Milled wood lignin (MWL), cellulolytic enzyme (CEL), and enzymatic mild acidolysis (EMAL) were isolated from different species characterized by various techniques. The EMAL protocol offered gravimetric yields 2−5 times greater than those of the corresponding MWL CEL. purities EMALs 3.75−10.6% higher their CELs, depending upon which they isolated. Molecular weight analyses showed that isolates fractions are not accessed other procedures evaluated, while 31P NMR spectroscopy revealed is more...

The recently developed protocol for isolating enzymatic mild acidolysis lignins (EMAL) coupled with the novel combination of derivatization followed by reductive cleavage (DFRC) and quantitative 31P NMR spectroscopy were used to better understand lignin isolation process from wood. EMAL is shown offer access at samples that are more representative overall present in milled DFRC/31P provided a detailed picture on effects conditions structure. More specifically, we have vibratory ball milling...

Over a number of years work in our laboratory has been developing new chemistry for the use cellulose nanocrystals (CNCs) as scaffolds creation nanomaterials with novel, stimuli responsive characteristics. Our takes advantage rigid nature CNCs, unique nanopattern etched on their surface form regularly spaced primary OH groups, and fact that these materials have all reducing end groups located one end. In this communication, method grafting amine-terminated monomers onto surface-modified CNCs...

The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on cellulose supports were investigated. dynamics extent related processes monitored by surface plasmon resonance (SPR) quartz crystal microbalance with dissipation monitoring (QCM-D). Amine groups installed the substrate chitosan from aqueous solution, which allowed for hIgG to physisorb acid media produced a functionalized high density (10 mg/m(2)). neutral alkaline conditions was found yield lower adsorbed...

Three wood species, eucalyptus grandis (E. grandis), southern pine (S. pine), and Norway spruce thermomechanical pulp (N. TMP) were pretreated by dissolution in the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride ([AMIM]Cl). The was regenerated from high yield recycling of nearly quantitative. lignin contents efficiencies cellulase enzymatic hydrolyses examined offering an understanding into IL pretreatment efficiency. components that remained within recycled ILs qualitatively...

We introduce a new method to modify films of nanofibrillated cellulose (NFC) produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC were prepared from mechanically disintegrated hydrogel, and then their surfaces carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated EDS/NHS chemistry, its reactivity verified with bovine serum albumin antihuman IgG. The surface carboxylation, EDC/NHS activation protein...

A generic approach for heterogeneous surface modification of cellulosic materials in aqueous medium, applicable a wide range functionalizations, is presented. In the first step, carboxymethyl cellulose (CMC) modified with azide or alkyne functionality, was adsorbed on substrate, thus, providing reactive sites azide-alkyne cycloaddition click reactions. second functional units complementary were reacted surface, coated by click-modified CMC. Selected model functionalizations diverse...

Films and hydrogels consisting of cellulose nanofibrils (CNF) were modified by covalent EDC/NHS coupling luminescent, water-dispersible carbon dots (CDs). Quartz crystal microgravimetry with dissipation monitoring (QCM-D) surface plasmon resonance (SPR) used to investigate the attachment CDs on carboxymethylated CNF (CM-CNF). As first reported use CD in nanocellulose products, we provide proof-of-concept for synthesis transparent fluorescent nanopaper its tunable luminescence as confirmed...

We present an approach to construct biocompatible and photoluminescent hybrid materials comprised of carbon quantum dots (CQDs) TEMPO-oxidized cellulose nanocrystals (TO-CNCs). First, the amino-functionalized (NH2-CQDs) were synthesized using a simple microwave method, TO-CNCs prepared by hydrochloric acid (HCl) hydrolysis followed TEMPO-mediated oxidation. The conjugation NH2-CQDs was conducted via carbodiimide-assisted coupling chemistry. TO-CNC@CQD nanomaterials characterized X-ray...

Films comprising nanofibrillated cellulose (NFC) are suitable substrates for flexible devices in analytical, sensor, diagnostic, and display technologies. However, some major challenges such developments include their high moisture sensitivity the complexity of current methods available functionalization patterning. In this work, we present a facile process tailoring surface wettability functionality NFC films by fast versatile approach. First, were coated with layer reactive nanoporous...

We investigated the adsorption and chemical conjugation of avidin its deglycosylated form, neutravidin, on films regenerated nanofibrillar cellulose. The dynamics extent biomolecular attachment were monitored in situ by quartz crystal microbalance microgravimetry ex via surface analyses with atomic force microscopy X-ray photoelectron spectroscopy. installation carboxyl groups cellulose after modification carboxymethylated (CMC) or TEMPO-oxidation significantly increases physisorption...

Ultrathin bicomponent films of cellulose and lignin derivatives were deposited on silica supports by spin coating, after conversion into the respective polymer precursor, they used as a model system to investigate interfacial phenomena relevant lignocellulose biocatalysis. Film morphology, surface chemical composition, wettability determined atomic force microscopy, X-ray photoelectron spectroscopy, water contact angle, respectively. Phase separation produced structures that resembled cell...

Corn stover, Norway spruce, and Eucalyptus grandis were pulverized to different degrees. These samples subjected quantitative analyses, upon the basis of predissolution into imidazolium chloride-based ionic liquids [amim]Cl [bnmim]Cl followed by labeling hydroxyl groups as phosphite esters (31)P NMR analysis. Analysis pulverization degrees provided semiempirical data chart solubility spruce in these liquids. Further method refinment afforded an optimized analysis lignin phenolic...

Three wood species including Eucalyptus grandis (E. grandis), Southern pine (S. pine), and Norway spruce thermomechanical pulp (N. TMP) were dissolved in the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride ([Amim]Cl), then they pretreated with small amounts of hydrochloric acid, as a function time. The materials regenerated from IL solutions determined to contain significantly higher lignin than original wood. Detailed analyses recovered revealed presence typical degradation...

We present an efficient approach to develop cellulose nanocrystal (CNC) hybrids with magnetically responsive Fe3O4 nanoparticles that were synthesized using the (Fe3+/Fe2+) coprecipitation. After 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-catalyzed oxidation of CNC, carbodiimide (EDC/NHS) was used for coupling amine-containing iron oxide achieved by dopamine ligand exchange (NH2–Fe3O4 NPs). The as-prepared (Fe3O4@CNC) further complexed Cu(II) ions produce specific protein binding...

We attached anti-human serum albumin (anti-HSA) affibody ligands on bacterial cellulose (BC) by EDC–NHS-mediated covalent conjugation and physical adsorption demonstrate their application for tubular biofiltration of blood proteins.

Suberin is present in the underground parts of vegetables and bark trees. Characterization suberin structure its polyphenolic component have been hampered by insolubility polymers. Thus, enzymatically isolated extractive free enriched fraction from potato, Solanum tuberosum var. Nikola, chemically further fractionated phenolics were characterized solid state FTIR, DSC, elemental analysis to identify groups verify success isolation. For MW quantitative determination groups, polymers...

In this communication a method for the creation of fluorescent cellulose nanoparticles using click chemistry and subsequent photodimerization installed side-chains is demonstrated. first step, primary hydroxyl groups on surface CNCs were converted to carboxylic acids by TEMPO-mediated hypohalite oxidation. The alkyne groups, essential reaction, introduced into TEMPO-oxidized via carbodiimide-mediated formation an amide linkage between monomers carrying amine functionality acid CNCs. Finally,...