A5100632306- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Alcohol Consumption and Health Effects
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Signaling Pathways in Disease
- Endoplasmic Reticulum Stress and Disease
- Colorectal Cancer Treatments and Studies
- Cancer-related molecular mechanisms research
- Genomics, phytochemicals, and oxidative stress
- Sulfur Compounds in Biology
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Platelet Disorders and Treatments
- Peroxisome Proliferator-Activated Receptors
- Autoimmune Bullous Skin Diseases
- Gastric Cancer Management and Outcomes
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Thyroid Cancer Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Natural Compounds in Disease Treatment
- Epigenetics and DNA Methylation
University of Wolverhampton
2015-2025
Hohai University
2024-2025
Yantaishan Hospital
2015-2024
Southern University of Science and Technology
2024
First Affiliated Hospital of Zhengzhou University
2019-2023
Intelligent Health (United Kingdom)
2023
Neusoft (China)
2023
Liaoning Cancer Hospital & Institute
2023
Dalian University of Technology
2023
University of California, Los Angeles
2022-2023
Previous studies indicate that disulfiram (DS), an anti-alcoholism drug, is cytotoxic to cancer cell lines and reverses anticancer drug resistance. Cancer stem cells (CSCs) are the major cause of chemoresistance leading failure chemotherapy. This study intended examine effect DS on breast (BCSCs).The BC BCSCs was determined by MTT, western blot, CSCs culture marker analysis.Disulfiram highly toxic in vitro a copper (Cu)-dependent manner. In Cu-containing medium (1 μM), IC(50) concentrations...
Abstract 5‐Fluorouracil (5‐FU) is the major chemotherapeutic component for colorectal cancer (CRC) and other types of solid tumours. Resistance cells to 5‐FU considered obstacle successful chemotherapy. NF‐κB a transcription factor. Cancer with high nuclear activity demonstrate robust chemo‐ radio‐resistance. We demonstrated that in CRC cell lines, DLD‐1 RKO WT , was significantly induced by concentration‐ time‐dependent manner. IκBα degradation promoted both translocation its DNA binding...
Triple-negative breast cancer (TNBC) has significantly worse prognosis. Acquired chemoresistance remains the major cause of therapeutic failure TNBC. In clinic, relapsed TNBC is commonly pan-resistant to various drugs with completely different resistant mechanisms. Investigation mechanisms and development new target pan-chemoresistance will potentially improve outcomes patients.In this study, 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT), combination index (CI)-isobologram, western...
Abstract Acute myeloid leukemia (AML) is a heterogeneous malignancy. Despite the advances in past decades, clinical outcomes of AML patients remain poor. Leukemia stem cells (LSCs) major cause recurrence even after aggressive treatment making, promoting development LSC-targeted agents an urgent need. Although antitumor activity disulfiram (DS), approved anti-alcoholism drug, has been demonstrated multiple types tumors including hematological malignancies such as AML, it remains unknown...
Primary immune thrombocytopenia (ITP) is an autoimmune heterogeneous disorder that characterized by decreased platelet count. Regulatory T (Treg) cells and helper type 17 (Th17) are two subtypes of CD4(+) (Th) cells. They play opposite roles in tolerance diseases, while they share a common differentiation pathway. The imbalance Treg/Th17 has been demonstrated several diseases. In this study, we aimed to investigate the ratio number Treg Th17 ITP patients evaluate clinical implications...
// Peng Liu 1 , Zhipeng Wang Sarah Brown Vinodh Kannappan Patricia Erebi Tawari Wenguo Jiang 2 Juan M. Irache 3 James Z. Tang Stephen Britland Angel L. Armesilla John Darling Xing 4 and Weiguang Research Institute in Healthcare Science, Faculty of Science & Engineering, University Wolverhampton, UK Cardiff University-Peking Cancer Institute, School Medicine, Henry Wellcome Building, Heath Park, Cardiff, Pharmacy, Navarra, Pamplona, Spain Shenyang Pharmaceutical University, Shenyang, China...
Disulfiram (DS), an anti-alcoholism drug, demonstrates strong antitumor activity in a copper (Cu)-dependent manner. This study investigates the cytotoxicity of DS/Cu complex lymphoid malignant cell lines vitro and vivo. Raji cells were subjected to different treatments thereafter MTT assay, flow cytometry used determine IC50 apoptotic status. We also tested acute lymphoblastic leukemia line Molt4 vitro. In vivo experiments performed demonstrate anticancer efficacy xenografted nude mice....
Disulfiram (DS), an anti-alcoholism drug, shows very strong cytotoxicity in many cancer types. However its clinical application treatment is limited by the short half-life bloodstream. In this study, we developed a poly lactic-co-glycolic acid (PLGA)-encapsulated DS protecting from degradation The newly DS-PLGA was characterized. has satisfactory encapsulation efficiency, drug-loading content and controlled release rate vitro. PLGA extended of shorter than 2minutes to 7hours serum....
The anticancer activity of disulfiram (DS) is copper(ii) (Cu)-dependent. This study investigated the mechanisms DS/Cu using in vitro cytotoxicity and metabolic kinetic analysis. Our indicates that targets cancer cells by combination two types actions: (1) instant killing executed reaction generated reactive oxygen species; (2) delayed introduced end product, DDC-Cu. Nanoencapsulation DS might shed light on repositioning into treatment.
Disulfiram (DS), an anti-alcoholism medicine, shows strong anti-cancer activity in the laboratory, but application clinics for therapy has been limited by its prompt metabolism. Conventional liposomes have shown ability to protect DS. Therefore, aim of this study is develop PEGylated DS enhanced bio-stability and prolonged circulation. were prepared using ethanol-based proliposome methods. Various ratios phospholipids, namely: hydrogenated soya phosphatidylcholine (HSPC) or dipalmitoyl...
Abstract Gene expression was analyzed in five pairs of 5-fluorouracil (5-FU) resistant and parental cancer cell lines on DNA microarrays. In unsupervised analysis, a prediction rule built from the profiles 29 genes, 5-FU sensitivity class predicted with 100% accuracy high predictive strength. supervised analysis key pathways, 91 genes associated phenotype segregated samples accordingly hierarchical analysis. Key involved activation were significantly down-regulated (thymidine kinase,...
Leukemia stem cells (LSCs) are considered to be the main reason for relapse and also regarded as a major hurdle success of acute myeloid leukemia chemotherapy. Thus, new drugs targeting LSCs urgently needed. Triptolide (TPL) is cytotoxic LSCs. Low dose TPL enhances cytotoxicity idarubicin (IDA) in In this study, ability induce apoptosis leukemic cell (LSC)-like derived from line KG1a was investigated. LSC-like sorted were subjected cycle analysis different treatments, then followed by vitro...
Objective— Vascular endothelial growth factor (VEGF) has been identified as a crucial regulator of physiological and pathological angiogenesis. Among the intracellular signaling pathways triggered by VEGF, activation calcineurin/nuclear activated T cells (NFAT) axis emerged critical mediator angiogenic processes. We others previously reported novel role for plasma membrane calcium ATPase (PMCA) an endogenous inhibitor calcineurin/NFAT pathway, via interaction with calcineurin, in...
Disulfiram (DS), an antialcoholism medicine, demonstrated strong anticancer activity in the laboratory but did not show promising results clinical trials. The of DS is copper dependent. reaction and generates reactive oxygen species (ROS). After oral administration clinic, enriched quickly metabolised liver. associated change chemical structure may make metabolites lose its copper-chelating ability disable their activity. still largely unknown. Elucidation relationship between key will...
Abstract Anion exchanger 1 (AE1, band 3) is a major membrane protein of red blood cells and plays key role in acid-base homeostasis, urine acidification, cell shape regulation, removal carbon dioxide during respiration. Though structures the transmembrane domain (TMD) three SLC4 transporters, including AE1, have been resolved previously their outward-facing (OF) state, no mammalian structure has reported inward-facing (IF) conformation. Here we present cryoEM full-length bovine AE1 with its...
Abstract Glioblastoma stem cell (GSC) is the major cause of glioblastoma multiforme (GBM) chemotherapy failure. Hypoxia one determinants GSC. NF-κB plays a pivotal link between hypoxia and cancer cells (CSCs). Disulfiram, an antialcoholism drug, has very strong NF-κB–inhibiting anti-CSC activity. In this study, in vitro anti-GSC activity disulfiram vivo anti-GBM efficacy poly lactic–co-glycolic acid nanoparticle-encapsulated (DS-PLGA) were examined. We attempt to elucidate molecular network...