A5033975424- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Telomeres, Telomerase, and Senescence
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Reproductive System and Pregnancy
- Asthma and respiratory diseases
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Physiology in Livestock
- Lipoproteins and Cardiovascular Health
- Acute Myocardial Infarction Research
- Lipid Membrane Structure and Behavior
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
- Chalcogenide Semiconductor Thin Films
- Atherosclerosis and Cardiovascular Diseases
- Neurotransmitter Receptor Influence on Behavior
- Neuropeptides and Animal Physiology
- Mitochondrial Function and Pathology
- Cardiovascular Issues in Pregnancy
- Pluripotent Stem Cells Research
- Receptor Mechanisms and Signaling
Chinese Academy of Sciences
2015-2025
University of Pennsylvania
1992-2025
Shanghai Institute of Materia Medica
2025
Institute of Zoology
2014-2024
Changzhi Medical College
2020-2024
Second Hospital of Anhui Medical University
2024
Anhui Medical University
2024
Karolinska Institutet
2024
Tianjin People's Hospital
2024
University of Chinese Academy of Sciences
2017-2021
The chitinase-like protein YKL-40 is involved in inflammation and tissue remodeling. We recently showed that serum levels were elevated patients with asthma correlated severity, thickening of the subepithelial basement membrane, pulmonary function. hypothesized single-nucleotide polymorphisms (SNPs) affect also influence status lung function.We carried out a genomewide association study founder population European descent, Hutterites, then tested for an between implicated SNP One associated...
The G-rich strand of human telomeric DNA can fold into a four-stranded structure called G-quadruplex and inhibit telomerase activity that is expressed in 85−90% tumor cells. For this reason, telomere quadruplex emerging as potential therapeutic target for cancer. Information on the physiological environment important structure-based drug design targeting quadruplex. Recent studies have raised significant controversy regarding exact formed by relevant environment. Studies crystal prepared K+...
Large numbers of guanine-rich sequences with potential to form G-quadruplexes have been identified in genomes various organisms. Such are constrained at both ends by long DNA duplex a complementary strand close proximity compete for formation. G-quadruplex/duplex competition double-stranded has rarely studied. In this work, we used DMS footprinting and gel electrophoresis study G-quadruplex formation derived from human genome under dilute molecular crowding condition created PEG. was...
Abstract G-quadruplex (G4) structures formed by guanine-rich nucleic acids are implicated in essential physiological and pathological processes serve as important drug targets. The genome-wide detection of G4s living cells is for exploring the functional role but has not yet been achieved due to lack a suitable G4 probe. Here we report an artificial 6.7 kDa probe (G4P) protein that binds with high affinity specificity. We used it capture human, mouse, chicken ChIP-Seq technique, yielding...
Significance Guanine-rich nucleic acids fold into a four-stranded structure named G-quadruplex that has implications in essential cellular processes, pharmaceutical applications, and nanodevices. We found unique type of contains G-vacancy is stabilized by guanine derivatives such as te physiological concentration GTP fill-in at the base. In response to changes derivatives, this able manipulate tracking activity protein on DNA exemplified polymerase-catalyzed synthesis. Because are natural...
G-quadruplex formation in genomic DNA is considered to regulate transcription. Previous investigations almost exclusively focused on intramolecular G-quadruplexes formed by carrying four or more G-tracts, and structure has rarely been studied physiologically relevant processes. Here, we report an entirely neglected, but actually much prevalent form of G-quadruplexes, DNA:RNA hybrid (HQ) that forms HQ requires as few two G-tracts instead a non-template strand. Potential sequences (PHQS) are...
Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form sporadic Parkinson's disease (PD), respectively, which manifested the selective vulnerability neuronal cells in substantia nigra (SN) and striatum (STR) regions. However, underlying molecular mechanism linking parkin etiology PD remains elusive. Here we report that p62, a critical regulator for protein quality control, inclusion body formation, autophagy diverse signaling pathways, is new substrate...
HLA-G is a class Ib HLA gene with unique tissue expression pattern and immunomodulatory properties. Polymorphisms in the promoter region have been associated miscarriage asthma, whereas levels wide range of pathologic conditions as well survival embryos after vitro fertilization organs transplantation. Here, we characterize sequence variation haplotype structure flanking sequences 44 African Americans, 47 European Americans 43 Han Chinese by haplotype-specific PCR sequencing. In all three...
Plasma lipoprotein(a) (Lp[a]) level is an independent risk factor of cardiovascular disease that under strong genetic control. We conducted a genome-wide association study plasma Lp(a) in 386 members founder population adheres to communal lifestyle, proscribes cigarette smoking, and prepares eats meals communally. identified associations with 77 single nucleotide polymorphisms (SNPs) spanning 12.5 Mb on chromosome 6q26-q27 met criteria for significance (P <or= 1.3 x 10(-7)) were within or...
We recently reported that a DNA:RNA hybrid G-quadruplex (HQ) forms during transcription of DNA bears two or more tandem guanine tracts (G-tract) on the nontemplate strand. Putative HQ-forming sequences are enriched in nearby 1000 nt region right downstream start sites strand warm-blooded animals, and HQ regulates under both vitro vivo conditions. Therefore, knowledge mechanism formation is important for understanding biological function as well manipulating gene expression by targeting HQ....
Significance Our work reveals the genome-wide prevalence of G4 structural variations (G4Vs) associated with single-nucleotide (SNVs) in human genome and how such G4Vs interact gene-regulatory elements subsequently affect activities genes accommodating G4Vs. The are enriched around transcription start sites, implying their potential involvement gene expression. Because structures participate essential physiological pathological processes, should make crucial contributions to genotype...
Impact of crowding: Molecular crowding with poly(ethylene glycol) (PEG) induces the formation a G-quadruplex structure in guanine-rich strand telomeric DNA at K+-ion concentration that is unable to induce alone. The picture shows folding and unfolding DNA, revealed by fluorescence resonance energy transfer (FRET), efficiency which inversely dependent on distance between donor (D) acceptor (A).
Nucleic acid molecules may fold into secondary structures, and the formation of such structures is involved in many biological processes technical applications. The folding unfolding rate constants define kinetics conformation interconversion stability these important realizing their functions. We developed a method to determine kinetic parameters using an optical biosensor based on surface plasmon resonance. nucleic coupled with hybridization reaction by immobilization target sensor chip...
Abstract Water‐soluble, octacationic zinc phthalocyanine (ZnPc) was found to be a very good G‐quadruplex DNA stabilizer by using UV‐melting studies and polymerase stop assays, potent telomerase inhibitor the telomeric repeat amplification protocol (TRAP) assay. The compound’s DNA‐binding properties were also studied surface plasmon resonance (SPR). Furthermore, CD experiments demonstrated that ZnPc could induce intramolecular structure transition from antiparallel parallel form. More...
Human chromosome ends are protected with kilobases repeats of TTAGGG. Telomere DNA shortens at replication. This shortening in most tumor cells is compensated by telomerase that adds telomere to the 3' end G-rich strand. Four TTAGGG can fold into G-quadruplex a poor substrate for telomerase. property has been suggested regulate activity vivo and inhibition via stabilization considered therapeutic strategy against cancer. Theoretically form anywhere along long Where forms determines whether...
BACKGROUND: HLA-G is a non-classical HLA with important immunomodulatory roles in pregnancy. A polymorphism the promoter region, –725G, was previously associated sporadic miscarriage women who were unselected respect to reproductive history. In this study, transcription levels of different haplotypes examined determine whether miscarriage-associated –725G allele influences transcription. METHODS: Five naturally occurring and three variant created by site-directed mutagenesis sub-cloned into...
Telomere DNA in human cells shortens during each round of replication. In cancer cells, telomere shortening is compensated by telomerase or the alternative lengthening (ALT) mechanism to maintain cell division potential. The G-rich strand can fold into a G-quadruplex structure and disrupt these two processes. Therefore, stabilization chemical ligands emerging as promising anticancer strategy. So far, vitro studies on such are exclusively carried out dilute solutions. However, intracellular...
Recently, we reported the co-transcriptional formation of DNA:RNA hybrid G-quadruplex (HQ) structure by non-template DNA strand and nascent RNA transcript, which in turn modulates transcription under both vitro vivo conditions. Here present bioinformatic analysis on putative HQ-forming sequences (PHQS) genomes eukaryotic organisms. Starting from amphibian, PHQS motifs are concentrated immediate 1000-nt region downstream start sites, implying their potential role regulation. Moreover,...
G-quadruplexes, four-stranded structures formed by Guanine-rich nucleic acids, are implicated in many physiological and pathological processes. G-quadruplex-forming sequences abundant genomic DNA, G-quadruplexes have recently been shown to exist the genome of mammalian cells. However, how genomes remains largely unclear. Here, we show that G-quadruplex formation can be remotely induced downstream transcription events thousands base pairs away. The alter protein recognition cause termination...
Human mitochondrial DNA contains a distinctive guanine-rich motif denoted conserved sequence block II (CSB II) that stops RNA transcription, producing prematurely terminated transcripts to prime replication. Recently, we reported general phenomenon DNA:RNA hybrid G-quadruplexes (HQs) readily form during transcription when the non-template strand is and such HQs in turn regulate transcription. In this work, show of leads formation stable HQ or alternatively an unstable intramolecular...