A5086414906- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Advanced Proteomics Techniques and Applications
- Analytical chemistry methods development
- Ion-surface interactions and analysis
- Spectroscopy and Chemometric Analyses
- Metabolomics and Mass Spectrometry Studies
- Phytochemicals and Antioxidant Activities
- Diamond and Carbon-based Materials Research
- Essential Oils and Antimicrobial Activity
- Advanced Chemical Sensor Technologies
- Identification and Quantification in Food
- Microfluidic and Capillary Electrophoresis Applications
- Mycotoxins in Agriculture and Food
- Spectroscopy Techniques in Biomedical and Chemical Research
- Biochemical Analysis and Sensing Techniques
- Inorganic and Organometallic Chemistry
- Alcohol Consumption and Health Effects
- Glycosylation and Glycoproteins Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Electrochemical Analysis and Applications
- Adsorption, diffusion, and thermodynamic properties of materials
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Protein purification and stability
Universität Innsbruck
2016-2025
Czech Academy of Sciences, Institute of Analytical Chemistry
2021-2024
Institute for Biomedicine
2020
Management Center Innsbruck
2014
Peking University
2013
Bahauddin Zakariya University
2008
Sony Digital Audio Disc Corporation (Austria)
2008
University Hospital Ulm
2008
Biocrates Life Sciences (Austria)
2005
Universität der Bundeswehr München
2002
Sample pretreatment is the most important procedure to remove matrix for interfacing with mass spectrometry (MS). Additionally, samples low concentration, process of preconcentration required before MS analysis. We have newly developed a solid-phase extraction stationary phase based on C60-fullerene covalently bound silica purification biomolecules different characteristics. Silica particles porosity are modified aminopropyl linker and then C60-fullerenoacetic acid or C60-epoxyfullerenes....
Abstract The potential of an organic monolith with incorporated titanium dioxide (TiO 2 ) and zirconium (ZrO nanoparticles was evaluated for the selective enrichment phosphorylated peptides from tryptic digests. A pipette tip fitted a based on divinylbenzene (DVB) highly porous structure, which allows sample to pass through monolithic bed. phosphopeptides enhanced by increasing pipetting cycles during preparation higher recovery could be achieved adequate buffer systems. complete automated...
Elderberry (Sambucus nigra L., fructus) is a very potent herbal drug, deriving from traditional European medicine (TEM). Ripe elderberries are rich in anthocyanins, flavonols, flavonol esters, glycosides, lectins, essential oils, unsaturated fatty acids and vitamins. Nevertheless, unripe elderflower fruits contain certain amount of sambunigrin, toxic cyanogenic glycoside, whose concentration decreases the ripening process. Therefore, quality assurance must be carried out. The standard method...
A nanostructured diamond-like carbon (DLC) coated digital versatile disk (DVD) target is presented as a matrix-free sample support for application in laser desorption/ionization mass spectrometry (LDI-MS). large number of vacancies, defects, relative sp2 content, and nanogrooves DLC films the LDI phenomenon. The observed absorptivity range 305−330 nm (nitrogen laser, 337 nm). universal applicability demonstrated through different analytes like amino acids, carbohydrates, lipids, peptides,...
In this study, a new type of immobilized metal-ion affinity chromatography (IMAC) resin for the isolation phosphopeptides was synthesized which is based on specific interaction between phosphate groups and chelated lanthanide metal ions. regard trivalent lanthanum, holmium erbium ions were to highly porous phosphonate polymer prepared by radical polymerization vinylphosphonic acid (VPA) divinylbenzene (DVB). The developed method evaluated with peptide mixtures from digested standard proteins...
Truffles represent the best known and most expensive edible mushroom. Known as Ascomycetes, they belong to genus Tuber live in symbiosis with plant host roots. Due their extraordinary taste smell, truffles are sold worldwide for high prices of up 3000-5000 euros per kilogram (Tuber magnatum PICO). Amongst black truffles, species melanosporum VITTAD. is highly regarded its organoleptic properties. Nonetheless, numerous different sorts truffle offered at lower prices, including...
Over the past couple of years, proteomics pattern analysis has emerged as an effective method for early diagnosis diseases such ovarian, breast, or prostate cancer, without identification single biomarkers. MALDI-TOF MS, example, offers a simple approach fast and reliable protein profiling, especially by using carrier materials with various physical chemical properties, in combination MALDI matrix. This is referred to material-enhanced laser desorption/ionization (MELDI). In this paper, we...
A metal–organic framework, consisting of Er(III) linked together by 1,4-phenylenediacetate, was synthesised a one-pot reaction and successfully used as an affinity material for the selective capturing phosphopeptides. An optimised protocol loading, washing elution developed eluents were analysed via matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. standard protein digest (α-casein, β-casein ovalbumin) well digested egg white proteins to test efficiency...
E-liquids have become increasingly popular in society recent years. A wide variety of flavors and nicotine strengths make it possible for every user to get a product according their wishes. Many these e-liquids are marketed with countless different flavors, which often characterized by strong sweet smell. Sweeteners, such as sucralose, therefore commonly added sugar substitutes. However, studies shown the potential formation highly toxic chlorinated compounds. This can be explained high...
60]fullerene derivatives (dioctadecyl methano[60]fullerene, [60]fullerenoacetic acid, and IDA-[60]fullerene) were prepared subjected to a comprehensive characterization study including protein binding properties capacity. These fullerene successfully applied as material-enhanced laser desorption/ionization (MELDI) carrier materials. It is shown that diverse functionalities result in characteristic human serum peak patterns (m/z 2000-20 000) terms of signal intensity well the number...
This review covers recent applications of near infrared (NIR) spectroscopy in the determination physico-chemical and morphological parameters polymeric materials. Near measurements diffuse reflection mode are highlighted, which analyse structural such as porosity, surface area particle size. Fundamentals technique discussed examples quantitative qualitative analysis explained. Various approaches like on- in-line techniques, bulk kinetic studies for recording spectra discussed. Furthermore,...
Trypsin was immobilized on glycidylmethacrylate-co-divinylbenzene (GMA/DVB) polymerized in pipet tips for online enzymatic digestion of proteins. The major advantages in-tip are easy handling and small sample amount required analysis. Microwave-assisted applied highly efficient time saving proteolysis. Adaption to an automated robotic system allowed fast reproducible treatment. Investigations with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS)...
Abstract In spite of the growing acceptance matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for analysis a wide variety compounds, including polymers and proteins, its use in analyzing low‐molecular‐weight molecules (<1000 m / z ) is still limited. This mainly due to interference matrix low‐mass range. Here derivatized fullerenes covalently bound silica particles with different pore sizes are applied as thin layer (LDI) spectrometric analysis. Thus, an...
Anaerobic gut fungi (AGF) have emerged as promising candidates for optimized biogas and biofuel production due to their unique repertoire of potent lignocellulose-degrading enzymes. However, identifying AGF strains through standard fungal DNA barcodes still poses challenges distinct genomic features. This study explored the applicability matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI) direct analysis in real-time (DART) (MS) alternative methods...