A5011090537- Antifungal resistance and susceptibility
- Streptococcal Infections and Treatments
- Bacterial Infections and Vaccines
- Fungal Infections and Studies
- Pneumonia and Respiratory Infections
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Mycology
- Mycobacterium research and diagnosis
- Otolaryngology and Infectious Diseases
- Plant Pathogens and Fungal Diseases
- Clostridium difficile and Clostridium perfringens research
- Immune Response and Inflammation
- Respiratory viral infections research
- Nail Diseases and Treatments
- Cystic Fibrosis Research Advances
- Tuberculosis Research and Epidemiology
- Respiratory and Cough-Related Research
- Nematode management and characterization studies
- Infections and bacterial resistance
- Bacterial Genetics and Biotechnology
- European and International Law Studies
University of Oslo
2013-2025
Oslo University Hospital
2010-2020
Akershus University Hospital
1984-2008
Bærum Sykehus
2003-2006
National Hospital
1992-2004
Med-Storm Innovation (Norway)
2002
We studied prospectively the quantitative relation of circulating endotoxin (lipooligosaccharides [LOSs]) and development multiple organ failure death in 45 consecutively admitted patients with bacteriologically verified systemic meningococcal disease (SMD). A plasma LOS level greater than 700 ng/L correlated severe septic shock (P less .0001), adult respiratory distress syndrome = .0035), a pathologically elevated serum creatinine or as consequence .0002). Initial levels 25, 25-700,...
Background Inappropriate cross talk between mammals and their gut microbiota may trigger intestinal inflammation drive extra-intestinal immune-mediated diseases. Epithelial cells constitute the interface host tissue, regulate responses to commensal enteric bacteria. Gnotobiotic animals represent a powerful approach study bacterial-host interaction but are not readily accessible wide scientific community. We aimed at refining protocol that in robust manner would deplete cultivable of...
To investigate azole resistance in clinical Aspergillus isolates, we conducted prospective multicenter international surveillance. A total of 3,788 isolates were screened 22 centers from 19 countries. Azole-resistant A. fumigatus was more frequently found (3.2% prevalence) than previously acknowledged, causing resistant invasive and noninvasive aspergillosis severely compromising use azoles.
Competence for genetic transformation in certain species of streptococci has been known many years to be induced by a secreted protease-sensitive pheromone, referred as the competence factor or activator, which acts quorum-sensing signal co-ordinate expression late genes. We recently reported identification pheromone Streptococcus pneumoniae strain Rx small unmodified peptide, was termed competence-stimulating peptide (CSP). By identifying gene (comC) encoding CSP we were able show that it...
To map the incidence of natural competence in genus Streptococcus, we used PCR to screen a number streptococcal strains for presence recently identified regulation operon, containing comC, -D, and -E genes. This approach established that operon is present belonging S. mitis anginosus groups, but it was not detected other examined. Competence induced pneumoniae gordonii by strain-specific peptide pheromones, competence-stimulating peptides (CSPs). With its unique primary structure, each CSP...
ABSTRACT Infections due to coagulase-negative staphylococci (CoNS) most frequently occur after the implantation of medical devices and are attributed biofilm-forming potential CoNS. Staphylococcus haemolyticus is second isolated CoNS from patients with hospital-acquired infections. There only limited knowledge nature S. biofilms. The aim this study was characterize biofilm formation. We analyzed capacities 72 clinical isolates. A detachment assay NaIO 4 , proteinase K, or DNase used...
Although Clinical and Laboratory Standards Institute (CLSI) clinical breakpoints (CBPs) are available for interpreting echinocandin MICs Candida spp., epidemiologic cutoff values (ECVs) based on collective MIC data from multiple laboratories have not been defined. While collating CLSI caspofungin 145 to 11,550 isolates 17 (Brazil, Canada, Europe, Mexico, Peru, the United States), we observed an extraordinary amount of modal variability (wide ranges) among as well truncated bimodal...
ABSTRACT In order to determine the occurrence, distribution, and significance of mold species in groundwater- surface water-derived drinking water Norway, molds isolated from 273 samples were identified. Samples raw water, treated private homes hospital installations analyzed by incubation 100-ml membrane-filtered on dichloran-18% glycerol agar. The total count (number CFU per 100 ml) fungal diversity within each sample determined. identification was based morphological molecular methods....
ABSTRACT A long-term, nationwide prospective candidemia study has been ongoing in Norway since 1991. All medical microbiological laboratories the country have participated. During period 1991 to 2003 a total of 1,393 episodes occurred 1,348 patients. The incidence per 100,000 inhabitants increased from approximately 2 early 1990s 3 2001 2003. average annual incidences varied markedly between age groups. was high patients aged <1 year and ≥70 years. In ≥80 years age, during last an 6.5...
Natural competence in Streptococcus pneumoniae is regulated by a quorum‐sensing mechanism consisting of competence‐stimulating peptide (CSP), its dedicated secretion apparatus (ComAB), histidine kinase receptor (ComD) and response regulator (ComE). In this report, we show that ComE DNA‐binding protein acts autocatalytically binding to region own promoter. Two additional sites were identified the pneumococcal genome, one promoter comAB other upstream an ABC transporter unknown function. A...
Variation in nine enzymes 152 isolates of Neisseria meningitidis from Norway (118 blood or cerebrospinal fluid patients with systemic disease and 34 the pharynx healthy carriers) was analysed by starch-gel electrophoresis. All were polymorphic number allozymes (electromorphs) identified per locus ranged 3 to 12, a mean 6.1. Among isolates, 55 unique combinations electromorphs (electrophoretic types, ETs) distinguished. Twenty ETs represented among carrier 37 isolates; hence, only two found...
ABSTRACT There has been an increase in data suggesting that besides air, hospital water is a potential source of transmission filamentous fungi, and particular Aspergillus fumigatus . Molecular characterization environmental clinical A. isolates, collected prospectively during 18-month period, was performed to establish if waterborne fungi play role the pathogenesis invasive aspergillosis. Isolates recovered from ( n = 54) air 21) at various locations inside outside 15 patients with proven,...
Innate and adaptive mucosal defense mechanisms ensure a homeostatic relationship with the large complex mutualistic gut microbiota. Dimeric IgA pentameric IgM are transported across intestinal epithelium via epithelial polymeric Ig receptor (pIgR) provide significant portion of first line natural or antibody-mediated immune mucosa. We found that colonic cells from pIgR KO mice differentially expressed (more than twofold change) more 200 genes compared WT mice, upregulated expression...
ABSTRACT The present study was performed to assess the interlaboratory reproducibility of molecular detection and identification species Zygomycetes from formalin-fixed paraffin-embedded kidney brain tissues obtained experimentally infected mice. Animals were with one five ( Rhizopus oryzae , microsporus Lichtheimia corymbifera Rhizomucor pusillus Mucor circinelloides ). Samples 1, 10, or 30 slide cuts prepared each paraffin block, sample identities blinded for analysis, samples mailed seven...
Rapid development within the field of massive parallel sequencing (MPS) is about to bring this technology reach for diagnostic microbiology laboratories. We wanted explore its potential improving diagnosis and understanding polymicrobial infections, using bacterial brain abscesses as an example. conducted a prospective nationwide study on abscesses. Fifty-two surgical samples were included over 2-year period. The categorized either spontaneous intracerebral, subdural, or postoperative....
In order to determine the occurrence of filamentous fungi in public drinking water systems Norway, from 14 supply networks all over country was sampled and analysed. Networks with both ground surface sources were included this study.During a one-year period, 273 samples collected. Frequencies raw water, treated home hospital installations determined on basis incubation 100 ml membrane-filtered dichloran 18% glycerol agar media. Filamentous recovered 62% samples. 42.3% positive for mould...
Type IV pili (Tfp), which have been studied extensively in a few Gram-negative species, are the paradigm of group widespread and functionally versatile nano-machines. Here, we performed most detailed molecular characterisation Tfp Gram-positive bacterium. We demonstrate that naturally competent Streptococcus sanguinis produces retractable Tfp, like their counterparts can generate hundreds piconewton tensile force promote intense surface-associated motility. power 'train-like' directional...