- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme function and inhibition
- Drug Transport and Resistance Mechanisms
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Immune Response and Inflammation
- Analytical Chemistry and Chromatography
- Bacterial Genetics and Biotechnology
- Phenothiazines and Benzothiazines Synthesis and Activities
- Cholinesterase and Neurodegenerative Diseases
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Polyamine Metabolism and Applications
- Microbial Natural Products and Biosynthesis
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
Gwangju Institute of Science and Technology
2016-2025
Spark Therapeutics (United States)
2022-2025
Ark Therapeutic (United States)
2025
Wenzhou Medical University
2016-2023
Teva Pharmaceuticals (United States)
2018-2021
China Agricultural University
2019
Bristol-Myers Squibb (United States)
2009-2017
Wenzhou University
2016
Bristol-Myers Squibb (Germany)
2008-2014
Purdue University West Lafayette
2012
For characterization of sequence and posttranslational modifications, molecular fragment ion mass data from ionizing dissociating a protein in the spectrometer are far more specific than masses peptides protein's digestion. We extend ∼500-residue, ∼50-kilodalton (kD) dissociation limitation this top-down methodology by using electrospray additives, heated vaporization, separate noncovalent covalent bond dissociation. This process can cleave 287 interresidue bonds termini 1314-residue...
Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces extensive immune responses that can lead to fatal septic shock syndrome. The core receptors recognizing lipopolysaccharide are CD14, TLR4, and MD-2. CD14 binds presents it TLR4/MD-2 complex, which initiates intracellular signaling. In addition lipopolysaccharide, is capable a few other microbial cellular products. Here, we present first crystal structure 2.5 angstroms resolution. A large hydrophobic pocket was found on...
Mammalian sterile 20-like kinase 1 (Mst1) is activated by both caspase-mediated cleavage and phosphorylation in response to apoptotic stimuli, including Fas ligation. Here, we examined the possible role of tumor suppressor RASSF1A Mst1 activation Mst1-mediated apoptosis induced death receptor signaling. Immunoprecipitation immunofluorescence analyses revealed that was associated with cultured mammalian cells, proteins colocalizing microtubules throughout cell cycle. Whereas purified...
Host cell proteins (HCPs) constitute a major group of impurities for biologic drugs produced using culture technology. HCPs are required to be closely monitored and adequately removed in the downstream process. However, complex mixture with significantly diverse molecular immunological properties. An overall understanding composition changes their properties upon upstream harvest process conditions can greatly facilitate design. This article describes use comparative proteomic profiling...
N6A-methyladenosine (m6A) post-transcriptional modification, the most abundant internal RNA is catalyzed by METTL3-14 methyltransferase complex. Recently, attention has been drawn to complex regarding its significant roles in pathogenesis of acute myeloid leukemia (AML), attracting potential novel therapeutic targets for disease. Herein, we report identification and characterization eltrombopag as a selective allosteric inhibitor Eltrombopag exhibited inhibitory activity active catalytic...
Increasing bacterial resistance to antibiotics with conventional targets has focused attention on unconventional targets. One promising candidate, the acetyl-CoA carboxylase (ACC) inhibitor andrimid, is a potent, broad-spectrum antibiotic high selectivity for prokaryotic ACC. Here, we report use of DNA-based approach clone andrimid biosynthetic gene cluster from Pantoea agglomerans, yielding cosmid that confers robust production Escherichia coli. This encodes hybrid nonribosomal...
Abstract Chronic hepatitis B virus (HBV) infection can cause cirrhosis and hepatocellular carcinoma is therefore a serious public health problem. Infected patients are currently treated with nucleoside/nucleotide analogs interferon α, but this approach not curative. Here, we screen 978 FDA-approved compounds for their ability to inhibit HBV replication in HBV-expressing HepG2.2.15 cells. We find that ciclopirox, synthetic antifungal agent, strongly inhibits cells mice by blocking capsid...
Recombinant adeno-associated viral vector (rAAV) mediated gene therapy is gaining traction in treating genetic disorders. Current rAAV production systems yield a mixture of capsids largely devoid the transgene (empty capsid) compared with desired therapeutic product (full capsid). Anion exchange chromatography (AEX) an attractive method for separating empty and full AAV because its scalability. Resin types buffer composition are key considerations AEX must support capsid stability to be...
Host cell proteins (HCPs) are process-related impurities of biopharmaceuticals that remain at trace levels despite multiple stages downstream purification. Currently, there is interest in implementing LC-MS biopharmaceutical HCP profiling alongside conventional ELISA, because individual species can be identified and quantitated. Conventional data dependent hampered by the low concentration HCP-derived peptides, which 5-6 orders magnitude less abundant than biopharmaceutical-derived peptides....
m6A RNA methyltransferase (METTL3-14) catalyzes the methylation of adenosine in mRNA and plays important roles functions, it has been implicated progression multiple cancers, including acute myeloid leukemia (AML). In this study, we describe discovery first allosteric inhibitor METTL3-14 complex based on structure–activity relationship (SAR) optimization studies hit compound, 4-[2-[5-chloro-1-(diphenylmethyl)-2-methyl-1H-indol-3-yl]-ethoxy]benzoic acid (CDIBA). Compound 43n was optimized...
An antibacterial and pro-osteogenic coaxially electrospun nanofiber guided bone regeneration (GBR) membrane was fabricated to satisfy the complicated phased requirements of GBR process.In this study, we synthesize dual-functional membranes by encapsulating parathyroid hormone (PTH) in core layer magnesium oxide nanoparticles (MgONPs) shell (MgONPs-PCL/PTH-PCL). Herein, physicochemical characterization MgONPs-PCL/PTH-PCL, release rates MgONPs PTH, efficiency new were evaluated. Furthermore,...
Small-molecule chemistry and biochemical techniques have been used to determine the structure mode of function pantocin A (see structure), a naturally occurring antibiotic. Pantocin functions as an inhibitor histidine biosynthesis, shown by its ability inhibit action L-histidinol phosphate aminotransferase, which converts imidazole acetol in Escherichia coli. The epiphytic bacterium Erwinia amylovora causes fire blight, devastating disease rosaceous plants such apple pear.1 closely related...
ABSTRACT The thioquinolobactin siderophore from Pseudomonas fluorescens ATCC 17400 utilizes a variation of the sulfur transfer chemistry found in thiamine and molydobterin biosynthesis. A JAMM motif protein cleaves C-terminal amino acid residues following diglycine moiety on small carrier protein, modified C terminus is activated sulfurylated, forming thiocarboxylate.
ABSTRACT In this work, a proof of concept elastin‐like polypeptide‐Z domain fusion (ELP‐Z) based affinity precipitation process is developed for monoclonal antibody (mAb) purification from industrial harvest feeds. Greater than 99% mAb recoveries are obtained during the initial binding step both pure and Great 90% overall yields also subsequent elution with no measurable aggregation. The shown to result in more 2 logs host cell protein (HCP) 4 DNA clearance feed. While yield HCP was...
Songwon Kim, Sang Soo Lee, Jun Gyou Park, Ji Won Seulgi Ju, Seung Hun Choi, Subin Na Jin Semi Hong, Young Kang, and Mi Sun Jin. Mol. Cells 2022;45:575-87. https://doi.org/10.14348/molcells.2022.0040
While adeno-associated virus is a leading vector for gene therapy, significant gaps remain in understanding AAV degradation and stability. In this work, we study the of an engineered serotype at physiological pH ionic strength. Viral particles varying fractions encapsulated DNA were incubated between 30 60 °C, with changes molecular weight measured by total light scattering intensity 90° over time. Mostly full vectors demonstrated rapid decrease corresponding to release capsid DNA, followed...
Drosophila I’m Not Dead Yet (INDY) functions as a transporter for citrate, key metabolite in the citric acid cycle, across plasma membrane. Partial deficiency of INDY extends lifespan, akin to effects caloric restriction. In this work, we use cryo-electron microscopy determine structures presence and absence citrate complex with well-known inhibitor 4,4′-diisothiocyano-2,2′-disulfonic stilbene (DIDS) at resolutions ranging from 2.7 3.6 Å. Together functional data obtained vitro, reveal H +...