn larva (NBL),...
Trichinellosis is one of the most important food-borne parasitic zoonoses throughout world. Because infected pigs are major source human infections, and China becoming largest international producer pork, development a transmission-blocking vaccine to prevent swine from being urgently needed for trichinellosis control in China. Our previous studies have demonstrated that specific Trichinella spiralis paramyosin (Ts-Pmy) Ts-87 antigen could provide protective immunity against T. infection...
Trichinella spiralis maintains chronic infections within its host, involving a variety of immunomodulatory properties, the mechanisms which have not been completely elucidated. In this study, we found that T. infection induced strong regulatory T cell responses through parasite excretory-secretory (ES) products, characterized by increase CD4+CD25+Foxp3+ and CD4+CD25-Foxp3+ Treg cells accompanied high levels IL-10 TGF-β. adult worm products (AES) muscle larvae (MES) were both able to activate...
As a multicellular parasitic nematode, Trichinella spiralis regulates host immune responses by producing variety of immunomodulatory molecules to escape from attack, but the mechanisms underlying evasion are not well understood. Here, we identified that T. calreticulin (Ts-CRT), Ca2+ binding protein, facilitated interacting with first component human classical complement pathway, C1q. In present study, Ts-CRT was found be expressed on surface different developmental stages as in secreted...
Introduction: Bone morphogenetic proteins (BMPs) play a crucial role in bone formation and differentiation. Recent RNA-Seq results suggest that BMPs may be involved the sex differentiation of P. sinensis, yet more relevant studies about sinensis are lacking. Methods: Herein, we identified BMP gene family members, analyzed phylogeny, collinear relationship, scaffold localization, structures, protein transcription factors dimorphic expression by using bioinformatic methods based on genomic...
BACKGROUND: Hypoxia-induced pulmonary hypertension (HPH) is a T helper 17 cell response-driven disease, and PD-1 (programmed death 1)/PD-L1 death-ligand 1) inhibitor–associated has been reported recently. This study designed to explore whether the PD-1/PD-L1 pathway participates in HPH via regulating endothelial dysfunction response. METHODS: Lung tissue samples were obtained from eligible patients. Western blotting, immunohistochemistry, immunofluorescence techniques used assess protein...
Objective: To analyze the colonization rate and molecular types of Staphylococcus aureus (SA) on tracheotomy wounds neonates at early postoperative stage in neonatal intensive care unit (NICU). Methods: This was a case series study. Patients who were admitted underwent NICU Beijing Children's Hospital, Capital Medical University from January 1st 2020 to December 31st 2023 enrolled. Swabs skin around incision or nasal mucosa collected cultured 24, 72 168 h after operation. Coagulase test...
Dendritic cells (DCs) are antigen-presenting that regulate T cell responses for many infectious diseases. The tissue-dwelling nematode Trichinella spiralis expresses paramyosin (TsPmy) not only as a structural protein but also an immunomodulator to alleviate complement attack by binding some host components. Whether TsPmy is involved in other immunomodulatory pathway and how interacts with DCs still unknown. Mouse bone marrow-derived were incubated recombinant (rTsPmy) activation. Maturation...
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 infection in humans can cause acute haemorrhagic colitis and severe haemolytic uraemic syndrome. The role of enterohaemolysin (Ehx) the pathogenesis O157:H7-mediated disease remains undefined. Recent studies have revealed importance inflammatory response humans. We previously reported that Ehx markedly induced interleukin-1β (IL-1β) production human macrophages. Here, we investigated disparity Ehx-induced IL-1β between mouse macrophages...
Trichinellosis is an important foodborne zoonosis that distributed worldwide. Trichinella spiralis may evade host complement-mediated attack by expressing complement inhibitory proteins, such as paramyosin (Pmy). Previous studies have shown (Ts-Pmy) able to bind the human component C9 inhibit activation and protect parasite from attack. Further determination of complement-binding domain on Ts-pmy will enable us better understand Ts-Pmy's biofunction in immune evasion provide feasible...
Multiepitope peptide vaccine has some advantages over traditional recombinant protein due to its easy and fast production possible inclusion of multiple protective epitopes pathogens. However, it is usually poorly immunogenic needs conjugate a large carrier protein. Peptides conjugated central lysine core form antigen peptides (MAPs) will increase the immunogenicity vaccine. In this study, we constructed MAP consisting CD4 + T cell B paramyosin (Pmy) Trichinella spiralis ( Ts -Pmy), which...
Abstract Knowledge of sex determination has important implications in physiology, ecology and genetics, but the evolutionary mechanisms systems turtles have not been fully elucidated, due to a lack reference genomes. Here, we generate high-quality genome assembly Asian yellow pond turtle ( Mauremys mutica ) using continuous long-read (PacBio platform), Illumina, high-throughput chromatin conformation capture (Hi-C) technologies. The M . haplotype size 2.23 Gb with contig N50 8.53 Mb scaffold...
Male and female Chinese soft-shelled turtles (Pelodiscus sinensis) have sex-dimorphic growth patterns, males higher commercial value because of their larger size thicker calipash. Thus, developing sex-specific markers is beneficial to studies on all-male breeding in P. sinensis. Here, we developed an accurate efficient workflow for the screening sequences with ZW or XY sex determination systems. Based this workflow, male sinensis reference genomes 2.23 Gb 2.26 were obtained using de novo...
Helminths produce calreticulin (CRT) to immunomodulate the host immune system as a survival strategy. However, structure of helminth-derived CRT and structural basis evasion process remains unclarified. Previous study found that tissue-dwelling helminth Trichinella spiralis produces (TsCRT), which binds C1q inhibit activation complement classical pathway. Here, we used x-ray crystallography resolve truncated TsCRT (TsCRT Δ ), first CRT. was observed share same binding region on with IgG...
In aquaculture, the Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important species with remarkable gender dimorphism in its growth patterns. However, underlying molecular mechanisms of this phenomenon have not been elucidated well. Here, we conducted a whole-transcriptome analysis female and male gonads P. sinensis. Overall, 7833 DE mRNAs, 619 lncRNAs, 231 circRNAs, 520 miRNAs were identified. Some "star genes" associated sex differentiation containing dmrt1, sox9,...
Trichinellosis is a widespread zoonosis primarily caused by Trichinella spiralis . Mucosal immunity crucial for preventing infection. In our previous study, DNA vaccine with the antigen Ts87 delivered an attenuated Salmonella typhimurium elicited partial protection against infection in mice. current to elicit more robust immune response and develop potent vaccination strategy trichinellosis, heterologous prime-boost regimen was used mice protective efficacy evaluated compared homologous or...
The Asian giant softshell turtle, Pelochelys cantorii (Trionychidae), is endangered and was designated as a First Grade Protected Animal in China 1989 listed Endangered on the International Union for Conservation of Nature Red List 2000; species has suffered gradual reduction its range, total population decreased, need to protect this been ignored. We investigated 13 nature reserves with P. main object protection 5 captive-breeding centers; we also present results from questionnaire sent one...
Helminth infection modulates host regulatory immune responses to maintain homeostasis. Our previous study identified Trichinella spiralis paramyosin ( Ts Pmy) as a major immunomodulatory protein with the ability induce T cells (Tregs). However, whether Pmy regulates gut Tregs and contributes intestinal homeostasis remains unclear. Here we investigated therapeutic effect of recombinant (r on experimental colitis in mice, elucidated roles mechanisms colonic induced by r ameliorating colitis....
Trichinellosis is a serious food-borne parasitic zoonosis worldwide. Different host macrophage subsets play various roles during helminth infection; however, the dynamic changes in following Trichinella spiralis infection have not been reported. Here, flow cytometry and immunofluorescence were used to assess activation mesenteric lymph nodes (MLN), spleen, intestine, muscle from T. spiralis-infected mice at 1, 5, 15, 30 days post (dpi). Macrophages MLN, spleen tended be activated M1-type 1 5...