A5063761368- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Animal Disease Management and Epidemiology
- Listeria monocytogenes in Food Safety
- Plant Pathogens and Fungal Diseases
- Microbial infections and disease research
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Peptidase Inhibition and Analysis
- Bacillus and Francisella bacterial research
- Viral Infections and Vectors
- Identification and Quantification in Food
- Biochemical and Structural Characterization
- Cytomegalovirus and herpesvirus research
- Vector-borne infectious diseases
- Microbial Metabolism and Applications
- Antimicrobial Resistance in Staphylococcus
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- T-cell and Retrovirus Studies
- Bacterial biofilms and quorum sensing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Infectious Diseases and Mycology
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2019-2024
Centre Hospitalier de Niort
2024
CEA Paris-Saclay
2021
Bio-Rad (France)
2002-2015
Bio-Rad (United States)
2004-2008
Inserm
2002-2008
Centre National de la Recherche Scientifique
2008
Sorbonne Université
2002
Hôpital Saint-Antoine
2002
Sanofi (France)
1996-1999
Invasive aspergillosis (IA) is a frequent complication of blood or marrow transplantation. Previous studies have reported that the Aspergillus galactomannan enzyme immunoassay (GM EIA) may be useful diagnostic tool for IA, but its sensitivity variable. We examined performance GM EIA in 986 serum samples from 67 patients. Results demonstrated decreasing index cutoff positivity to 0.5 increased sensitivity, with minimal loss specificity. The low duration test before diagnosis by clinical...
ABSTRACT Two standardized enzyme immunoassays for the serological diagnosis of candidiasis were developed. The first one detects antimannan antibodies, while second mannan with a sensitivity 0.1 ng/ml. These tests applied to 162 serum samples retrospectively selected from 43 patients mycologically and clinically proven caused by Candida albicans . Forty-three positive mannan, 63 had significant antibody levels. Strikingly, only five simultaneously both tests. When results analyzed per...
Many health care centers worldwide use the Platelia Aspergillus enzyme immunoassay (PA-EIA; Bio-Rad Laboratories) for diagnosis of invasive aspergillosis (IA). A cutoff optical density (OD) index 1.5 was originally recommended by manufacturer, but in practice, most institutions lower values. Moreover, a OD 0.5 recently approved United States. In present study, we set out to optimize level performing retrospective analysis PA-EIA values samples that had been obtained prospectively from adult...
A novel strategy for the diagnosis of systemic candidosis was evaluated, based on combination two enzyme immunoassays that detect a candida oligomannoside repetitive epitope expressed in large amounts by Candida albicans (Platelia Ag), and antibodies against C. mannan, major cell-wall immunogen which this is present Ab). Sera were selected retrospectively from intensive care haematology patients with clinically suspected candidosis, whom spp. had been isolated normally sterile sites. Of 21...
ABSTRACT Galactofuranose-containing molecules have been repeatedly shown to be important antigens among human fungal pathogens, including Aspergillus fumigatus . Immunogenic galactofuran determinants poorly characterized chemically, however. We reported here the characterization of two glycoproteins A. with an N-glycan containing galactofuranose. These proteins are a phospholipase C and phytase. Chemical indicates that it is mixture Hex 5-13 HexNAc 2 oligosaccharides, major molecular species...
Journal Article Tissue culture assays using Caco‐2 cell line differentiate virulent from non‐virulent Listeria monocytogenes strains Get access Van Langendonck, Langendonck Institut National de la Recherche Agronomique Tours, Laboratoire Pathologie Infectieuse et Immunologie, Nouzilly, France Search for other works by this author on: Oxford Academic Google Scholar Bottreau, Bottreau Bailly, Bailly Tabouret, Tabouret Marly, Marly Pardon, Pardon Velge of Applied Microbiology, Volume 85, Issue...
ABSTRACT An enzyme immunoassay (EIA)—the commercially available Platelia Candida antigen test—developed for the diagnosis of systemic candidiasis is based on detection α-linked oligomannose residues (α-Man) released from cells into serum. This test has good specificity but to be repeated frequently because rapid clearance detectable mannanemia. We have developed a second EIA beta-linked oligomannoses (β-Man), since β-Man are linked different molecules and interact differently with host...
Summary The virulence of 74 Listeria monocytogenes isolates from clinical cases and food products 11 other species was tested in mice immunocompromised with carrageenan. Isolates than L. were not lethal to such mice. All 29 (serotypes 1/2a, 1/2b, 4b) 33 42 various serotypes isolated mainly dairy killed all test (100% lethality) at an inoculum 104 cfu/mouse. strains haemolytic possessed the 58-Kda band specific for listeriolysin O as demonstrated by SDS-PAGE immunoblotting. nine avirulent had...
Summary: SDS extracts of whole bacteria, representing five species and 15 serovars Listeria, were analysed by SDS-PAGE immunoblotting with serum directed against formalin-treated L. monocytogenes. Profiles monocytogenes very different from those other Listeria (i.e. innocua, welshimeri, seeligeri ivanovii). This low degree similarity between was found even in the case common serovars. Within monocytogenes, protein patterns characterized, on one hand, a high homogeneity all strains same...
As a part of our glycoantigen synthetic program for diagnosis and basic analysis yeast-related pathogenic mechanisms, library 1-->2 oligomannosides suitable immunoanalysis was prepared. The use biotin sulfone, an oxidized form biotin, offers convenient solution both oligosaccharide synthesis immobilization on microspheres surface plasmon resonance sensors. application this new strategy the anti- Candida albicans antibody response through multiple-analyte profiling technology (Luminex) with...
Background The Platelia Aspergillus Ag assay (Bio-Rad) is designed for detecting galactomannan (GM) and widely used diagnosing invasive aspergillosis but hampered by variable occurrences of unreproducible positive results. Frequency origin these results have not been formally studied. Methods Different technicians simultaneously performed four tests on 550 consecutive sera from adult patients (Test#1-Test#2 extraction#1 Test#3-Test#4 extraction#2). samples were classified as confirmed...
Pooled serum testing using whole-virus indirect ELISA has been recently recognized as an official method for surveillance of bovine herpesvirus 1 (BoHV1) in cattle herds Europe. In this study, a retrospective analysis data from the French BoHV1 campaign 2018-2019, including 7434 BoHV1-free certified and 157 infected herds, was performed order to evaluate diagnostic specificity sensitivity two pooled ELISAs (from IDEXX IDVet), comparison with individual by blocking targeting gB gE proteins....
Summary An enzyme-linked immunosorbent assay (ELISA) consisting of a double sandwich technique with rabbit and sheep antibodies, was developed for the detection cytotoxic necrotising factor (CNF) in extracts Escherichia coli strains. The evaluated by comparison results obtained an based on toxicity HeLa cell cultures. In study 27 CNF+ 45 CNF- strains ultrasonic disintegration, no false positive only three negative were recorded; latter that produced less CNF than any others examined....
Pooled-serum testing using whole-virus indirect ELISA has been recently recognized as an official method for surveillance of bovine herpesvirus 1 (BoHV1) in cattle herds Europe, provided its sensitivity allows the detection one weak positive sample pool. Testing pools size up to 10 sera is currently use French BoHV1 eradication program, combination with confirmatory individual analyses by gB and gE blocking ELISAs. In this retrospective study, data from campaign 2018-2019 (157 infected 7,434...