A5008574730- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Protein purification and stability
- Identification and Quantification in Food
- Forensic Toxicology and Drug Analysis
- Genomics and Phylogenetic Studies
- Edible Oils Quality and Analysis
- Microbial Metabolites in Food Biotechnology
- Spectroscopy and Chemometric Analyses
- Enzyme Structure and Function
- Essential Oils and Antimicrobial Activity
- Bee Products Chemical Analysis
- Isotope Analysis in Ecology
- Mycotoxins in Agriculture and Food
- Diatoms and Algae Research
- Advanced Chemical Sensor Technologies
- Pesticide Residue Analysis and Safety
- Viral Infectious Diseases and Gene Expression in Insects
- Marine Biology and Environmental Chemistry
- Biosimilars and Bioanalytical Methods
- Phytochemistry and Biological Activities
- Celiac Disease Research and Management
- Tea Polyphenols and Effects
Bruker (Germany)
2011-2024
Fahrenheit (Germany)
2010-2023
Bruker (Canada)
2023
Knorr-Bremse (Germany)
2015-2022
Bruker (United States)
2013
Saarland University
2009
University of Salzburg
2009
Leiden University Medical Center
2007
The European Medicines Agency received recently the first marketing authorization application for a biosimilar monoclonal antibody (mAb) and adopted final guidelines on mAbs Fc-fusion proteins. agency requires high similarity between reference products approval. Specifically, amino acid sequences must be identical. glycosylation pattern of is also often considered to very important quality attribute due its strong effect quality, safety, immunogenicity, pharmacokinetics potency. Here, we...
Hybrid quadrupole time-of-flight (QTOF) mass spectrometry is one of the two major principles used in proteomics. Although based on simple fundamentals, it has over last decades greatly evolved terms achievable resolution, accuracy, and dynamic range. The Bruker impact platform QTOF instruments takes advantage these developments here we develop evaluate II for shotgun proteomics applications. Adaption our heated liquid chromatography system achieved very narrow peptide elution peaks. equipped...
In order to understand the distribution of main secondary metabolites found in Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well virgin olive oil, oil obtained from stoned dehydrated fruits seed oil) coming a Picudo cv. tree were analyzed. All experimental conditions selected so assure maximum coverage metabolome under study within single run. The use LC GC with high resolution MS (through ionization sources, ESI APCI) annotation strategies...
Gas Chromatography (GC)-Mass Spectrometry (MS) with Atmospheric Pressure (AP) interface was introduced more than 30 years ago but never became a mainstream technique, mainly because of technical difficulties and cost instrumentation. A recently multipurpose AP source created the opportunity to reconsider importance ionization for GC. Here, we present an analytical evaluation GC/APCI-MS showing benefits soft atmospheric pressure chemical GC in combination Time Flight (TOF) mass analyzer....
Looking for a strategy to authenticate the declared origin of commercial extra virgin olive oils (EVOOs), 126 samples from six different Mediterranean geographical indications (GIs) are analyzed by means two platforms [LC‐ESI‐QTOF MS (in positive and negative polarity) GC‐APCI‐QTOF mode)] combined chemometrics. The sample treatment chromatographic/detection conditions both platforms) chosen enable comprehensive characterization complete minor fraction within single run. Noticeable...
De novo sequencing of peptides using tandem MS is difficult due to missing fragment ions in the spectra commonly obtained after CID peptide precursor ions. Complementing with an ion-trap mass spectrometer upon electron transfer dissociation (ETD) significantly increases sequence coverage diagnostic In de algorithm CompNovo presented here, a divide-and-conquer approach was combined efficient decomposition exploit complementary information contained and ETD spectra. After optimizing parameters...
Liquid chromatography time-of-flight mass spectrometry (LC-TOFMS) is applied increasingly to various fields of small molecule analysis. The moderate resolving power (RP) standard TOFMS instruments poses a risk false negative results when complex biological matrices are be analyzed. In this study, the performance high instrument (maXis by Bruker Daltonik, Bremen, Germany) was evaluated for drug By flow injection analysis critical mixtures, including total 17 compounds with nominal masses...
Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in high market price. This fact indicates the need protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied identify botanical origin markers of Greek honey. Blossom samples (n = 62) unifloral fir 10), oak 24), pine 39) thyme 34) honeys analyzed using an...
Trapped ion mobility spectrometry (TIMS) is a promising technique for the separation of isomers based on their mobility. In present work, TIMS coupled to liquid chromatography (LC) and high-resolution mass (HRMS) was applied as comprehensive analytical platform address authenticity challenges, focusing extra virgin olive oil (EVOO). Isomers detected in EVOO's phenolic fraction, classified into secoiridoids group, were successfully separated. Thanks parallel accumulation serial fragmentation...
Shotgun proteome analysis of the myxobacterial model strain for secondary metabolite biosynthesis Sorangium cellulosum was performed employing off-line two-dimensional high-pH reversed-phase HPLC x low-pH ion-pair and dual tandem mass spectrometry with collision-induced dissociation (CID) electron transfer (ETD) as complementary fragmentation techniques. Peptide identification using database searching optimized ETD fragment spectra to obtain maximum number identifications at equivalent false...
We developed a method for comprehensive urine drug screening by applying dilute-and-shoot extraction and vacuum-insulated probe-heated electrospray ionization with ultra-high performance liquid chromatography high-resolution quadrupole time-of-flight mass spectrometry (DS-UHPLC-VIP-HESI-QTOFMS). The involved five-fold post-hydrolysis dilution of samples on C18 UHPLC column prior to QTOFMS analysis. recently introduced VIP-HESI ion source was chosen due its enhanced efficiency compatibility...