A5023468002- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Plant Virus Research Studies
- DNA Repair Mechanisms
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- Crop Yield and Soil Fertility
- RNA Research and Splicing
- Advanced X-ray and CT Imaging
- Plant Reproductive Biology
- Genetics and Plant Breeding
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Telomeres, Telomerase, and Senescence
- Plant Genetic and Mutation Studies
- RNA and protein synthesis mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Hops Chemistry and Applications
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
Florida State University
2016-2025
Tallahassee Orthopedic Clinic
2013
University of Missouri
2008-2009
University of Minnesota
2008
United States Department of Agriculture
2008
Agricultural Research Service
2008
Oregon State University
2008
Illinois State University
2008
Carleton College
2008
University of Florida
2008
Significance The maize genome, similar to those of most plant genomes, is 98% noncoding. Much the remainder a vast desert repeats that remain repressed throughout cell cycle. orchestrates its complex activities by restricting access functional regions with an open chromatin configuration. Here, we identify small portion (<1%) genome residing in chromatin. We demonstrate predicts molecular phenotypes such as gene expression and recombination. Furthermore, show genetic variation within...
The maize W22 inbred has served as a platform for genetics since the mid twentieth century. To streamline genome analyses, we have sequenced and de novo assembled reference using short-read sequencing technologies. We show that significant structural heterogeneity exists in comparison to B73 at multiple scales, from transposon composition copy number variation single-nucleotide polymorphisms. generation of this enables accurate placement thousands Mutator (Mu) Dissociation (Ds) transposable...
We have analyzed the progressive changes in spatial distribution of telomeres during meiosis using three-dimensional, high resolution fluorescence microscopy. Fixed meiotic cells maize (Zea mays L.) were subjected to situ hybridization under conditions that preserved chromosome structure, allowing identification stage-dependent telomere arrangements. found nuclei at last somatic prophase before exhibit a nonrandom, polarized organization resulting loose grouping telomeres. Quantitative...
ABSTRACT To improve knowledge of the prerequisites for meiotic chromosome segregation in higher eukaryotes, we analyzed spatial distribution a pair homologs before and during early prophase. Three-dimensional images fluorescence situ hybridization (FISH) were used to localize single diploid nuclei chromosome-addition line oat, oat-maize9b. The system provided robust assay pairing based on cytological colocalization FISH signals. Using triple labeling scheme simultaneous imaging chromatin,...
Although synthesis of the cytosolic maize albumin b-32 had been shown to be controlled by Opaque-2 regulatory locus, its function was unknown. We show here that is a member large and widely distributed class toxic plant proteins with ribosome-inactivating activity. These (RIPs) are RNA N-glycosidases remove single base from conserved 28S rRNA loop required for elongation factor 1 alpha binding. Cell-free in vitro translation extracts were used both wheat ribosomes resistant molar excesses...
The eukaryotic genome is organized into nucleosomes, the fundamental units of chromatin. positions nucleosomes on DNA regulate protein-DNA interactions and in turn influence DNA-templated events. Despite increasing number genome-wide maps nucleosome position, how global changes gene expression relate to position poorly understood. We show that occupancy mapping experiments maize (Zea mays), particular genomic regions are highly susceptible variation introduced by differences extent which...
Abstract Background The nuclear envelope that separates the contents of nucleus from cytoplasm provides a surface for chromatin attachment and organization cortical nucleoplasm. Proteins associated with it have been well characterized in many eukaryotes but not plants. SUN (Sad1p/Unc-84) domain proteins reside inner membrane function other to form physical link between nucleoskeleton cytoskeleton. These bridges transfer forces across are increasingly recognized play roles positioning,...
The G-quadruplex (G4) elements comprise a class of nucleic acid structures formed by stacking guanine base quartets in quadruple helix. This G4 DNA can form within or across single-stranded molecules and is mutually exclusive with duplex B-form DNA. reversibility structural diversity G4s make them highly versatile genetic structures, as demonstrated their roles various functions including telomere metabolism, genome maintenance, immunoglobulin gene diversification, transcription,...
The functional genome of agronomically important plant species remains largely unexplored, yet presents a virtually untapped resource for targeted crop improvement. Functional elements regulatory DNA revealed through profiles chromatin accessibility can be harnessed fine-tuning gene expression to optimal phenotypes in specific environments.Here, we investigate the non-coding space maize (Zea mays) during early reproductive development pollen- and grain-bearing inflorescences. Using an assay...
Elucidating the transcriptional regulatory networks that underlie growth and development requires robust ways to define complete set of transcription factor (TF) binding sites. Although TF-binding sites are known be generally located within accessible chromatin regions (ACRs), pinpointing these DNA elements globally remains challenging. Current approaches primarily identify for a single TF (e.g. ChIP-seq), or detect ACRs but lack resolution consistently DNAse-seq, ATAC-seq). To address this...
Abstract Polyploidy is a prominent mechanism of plant speciation and adaptation, yet the mechanistic understandings duplicated gene regulation remain elusive. Chromatin structure dynamics are suggested to govern regulatory control. Here, we characterized genome-wide nucleosome organization chromatin accessibility in allotetraploid cotton, Gossypium hirsutum (AADD, 2n = 4X 52), relative its two diploid parents (AA or DD genome) their synthetic hybrid (AD), using DNS-seq. The larger A-genome...
The essential Yin Yang-1 gene (YY1) encodes a ubiquitous, conserved, multifunctional zinc-finger transcription factor in animals. YY1 protein regulates initiation, activation, or repression of from variety genes required for cell growth, development, differentiation, tumor suppression, as well some retroviruses and DNA viruses. Among the specific functions attributed to is role cell-cycle-specific upregulation replication-dependent histone genes. binds alpha element, regulatory sequence...
Abstract The maize (Zea mays L.) floury-2 (fl2) mutation is associated with a general decrease in storage protein synthesis, altered body morphology, and the synthesis of novel 24-kD α--zein protein. Unlike proteins normal kernels majority fl2 kernels, contains signal peptide that would normally be removed during processing. expected processing site this reveals putative alaine-&gt;valine (Ala-&gt;Val) not found at other junctions between sequences mature proteins. To investigate...
The nuclear envelope (NE) plays an essential role in meiotic telomere behavior and links the cytoplasm nucleoplasm during homologous chromosome pairing recombination many eukaryotic species. Resident NE proteins including SUN (Sad-1/UNC-84) KASH (Klarsicht/ANC-1/Syne-homology) domain are known to interact forming Linker of Nucleoskeleton Cytoskeleton (LINC) complex that connects chromatin cytoskeleton. To investigate possible cross-kingdom conservation protein functions plant meiosis, we...
Abstract We screened maize (Zea mays) cDNAs for sequences similar to the single myb-like DNA-binding domain of known telomeric complex proteins. identified, cloned, and sequenced five full-length representing a novel gene family, we describe analysis one them, Single myb histone 1 (Smh1). The Smh1 encodes small, basic protein with unique triple motif structure (a) an N-terminal SANT/myb-like homeodomain-like superfamily 3-helical-bundle-fold proteins, (b) central region homology conserved H1...
Summary The development of a cytogenetic map for maize ( Zea mays L.) is shown to be feasible by means combination resources from sorghum and oat that overcome limitations single‐copy gene detection. A chromosome‐addition line oat, OMAd9.2, provided clear images optically isolated pachytene chromosomes through chromosome spread painting technique. direct labeled oligonucleotide fluorescence in situ hybridization (FISH) probe MCCY specifically stained the centromere. arm ratio (long/short) 9...
Spatiotemporal patterns of DNA replication have been described for yeast and many types cultured animal cells, frequently after cell cycle arrest to aid in synchronization. However, nuclei from plants or naturally developing organs remain largely uncharacterized. Here we report findings 3D quantitative analysis endoreduplication pulse-labeled maize root tips. In both early middle S phase nuclei, flow-sorted on the basis content, replicative labeling was widely distributed across euchromatic...
The linker of nucleoskeleton and cytoskeleton (LINC) complex is an essential multi-protein structure spanning the eukaryotic nuclear envelope. LINC functions to maintain architecture, positioning, mobility, along with specialized in meiotic prophase chromosome segregation. Members were recently identified maize, important scientific agricultural grass species. Here we characterized
<title>Abstract</title> Genome replication is temporally regulated during S phase, with specific genomic regions replicating at defined times in a process known as Replication Timing (RT). Based on 3D cytology nuclei, we previously proposed “mini-domain chromatin fiber RT model” for maize euchromatin that suggested it subdivided into early-S and middle-S compartments distinguished by condensation RT. However, whether this compartmentalization reflects general nuclear architecture persists...
ABSTRACT Transcriptional signaling during hypoxic stress is primarily governed by hypoxia-inducible transcription factors (HIFs), which bind to hypoxia response elements (HREs) in gene regulatory regions. However, only a small proportion (∼1%) of known HREs are occupied HIFs hypoxia, suggesting the involvement additional pathways. To address this gap, we utilized MOA-seq, an MNase-based assay that enables genome-wide, high-resolution (<30 bp) identification factor (TF) occupancy...