A5080975932- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Pesticide Exposure and Toxicity
- Antibiotic Resistance in Bacteria
- Tissue Engineering and Regenerative Medicine
- Oral Health Pathology and Treatment
- Skin Protection and Aging
- Bee Products Chemical Analysis
- Lipoproteins and Cardiovascular Health
- Peptidase Inhibition and Analysis
- Insect and Pesticide Research
- Machine Learning in Bioinformatics
- Genomics, phytochemicals, and oxidative stress
- Immunotoxicology and immune responses
- Oral microbiology and periodontitis research
- Photodynamic Therapy Research Studies
- Antimicrobial Peptides and Activities
- Nicotinic Acetylcholine Receptors Study
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Genetics and Neurodevelopmental Disorders
- Nephrotoxicity and Medicinal Plants
- Protein purification and stability
- Cholesterol and Lipid Metabolism
- Animal testing and alternatives
International Center for Chemical and Biological Sciences
2013-2025
University of Karachi
2012-2025
Bahauddin Zakariya University
2021
Universität Innsbruck
2012-2019
Abstract Extracellular matrix‐based bio‐scaffolds are useful for tissue engineering as they retain the unique structural, mechanical, and physiological microenvironment of thus facilitating cellular attachment matrix activities. However, considering its potential, a comprehensive understanding protein profile remains elusive. Herein, we evaluate impact decellularization on human amniotic membrane (hAM) based proteome profile, physicochemical features, well attachment, viability,...
In this study, a new type of immobilized metal-ion affinity chromatography (IMAC) resin for the isolation phosphopeptides was synthesized which is based on specific interaction between phosphate groups and chelated lanthanide metal ions. regard trivalent lanthanum, holmium erbium ions were to highly porous phosphonate polymer prepared by radical polymerization vinylphosphonic acid (VPA) divinylbenzene (DVB). The developed method evaluated with peptide mixtures from digested standard proteins...
A metal–organic framework, consisting of Er(III) linked together by 1,4-phenylenediacetate, was synthesised a one-pot reaction and successfully used as an affinity material for the selective capturing phosphopeptides. An optimised protocol loading, washing elution developed eluents were analysed via matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. standard protein digest (α-casein, β-casein ovalbumin) well digested egg white proteins to test efficiency...
Hepatocellular carcinoma is one of the leading causes death with a higher prevalence in males compared to females due distinct progression and pathogenesis patterns both genders. This study aimed at creating, characterizing, proteome profiling, DEN-induced HCC model male female Balb/c mice. Proteins were extracted from characterized mouse models subjected mass spectrometry subsequent bioinformatics analysis. The results revealed that differentially enriched signaling pathways treated mice...
The basic idea of this study was to recover phosphopeptides after trypsin-assisted digestion precipitated phosphoproteins using trivalent lanthanide ions. In the first step, were extracted from protein solution by precipitation with La3+ and Ce3+ ions, forming stable pellets. Additionally, lanthanide–phosphoprotein complexes suspended directly digested on-pellet trypsin. Non-phosphorylated peptides released into supernatants enzymatic cleavage remained bound on pellet. Further washing steps...
Beta cell apoptosis induced by proinflammatory cytokines is one of the hallmarks diabetes. Small molecules which can inhibit cytokine-induced could lead to new drug candidates that be used in combination with existing therapeutic interventions against The current study evaluated several effects bergenin, an isocoumarin derivative, beta cells presence cytokines. These included (i) increase viability (by measuring cellular ATP levels) (ii) suppression caspase activity), (iii) improvement...
Scorpion venoms are known to contain over 100,000 biologically active constituents. However, only a few of them have been studied. The major constituents venom proteins and peptides, which exhibit various biological pharmacological properties, including anticancer activities. In the current study, yellow scorpions ( Buthus sindicus ) found in Sindh, Pakistan, was extracted evaluated for its anti-cancer anti-inflammatory crude showed dose dependent inhibition phagocyte oxidative burst from...
Organic acid disorders are rare inherited metabolic of key pathways. For the identification specific organic acids, investigation urinary metabolites and genetic testing required through newborn screening programmes. Delayed diagnosis leads to complications, such as cardiac attacks, respiratory problems, neuro-developmental disorders, intellectual disability, even premature death. The burden is quite high in developing countries South Asia due rate consanguinity region. Unfortunately, left...
Abstract This study was designed to investigate the prevalence and associated risk factors of Shigella flexneri isolated from drinking water retail raw food samples in Peshawar, Pakistan. A total 1,020 different were collected various areas Peshawar between January 2016 May 2017, followed by identification S. through biochemical, serological, 16S rRNA gene sequencing. Potential with development spreading infection also investigated. Overall, 45 (4.41%) positive for species. Among these...
Abstract In the current study, APOB ( rs1052031) genotype-guided proteomic analysis was performed in a cohort of Pakistani population. A total 700 study subjects, including Coronary Artery Disease (CAD) patients (n = 480) and healthy individuals 220) as control group were included study. Genotyping carried out by using tetra primer-amplification refractory mutation system-based polymerase chain reaction (T-ARMS-PCR) whereas mass spectrometry (Orbitrap MS) used for label free quantification...
ABSTRACT Acinetobacter baumannii is a Gram-negative pathogen, accountable for lethal nosocomial infections, owing to high pathogenicity and multi-drug resistance (MDR). Hence, the World Health Organization has prioritized this bacterium against which new pharmacophores are required urgently. Therefore, study evaluates antimicrobial potential of lithium complex based on salicylic acid, 1,10-phenanthroline {[Li(phen) 2 sal]} MDR A. (MDRAB) through growth inhibition, curve assay, biofilm...
Abstract Ultraviolet-A (UVA) radiation is a major contributor to reactive oxygen species (ROS), nitrite (RNS), inflammation, and DNA damage, which causes photoaging photocarcinogenesis. This study aimed evaluate the UVA protective potential of lipophilic chain conjugated thiourea-substituted aryl group molecules against UVA-induced cellular damages in human dermal fibroblasts (BJ cell line). We tested series nineteen (19) for photoprotection, from 2′,5′-dichlorophenyl-substituted molecule...
Objective: To determine the molecular strain typing and drug resistance pattern of Salmonella enterica serovar Typhi prevalent in Northwest Pakistan. Methodology: A total 2,138 blood samples suspected typhoid patients from Pakistan were collected followed by identification through biochemical, serological, species-specific fliC-d gene amplification. These isolates typed variable-number tandem repeat (VNTR) profiling investigated for resistance. Results: The overall prevalence was found to be...
The MS data revealed a differential expression of proteins due to prolonged betel nut consumption, and their potential role in inflammation or protection.
Abstract A poly(dibenzo‐18‐crown‐6) was used as a new solid‐phase extraction material for the selective enrichment of phosphopeptides. Isolation phosphopeptides achieved based on specific ionic interactions between and phosphate group Thus, method developed optimized, including loading, washing elution steps, To assess this potential, tryptic digest three proteins ( α ‐ casein, β ‐casein ovalbumin) applied poly(dibenzo‐18‐crown‐6). The nonspecific products were removed by centrifugation...
Abstract Background; Dinotefuran is a new class of neonicotinoids claimed to be harmless mammals and humans. This claim was daunted by the documented effect dinotefuran on honeybees further studies were required. Aim: The study designed assess capaciousness damage caused prolonged exposure in probable strategy neutralize its effect. Methodology: Ninety-day trial using Wistar rats (n=45) conducted while dividing them into three groups: untreated control group, insecticide (dinotefuran)...