A5029373066- Liver physiology and pathology
- Pancreatic function and diabetes
- Renal and related cancers
- Liver Disease Diagnosis and Treatment
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Phagocytosis and Immune Regulation
- Organ Transplantation Techniques and Outcomes
- Transplantation: Methods and Outcomes
- Tissue Engineering and Regenerative Medicine
- Wnt/β-catenin signaling in development and cancer
- Cardiac Ischemia and Reperfusion
- Medical Imaging and Pathology Studies
- Mesenchymal stem cell research
- Genomic variations and chromosomal abnormalities
- 3D Printing in Biomedical Research
- Single-cell and spatial transcriptomics
- Genetic and Kidney Cyst Diseases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Renal Transplantation Outcomes and Treatments
- Lung Cancer Treatments and Mutations
Stem Cell Institute
2021-2024
Wellcome/MRC Cambridge Stem Cell Institute
2020-2024
University of Cambridge
2020-2024
Stanford University
2024
NIHR Cambridge Biomedical Research Centre
2020-2022
National Institute for Health Research
2020
Organoids regenerate human bile ducts Bile carry from the liver and gall bladder to small intestine, where it aids digestion. Cholangiocytes are epithelial cells that line modify as its transported through biliary tree. Chronic diseases involving cholangiocytes account for a large fraction of failure need transplantation. Because donors in short supply, Sampaziotis et al. used organoid technology develop cell-based therapy using tissue (see Perspective by Kurial Willenbring). Cholangiocyte...
Background and Aims Organoids provide a powerful system to study epithelia in vitro . Recently, this approach was applied successfully the biliary tree, series of ductular tissues responsible for drainage bile pancreatic secretions. More precisely, organoids have been derived from ductal tissue located outside (extrahepatic ducts; EHBDs) or inside liver (intrahepatic IHBDs). These share many characteristics, including expression cholangiocyte markers such as keratin (KRT) 19. However,...
Variability among pluripotent stem cell (PSC) lines is a prevailing issue that hampers not only experimental reproducibility but also large-scale applications and personalized cell-based therapy. This variability could result from epigenetic genetic factors influence behavior. Naive culture conditions minimize fluctuation, potentially overcoming differences in PSC line differentiation potential. Here we derived PSCs distinct mouse strains under naive show backgrounds have divergent capacity,...
The signalling pathways that maintain primed human pluripotent stem cells (hPSCs) have been well characterised, revealing a critical role for TGFβ/Activin/Nodal signalling. In contrast, the requirements of naïve pluripotency not fully established. Here, we demonstrate TGFβ is required to hPSCs. downstream effector proteins – SMAD2/3 bind common sites in and hPSCs, including shared genes. additionally active regulatory regions near Inhibiting hPSCs causes downregulation SMAD2/3-target genes...
Production of large quantities hepatocytes remains a major challenge for number clinical applications in the biomedical field. Directed differentiation human pluripotent stem cells (hPSCs) into hepatocyte-like (HLCs) provides an advantageous solution and protocols have been developed this purpose. However, these methods usually follow different steps liver development vitro, which is time consuming requires complex culture conditions. In addition, HLCs lack full repertoire functionalities...
ABSTRACT Background and aims Non-alcoholic fatty liver disease (NAFLD) is a major health care challenge new therapies are urgently needed. However, the mechanisms underlying remain to be understood. Indeed, studying NAFLD remains challenging due lack of model systems recapitulating different aspects human pathology. Human induced pluripotent stem cells (hiPSCs) offer unique opportunity address this limitation since they can differentiated into large quantity cells. Here, we took advantage...
Non-alcoholic fatty liver disease (NAFLD) is a major health care challenge and new therapies are urgently needed. However, the mechanisms underlying remain to be understood. Indeed, studying NAFLD remains challenging due lack of model systems recapitulating different aspects human pathology. Human induced pluripotent stem cells (hiPSCs) offer unique opportunity address this limitation since they can differentiated into large quantity cells. Here, we took advantage hiPSCs develop...
Non-alcoholic fatty liver disease (NAFLD) is a major health care challenge and new therapies are urgently needed. However, the mechanisms underlying remain to be understood. Indeed, studying NAFLD remains challenging due lack of model systems recapitulating different aspects human pathology. Human induced pluripotent stem cells (hiPSCs) offer unique opportunity address this limitation since they can differentiated into large quantity cells. Here, we took advantage hiPSCs develop...
The liver has been studied extensively due to the broad number of diseases affecting its vital functions. However, therapeutic advances, especially in regenerative medicine, are currently hampered by lack knowledge concerning human hepatic cell development. Here, we addressed this limitation describing developmental trajectories different types comprising fetal at single-cell resolution. These transcriptomic analyses revealed that sequential cell-to-cell interactions direct functional...
Abstract Production of large quantities hepatocytes remains a major challenge for number clinical applications in the biomedical field. Directed differentiation human pluripotent stem cells (hPSC) into hepatocyte-like (HLCs) provides an advantageous solution and protocols have been developed this purpose. However, these methods usually follow different steps liver development vitro which is time consuming requires complex culture conditions. In addition, HLCs lack full repertoire...
Hypothesis: Intravenously injected nanoparticles (NPs) containing stromal cell-derived factor-1α (SDF-1α) (SDF-NPs) could sustainably target the ischemia reperfusion (I/R)-injured myocardium and induce robust angiogenesis by increasing systemic EPC levels, thereby attenuating myocardial I/R injury. Methods: Male Wistar rats were subjected to insult ligating left anterior descending artery for 30 min followed reperfusion. At reperfusion, randomized receive intravenous injections of 5 mL/kg...
Abstract The signalling pathways that maintain primed human pluripotent stem cells (hPSCs) have been well characterised, revealing a critical role for TGFβ/Activin/Nodal signalling. In contrast, the requirements of naïve pluripotency not fully established. Here, we demonstrate TGFβ is required to hPSCs. downstream effector proteins – SMAD2/3 bind common sites in and hPSCs, including shared genes. additionally active regulatory regions near Inhibiting hPSCs causes downregulation...