A5068418625- Gene Regulatory Network Analysis
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Genetics, Bioinformatics, and Biomedical Research
- RNA Interference and Gene Delivery
- Bioinformatics and Genomic Networks
- Animal testing and alternatives
- Cell death mechanisms and regulation
- Cancer Genomics and Diagnostics
- Autophagy in Disease and Therapy
- Optimal Experimental Design Methods
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- 3D Printing in Biomedical Research
- Chemokine receptors and signaling
- Ovarian cancer diagnosis and treatment
- Cholesterol and Lipid Metabolism
Institute of Genetics and Biophysics
2010-2021
National Research Council
2015-2021
Charité - Universitätsmedizin Berlin
2008-2014
Max Planck Institute for Molecular Genetics
2012-2013
Max Planck Society
2012
Multiple reports implicated the function of G protein-coupled receptor (GPR)-30 with nongenomic effects estrogen, suggesting that GPR30 might be a G-protein coupled estrogen receptor. However, findings are controversial and expression pattern on cell type level as well its in vivo remains unclear. Therefore, objective this study was to identify types express Gpr30 by analyzing mutant mouse model harbors lacZ reporter (Gpr30-lacZ) locus leading partial deletion coding sequence. Using...
Increased expression of Bcl-xL in cancer has been shown to confer resistance a broad range apoptotic stimuli and modulate number other aspects cellular physiology, including energy metabolism, cell cycle, autophagy, mitochondrial fission/fusion adhesion. However, only few these activities have mechanistic explanation. Here we used Tandem Affinity purification identify novel interacting proteins that could explain the pleiotropic effects overexpression. Among several co-purifying with Bcl-xL,...
Granulosa cell tumor (GCT) is a rare and severe form of sex-cord stromal ovarian that characterized by its long natural history tendency to recur years after surgical ablation. Because there no efficient curative treatment beyond surgery, ~20% patients die the consequences their tumor. However, very little known molecular etiology this pathology. About 70% GCT present with elevated circulating estradiol (E2). hormone increase growth progression in number cancers, we investigated possible...
Transfection is the process by which nucleic acids are introduced into eukaryotic cells. This fundamental in basic research for studying gene function and modulation of expression as well many bioprocesses manufacturing clinical-grade recombinant biologics from efficiency a critical parameter to increase biologics' productivity; right protocol has be identified ensure high transfection therefore product yield. Design experiments (DoE) mathematical method that become key tool bioprocess...