A5109330024- Antifungal resistance and susceptibility
- Parasitic Infections and Diagnostics
- Fungal Infections and Studies
- Amoebic Infections and Treatments
- Plant Pathogens and Fungal Diseases
- Toxoplasma gondii Research Studies
- Indoor Air Quality and Microbial Exposure
- Coccidia and coccidiosis research
- Probiotics and Fermented Foods
- Adrenal and Paraganglionic Tumors
- Pneumocystis jirovecii pneumonia detection and treatment
- Essential Oils and Antimicrobial Activity
- Neutropenia and Cancer Infections
- Viral gastroenteritis research and epidemiology
- Adrenal Hormones and Disorders
- Legionella and Acanthamoeba research
- Infectious Diseases and Mycology
- Gut microbiota and health
- Vector-borne infectious diseases
- Parasitic Diseases Research and Treatment
- Mycobacterium research and diagnosis
- Allergic Rhinitis and Sensitization
- Bacterial Identification and Susceptibility Testing
- Radiation Dose and Imaging
- HIV/AIDS oral health manifestations
Université de Rouen Normandie
2025
Maison des Sciences de l’Homme de Dijon
1987-2021
CHU Dijon Bourgogne
2009-2018
Institut Agro Dijon
2018
Procédés Alimentaires et Microbiologiques
2016-2017
Université de Bourgogne
1987-2016
Université Bourgogne Franche-Comté
2016
Agroécologie
2011-2015
Inserm
2002-2015
Université Paris-Sud
2015
PURPOSE The prognosis of invasive pulmonary aspergillosis (IPA) occurring in neutropenic patients remains poor. We studied whether new strategies for early diagnosis could improve outcome these patients. PATIENTS AND METHODS Twenty-three histologically proven and 14 highly probable IPAs 37 hematologic (neutropenic 36) were analyzed retrospectively. RESULTS most frequent clinical signs associated with IPA cough (92%), chest pain (76%), hemoptysis (54%). Bronchoalveolar lavage (BAL) was...
The human pathogenic fungus Candida albicans can cause systemic infections by invading epithelial barriers to gain access the bloodstream. One of main reservoirs C. is gastrointestinal tract and predominantly originate from this niche. In study, we used scanning electron fluorescence microscopy, adhesion, invasion damage assays, fungal mutants a set host cell inhibitors investigate interactions with oral cells enterocytes. Our data demonstrate that depend not only on morphology activity, but...
A novel strategy for the diagnosis of systemic candidosis was evaluated, based on combination two enzyme immunoassays that detect a candida oligomannoside repetitive epitope expressed in large amounts by Candida albicans (Platelia Ag), and antibodies against C. mannan, major cell-wall immunogen which this is present Ab). Sera were selected retrospectively from intensive care haematology patients with clinically suspected candidosis, whom spp. had been isolated normally sterile sites. Of 21...
Conventional identification (CI) of yeasts is based on morphological, biochemical and/or immunological methods. Matrix-assisted laser desorption/ionization – time flight (MALDI-TOF or MT-MS) mass spectrometry has been proposed as a new method for the microorganisms. This prospective study compared performance MT-MS and CI isolated from clinical samples. Sequencing internal transcribed spacer (ITS) regions ribosomal DNA was used reference in analysis total 1207 yeast isolates. Concordance...
In order to define transmission routes of cryptosporidiosis and develop markers that distinguish Cryptosporidium parvum isolates, we have identified 2 polymorphic restriction enzyme sites in a C. repetitive DNA sequence. The target sequence was amplified by polymerase chain reaction from 100 500 oocysts the product subjected digestion. Typing 23 isolates showed 10 / calf had same profile. contrast, patterns were observed among human isolates: 7 13 displayed profile, 6 presented another...
ABSTRACT We report a case of cryptococcosis in which serum enzyme-linked immunosorbent assay (ELISA) for Aspergillus galactomannan was positive, with no evidence aspergillosis. Soluble antigens from 19 Cryptococcus neoformans strains and purified carbohydrates C. capsule were thus assayed the ELISA. Antigens all strains, galactoxylomannan, gave positive reaction, suggesting that galactoxylomannan contains an epitope(s) is cross-reactive galactomannan.
In 2002, the French Food Safety Agency drew attention to lack of information on prevalence human cryptosporidiosis in country. Two years later, ANOFEL Cryptosporidium National Network (ACNN) was set up provide public health authorities with data incidence and epidemiology France. Constituted a voluntary basis, ACNN includes 38 hospital parasitology laboratories (mainly university hospitals). Each laboratory is engaged notify new cases confirmed cryptosporidiosis, store specimens (e.g....
The efficacy and tolerance of a new amphotericin B lipid emulsion (AmB-IL) in which was diluted solution for parenteral nutrition (Intralipid) assessed fourteen episodes candidaemia occurring neutropenic patients. strains isolated were Candida krusei (nine cases), albicans (three parapsilosis (one case) lusitarnae case). An AmB-IL administered at mean dosage 1·18 mg/kg/day (range 0·73–1·55) 22 days 6–62). Flucytosine added to 12 patients (mean duration 10·6 days). Chills noted only 3/306...
Objectives: To investigate (1) the frequency of clinical resistance to oral polyenes or azole treatment for candidiasis, (2) resistant in vitro Candida strains, (3) relationship between susceptibilities species and vivo status HIV patients. Design: Prospective cross-sectional study. Setting: Tertiary care clinic at Bocage Hospital, Dijon, France. Patients: HIV-infected patients with without candidiasis. Interventions: Clinical examination, swab mycologic investigations. Main outcome...
In vivo MR spectroscopic imaging of the adrenal glands: distinction between adenomas and carcinomas larger than 15 mm based on lipid contentA Leroy-Willig, J Bittoun, JP Luton, A Louvel, JE Lefevre, Bonnin JC RoucayrolAudio Available | Share
We report here that modifications of the preanalytical steps matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) identification yeasts, with regard to original protocol provided by manufacturers, appear be efficient for reliable routine clinical yeast isolates in medical laboratories. Indeed, when one colony was sampled instead five and protein extraction modified, performance MALDI-TOF MS superior API ID 32C method (discrepancies were confirmed using...
C. albicans is a commensal yeast of the mucous membranes in healthy humans that can also cause disseminated candidiasis, mainly originating from digestive tract, vulnerable patients. It necessary to understand cellular and molecular mechanisms interaction with enterocytes better basis commensalism pathogenicity improve management candidiasis. In this study, we investigated kinetics tight junction (TJ) formation parallel invasion into Caco-2 intestinal cell line. Using invasiveness assays on...
The Platelia Candida-specific antigen and antibody assays (Bio-Rad Laboratories) were used to test serial serum samples from seven neutropenic adult patients with hematological malignancies who had developed systemic Candida tropicalis infections. diagnosis of candidiasis was based on a positive blood culture (all patients) the isolation C. normally sterile site (six patients). All received early antifungal therapy amphotericin B and/or an azole derivative successful outcomes. When combined...
ABSTRACT Candida albicans is a commensal dimorphic yeast of the digestive tract that causes hematogenously disseminated infections in immunocompromised individuals. Endogenous invasive candidiasis develops from C. adhering to intestinal epithelium. Adherence mediated by cell wall surface, domain composed essentially mannopyranosyl residues bound proteins, N-linked moiety which comprises sequences α-1,2- and β-1,2-linked mannose residues. mannosides are also associated with glycolipid,...
Summary OBJECTIVE The vast majority of patients with Cushing's disease have a corticotroph adenoma, the selective removal which, through transsphenoidal route, has potential to offer definitive and complete cure. This study was designed compare diagnostic accuracy computerized tomography (CT) magnetic resonance imaging (MRI) identify presence, evaluate size, assess topographic characteristics pituitary adenomas. METHODS Forty‐two were included in this prospective study, whom 16 subsequently...
To define the pathogenicity of respiration-deficient mutants Candida glabrata, which present a reduced susceptibility to azoles, that are easily induced in vitro by exposure these drugs or ethidium bromide and may be selected vivo patients receiving fluconazole.Two wild-type isolates C. glabrata were compared with their respective fluconazole- bromide-induced petite mutants, regarding carbohydrate protein composition cell wall, as well surface physical properties, also adherence abilities...
Candida albicans is the most frequent yeast responsible for systemic infections in humans. These mainly originate from gastrointestinal tract where C. can invade gut epithelial barrier to gain access bloodstream. Along gut, pathogens use Microfold (M) cells as a portal of entry cross barrier. M are specialized located follicule-associated epithelium Peyer patches. In this study, we used scanning electron and fluorescence microscopy, adhesion invasion assays fungal mutants investigate...