Echinococcus granulosus is characterized by high intra-specific variability (genotypes G1–G10) and according to the new molecular phylogeny of genus Echinococcus, E. complex has been divided into sensu stricto (G1–G3), equinus (G4), ortleppi (G5), canadensis (G6–G10). The characterization isolates fundamental understand spatio-temporal epidemiology this in many endemic areas with simultaneous occurrence different species genotypes. To simplify genotyping we developed a single-tube multiplex...

The protein P29 is a potential serological marker for post-treatment monitoring of cystic echinococcosis (CE) especially in young patients. We now have demonstrated that encoded the Echinococcus genus by single gene consisting 7 exons spanning 1.2 kb DNA. Variability p29 at inter- and intra-species level was assessed with 50 cDNA 280 genomic DNA clones isolated from different E. granulosus s.l. isolates (E. sensu stricto (G1), equinus (G4), ortleppi (G5), canadensis (G6), (G7) (G10)) as well...

Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recCL1), were assessed individually in combination enzyme-linked immunosorbent assays (ELISA) for the specific serodiagnosis of human fasciolosis areas low endemicity as encountered Central Europe. Antibody detection was conducted using ProteinA/ProteinG (PAG) conjugated to alkaline phosphatase. Test characteristics well agreement with results from an ELISA excretory–secretory products (FhES)...

A structure activity relationship (SAR) study of a library 56 compounds (54 ruthenium and 2 osmium derivatives) based on the trithiolato-bridged dinuclear ruthenium(II)-arene scaffold (general formula [(η6-arene)2Ru2(μ2-SR)3]+, symmetric [(η6-arene)2Ru2(μ2-SR1)2(μ2-SR2)]+, mixed, respectively) is reported. The (of which 34 are newly designed drug candidates) were synthesized by introducing chemical modifications at level bridge thiols, they grouped into eight families according to their...

To compare the performance of two novel recombinant antigens (EgP29, 2B2t) with imaging in a well-defined cohort surgically treated cystic echinococcosis (CE) patients to determine whether serology reflects surgical cure as defined by imaging.From 223 CE-confirmed national clinical center for echinococcosis, 36 were eligible analysis. Sera tested enzyme-linked immunosorbent assay (ELISA) specific IgG and IgG4 antibodies against EgP29 2B2t antigens. We used hierarchical linear regression...

The synthesis, characterization, photophysical and biological properties of 13 new conjugate coumarin-diruthenium(II)⋅arene complexes against Toxoplasma gondii are presented. For all organometallic unit/coumarins, an almost complete loss fluorescence efficacy was observed. However, the nature fluorophore, type bonding, presence length a linker between coumarin dye ruthenium(II) moiety, number units influenced their properties. in vitro activity transgenic T. strain grown human foreskin...

The synthesis, characterization, and in vitro antiparasitic anticancer activity evaluation of new conjugates containing two three dinuclear trithiolato-bridged ruthenium(II)-arene units are presented. Antiparasitic was evaluated using transgenic Toxoplasmagondii tachyzoites constitutively expressing β-galactosidase grown human foreskin fibroblasts (HFF). compounds inhibited T.gondii proliferation with IC50 values ranging from 90 to 539 nM, seven derivatives displayed lower than the reference...

Toxoplasma gondii is an apicomplexan parasite that infects and proliferates within many different types of host cells virtually all warm-blooded animals humans. Trypanosoma brucei extracellular kinetoplastid causes human African trypanosomiasis Nagana disease in cattle, primarily rural sub-Saharan Africa. Current treatments against both parasites have limitations, e.g., suboptimal efficacy adverse side effects. Here, we investigate the potential cellular molecular targets a...

Leucinostatins are antimicrobial peptides with a broad range of activities against infectious agents as well mammalian cells. The leucinostatin-derivative peptide ZHAWOC_6027 (peptide 6027) was tested in vitro and vivo for activity the intracellular apicomplexan parasite Toxoplasma gondii. While highly efficacious (EC50 = 2 nM), subcutaneous application 6027 (3 mg/kg/day 5 days) mice experimentally infected T. gondii oocysts exacerbated infection, caused mild clinical signs elevated cerebral...

Alveolar echinococcosis (AE) is caused by infection with the larval stage of tapeworm Echinococcus multilocularis. An increasing understanding immunological events that account for metacestode survival in human and murine AE prompted us to undertake explorative experiments tackling potential novel preventive and/or immunotherapeutic measures. In this study, immunoprotective ability recombinant EmP29 antigen (rEmP29) was assessed mice were intraperitoneally infected E. multilocularis...

In T. gondii, as well in other model organisms, gene knock-out using CRISPR-Cas9 is a suitable tool to identify the role of specific genes. The general consensus implies that only interest affected by knock-out. Is this really case? previous study, we generated (KO) clones TgRH88_077450 (SRS29B; SAG1) which differed numbers integrated dihydrofolate-reductase-thymidylate-synthase (MDHFR-TS) drug-selectable marker. Clones 18 and 33 had single insertion MDHFR-TS within SRS29B. Clone 6 was...

Parts of the natural life cycle Echinococcus granulosus can be retraced in vitro such as development protoscoleces into semiadult worms with three or more proglottids, redifferentiation cultured metacestode-like cystic structures. Most generated samples share-at microscopical level-high similarities those naturally grown, but developmental differences have also been documented, missing egg production grown adults unusual bladder/vesicle formation metacestode direction. The aim present study...

Eight novel carbohydrate-tethered trithiolato dinuclear ruthenium(II)-arene complexes were synthesized using CuAAC ‘click’ (Cu(I)-catalyzed azide-alkyne cycloaddition) reactions, and there in vitro activity against transgenic T. gondii tachyzoites constitutively expressing β-galactosidase (T. β-gal) non-infected human foreskin fibroblasts, HFF, was determined at 0.1 1 µM. When evaluated µM, seven diruthenium-carbohydrate conjugates strongly impaired parasite proliferation by >90%, while HFF...

Trithiolato-bridged dinuclear ruthenium(II)-arene complexes are active against various parasites including Toxoplasma gondii. Lipids, isoprenoids and lipoate metabolites scavenged by T. gondii from the host cell but also synthesized parasite, these molecules can be appended to diruthenium moiety in a conjugate approach aiming at compounds with improved antiparasitic activity selectivity. The synthesis vitro evaluation of 23 new trithiolato bearing lipophilic units reported. influence several...

We present a comprehensive analysis of the hepatic miRNA transcriptome at one month post-infection experimental primary alveolar echinococcosis (AE), parasitic infection caused upon ingestion E. multilocularis eggs. Liver tissues were collected from infected and non-infected C57BL/6 mice, then small RNA libraries prepared for next-generation sequencing (NGS). conducted Stem-loop RT-qPCR validation most dysregulated miRNAs. In expression levels 28 miRNAs significantly altered. Of these, 9...

Abstract The synthesis, photophysical properties and antiparasitic efficacy against Toxoplasma gondii β‐gal (RH strain tachyzoites expressing β‐galactosidase) grown in human foreskin fibroblast monolayers (HFF) of a series 15 new conjugates BODIPY‐trithiolato‐bridged dinuclear ruthenium(II)‐arene complexes are reported (BODIPY=4,4‐difluoro‐4‐bora‐3 ,4 ‐diaza‐ s ‐indacene, derivatives used as fluorescent markers). influence the bond type (amide vs . ester), well that length nature (alkyl...

Tethering known drugs to a metalorganic moiety is an efficient approach for modulating the anticancer, antibacterial, and antiparasitic activity of organometallic complexes. This study focused on synthesis evaluation new dinuclear ruthenium(II)–arene compounds linked several antimicrobial such as dapsone, sulfamethoxazole, sulfadiazine, sulfadoxine, triclosan, metronidazole, ciprofloxacin, well menadione (a 1,4-naphtoquinone derivative). In primary screen, 30 (17 hybrid molecules,...

In T. gondii, as well in other model organisms, gene knock-out using CRISPR-Cas9 is a suitable tool to identify the role of specific genes. The general consensus implies that only interest affected by knock-out. Is this really case? previous study, we have generated (KO) clones TgRH88_077450 (SRS29B; SAG1) which differed numbers integrated dihydrofolate-reductase-thymidylate-synthase (MDHFR-TS) drug-selectable marker. Clones 18 and 33 had single insertion MDHFR-TS within SRS29B. Clone 6 was...