A5103156280- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- Gastric Cancer Management and Outcomes
- Bone Metabolism and Diseases
- Metastasis and carcinoma case studies
- Osteoarthritis Treatment and Mechanisms
- Circular RNAs in diseases
- Esophageal and GI Pathology
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Dermatologic Treatments and Research
- Mesenchymal stem cell research
- Metabolism, Diabetes, and Cancer
- Cell Adhesion Molecules Research
- Bone and Dental Protein Studies
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Bone health and treatments
- Diabetes Treatment and Management
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Graphene and Nanomaterials Applications
XinHua Hospital
2017-2024
Shanghai Jiao Tong University
2015-2024
Peking University
2024
Peking University First Hospital
2024
University of Science and Technology Beijing
2024
Fujian Medical University
2021-2023
Fujian Provincial Hospital
2021-2023
Northeast Electric Power University
2022
Dimensional Imaging (United Kingdom)
2022
Weatherford College
2022
Abstract The incomplete understanding of aberrant neovascularization, which contributes to osteoarthritis suggests that additional modulators have yet be identified. Our objective was identify the role Leucine-rich-alpha-2-glycoprotein1 (LRG1), a new regulator pathogenic angiogenesis, in progression and develop effective treatment strategies. In this study, immunohistochemistry showed LRG1 increased subchondral bone articular cartilage anterior cruciate ligament transection (ACLT) mice....
Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is essential for the formation, and emerging evidence shows that long non-coding RNAs (lncRNAs) play important roles in hBMSC osteogenic differentiation. MALAT1 often regarded as a tumor-related lncRNA, but its function cell remains to be defined. In this study, we aimed investigate whether regulates Osterix (Osx) expression by sponging miR-143 promote Firstly, found was much lower hBMSCs from...
Abstract Cartilage dyshomeostasis contributes to osteoarthritis (OA) pathogenesis, and tumor necrosis factor (TNF)- α has critical role in this process by driving inflammatory cascades cartilage degradation. However, the negative regulation of TNF- -mediated signaling remains undefined. Here we demonstrate crucial miR-145 modulation matrix MicroRNA (miRNA) expression profiles -stimulated chondrocytes showed that was rapidly downregulated . Moreover, directly repressed p65 negatively...
Abstract Hypertrophic scar (HS) formation is a skin fibroproliferative disease that occurs following cutaneous injury, leading to functional and cosmetic impairment. To date, few therapeutic treatments exhibit satisfactory outcomes. The mechanical force has been shown be key regulator of HS formation, but the underlying mechanism not completely understood. Piezo1 channel identified as novel mechanically activated cation (MAC) reportedly capable regulating force-mediated cellular biological...
Bone morphogenetic protein 2 (BMP2) has been used to induce bone regeneration by promoting osteogenic differentiation of marrow-derived mesenchymal stem cells (MSCs). However, its effect is attenuated in osteoporotic conditions unknown mechanisms. In this study, we investigated the molecular mechanisms reduced BMP2 conditions. By interrogating microarray data from osteoporosis patients, revealed an upregulation epigenetic modifying lysine (K)-specific demethylase 5A (KDM5A) and decreased...
Abstract Osteoarthritis (OA), characterized by insufficient extracellular matrix synthesis and cartilage degeneration, is known as an incurable disease because its pathogenesis poorly elucidated. Thus far, limited information available regarding the pathophysiological role of microRNAs (miRNAs) in OA. In this study, we investigated specific function miR-146a OA pathophysiology using mouse models. We found that articular degeneration knockout (KO) mice was alleviated compared with wild-type...
Small extracellular vesicles (sEVs) derived from M2 microglia (M2-microglia-derived small [M2-sEVs]) contribute to central nervous system repair, although the underlying mechanism remains unknown. In this study, we aimed identify through which microRNA-124 (miR-124) carried in sEVs promotes neural stem cell (NSC) proliferation and neuronal differentiation ischemic mouse brain.M2-sEVs with or without miR-124 knockdown were injected intravenously for 7 consecutive days after transient middle...
Tantalum, as a potential metallic implant biomaterial, is attracting more and attention because of its excellent anticorrosion biocompatibility. However, significantly high elastic modulus large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants. In this study, porous tantalum coatings were first successfully fabricated on titanium substrates by vacuum plasma spraying (VPS), which would exert the biocompatibility alleviate tissue. We evaluated...
Altered expression of circular RNAs (circRNAs) has been identified in various human diseases. In this study, we investigated whether circRNAs function as competing endogenous to regulate the pathological process temporomandibular joint osteoarthritis (TMJOA). High-throughput sequencing mRNA (RNA seq) was performed detect TMJOA and control synovial tissues isolated from humans. The differentially upregulated circGCN1L1 (hsa_circ_0000448) synoviocyte validated vitro vivo. Here demonstrate...
N6-methyladenosine (m6A) is required for differentiation of human bone marrow mesenchymal stem cells (hBMSCs). However, its intrinsic mechanisms are largely unknown. To identify the possible role m6A binding protein YTHDF1 in hBMSCs osteogenesis vivo, we constructed Ythdf1 KO mice and showed that depletion would result decreased mass vivo. Both deletion mouse BMSCs shRNA-mediated knockdown prevented osteogenic vitro. Using methylated RNA immunoprecipitation (Me-RIP) sequencing...
Osteoarthritis (OA) is the most common degenerative disease of synovial joint. The membrane responsible for inflammatory reaction leading to secretion macrophage-derived pro-inflammatory cytokines, such as IL-6. Suppressing IL-6 over-expression in fibroblasts (SF) a promising method prevent OA development and progression, which prerequisite elucidation molecular mechanisms underlying SF. Currently, there are few reports concerning epigenetic modifications In present study, we attempted...
Adipose-derived stem cells (ADSCs) are a subset of mesenchymal (MSCs), which have promised vast therapeutic potential in tissue regeneration. Recent studies demonstrated that combining with mechanical stretch may strengthen the efficacy regenerative therapies. However, exact influences on MSCs still remain inconclusive. In this study, human ADSCs (hADSCs) were applied cyclic stimulation under an vitro stretching model for designated duration. We found significantly promoted proliferation,...
Background: Skeletal unloading usually induces severe disuse osteoporosis (DOP), which often occurs in patients subjected to prolonged immobility or spaceflight astronauts. Increasing evidence suggests that exosomes are important mediators maintaining the balance between bone formation and resorption. We hypothesized play an role maintenance of homeostasis through intercellular communication marrow mesenchymal stem cells (BMSCs) osteoclasts under mechanical loading.Methods: Cells were...
Lymph node metastasis (LNM) is common in papillary thyroid cancer (PTC), and an indicator of recurrence. The detailed molecular mechanism LNM PTC has not been well described. This study aimed to investigate the role fibronectin 1 its clinical relevance. expression was confirmed tissues cell lines. A correlation analysis conducted a receiver-operating characteristic curve obtained. effect on proliferation lines performed using colony-formation assay Cell Counting Kit 8. Cell-cycle with...
Postmenopausal osteoporosis is a disease characterized by excessive osteoclastic bone resorption. Some anti-diabetic drugs were demonstrated for anti-osteoclastic bone-loss effects. In this study, we tested the skeletal effects of chronic administration sitagliptin, dipeptidyl peptidase IV (DPP IV) inhibitor that increasingly used type 2 diabetes treatments, in an estrogen deficiency-induced and elucidated associated mechanisms. We found sitagliptin effectively prevented ovariectomy-induced...
Macrophages (Mφs) can be used as a part of cell‐based cancer immunotherapy. However, they may hampered by failure to effectively and stably regulate their polarization state enhance tumoricidal effects. In this work, mechanical stretch (MS), biology‐free modulatory method, was shown M1 By using an in vitro Flexcell Tension system, we found that murine Mφ RAW264.7 cells showed higher polarization‐related mRNA expression cytokine release after MS. Further molecular analyses focal adhesion...
Although the mechanisms of Tumor necrosis factor alpha (TNF-α) on facilitating osteoclast differentiation and bone resorption is well known, behind suppression osteoblast from mesenchymal stem cells (MSCs) are still poorly understood. In this study, we observed a negative correlation between TNF-α levels expression special AT-rich sequence-binding protein 2 (SATB2), critical osteoblastogenesis transcription factor, in ovariectomy (OVX)-induced loss IL-1-induced arthritis animal model. We...
Macrophages belong to a special phagocytic subgroup of human leukocytes and are one the important cells immune system. Small noncoding RNAs group small RNA molecules that can be transcribed without ability encode proteins but could play specific function in cells. SncRNAs mainly include microRNAs (miRNAs) piwi-interacting (piRNAs), nucleolar (snoRNAs), nuclear (snRNAs) repeat RNAs. We used high-throughput sequencing analysis qPCR detect expression changes RNAome during macrophage...