A5052175358- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Extracellular vesicles in disease
- Renal and related cancers
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Immunology Research
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Retinoids in leukemia and cellular processes
- T-cell and Retrovirus Studies
International Institute of Molecular and Cell Biology
2020-2025
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2020-2022
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2016-2017
Polish Academy of Sciences
2013
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2012
Among numerous enzymes involved in RNA decay, processive exoribonucleases are the most prominent group responsible for degradation of entire molecules. The role mammalian cytoplasmic 3'-5' exonuclease DIS3L at organismal level remained unknown. Herein, we established knock-in and knock-out mouse models to study functions mice. mice is indeed a subunit exosome complex, disruption which leads severe embryo degeneration death soon after implantation. These changes could not be prevented by...
It is becoming increasingly evident that the RNA degradome a crucial component of total cellular pool. Here, we present an analysis medium-sized RNAs (midi RNAs) form in Arabidopsis thaliana. Our analyses revealed midi fraction contained mostly 20-70-nt-long fragments derived from various species, including tRNA, rRNA, mRNA and snRNA. The majority these could be classified as stable degradation intermediates (RNA degradants). Using two dimensional polyacrylamide gel electrophoresis,...
One of the post-transcriptional mechanisms regulating stability RNA molecules involves addition non-templated nucleotides to their 3' ends, a process known as tailing. To systematically investigate physiological consequences terminal nucleotidyltransferase TENT2 absence on end modifications in mouse hippocampus, we developed new Tent2 knockout mouse. Electrophysiological measurements revealed increased excitability KO hippocampal neurons, and behavioral analyses showed decreased anxiety...
A pool of small RNA fragments (RFs) derived from diverse cellular RNAs has recently emerged as a rich source functionally relevant molecules. Although their formation and accumulation been connected to various stress conditions, the knowledge on RFs produced upon viral infections is very limited. Here, we applied next generation sequencing (NGS) characterize generated in hepatitis C virus (HCV) cell culture model (HCV-permissive Huh-7.5 line). We found that both infected non-infected cells...
One of the post-transcriptional mechanisms regulating stability RNA molecules involves addition non-templated nucleotides to their 3' ends, a process known as tailing. To systematically investigate physiological consequences terminal nucleotidyltransferase TENT2 absence on end modifications in mouse hippocampus we developed new Tent2 knockout mouse. Electrophysiological measurements revealed increased excitability KO hippocampal neurons, and behavioral analyses showed decreased anxiety...
Hepatitis C virus (HCV) infection is one of the major causes chronic liver diseases. Unfortunately, mechanisms HCV infection-induced injury and host-virus interactions are still not well recognized. To better understand these processes we determined changes in host gene expression that occur during Huh-7.5 cells. As a result, identified genes may contribute to immune metabolic cellular responses infection. Pathway enrichment analysis indicated induced an increased involved mitogen-activated...
Processive exoribonucleases are executors of RNA decay. In humans, their physical but not functional interactions were thoughtfully investigated. Here we have screened cells deficient in DIS3, XRN2, EXOSC10, DIS3L, and DIS3L2 with a custom siRNA library determined genetic (GIs) diverse pathways metabolism. We uncovered complex network positive that buffer alterations degradation reveal reciprocal cooperation genes involved transcription, export, splicing. Further, evaluated the distinctness...
Abstract Among numerous enzymes involved in RNA decay, processive exoribonucleases are the most prominent group responsible for degradation of entire molecules. The role mammalian cytoplasmic 3’-5’ exonuclease DIS3L at organismal level remained unknown. Herein we established knock-in and knock-out mouse models to study functions mice. is indeed a subunit exosome complex, which disruption leads severe embryo degeneration death mice soon after implantation. These changes could not be prevented...
ABSTRACT Processive exoribonucleases, the executors of RNA decay, participate in multiple physical and functional interactions. Unlike ones, relationships have not been investigated human cells. Here we screened cells deficient DIS3, XRN2, EXOSC10, DIS3L, DIS3L2 with a custom siRNA library determined their interactions diverse pathways metabolism. We uncover complex network positive that buffer alterations degradation. reveal important reciprocal actions between decay transcription explore...
Background.Diabetic patients present an increased risk for heart failure (HF) independently of the presence coronary artery disease (CAD) and hypertension.However, little is known about circulatory microRNA (miRNA), important regulatory RNA in this population. Objectives.To evaluate serum miRNA profile with diabetes mellitus (DM) HF analyze its relationship pathophysiological pathways involved. Materials methods.The accumulation 179 miRNAs was measured diabetic compared to same measurements...