A5024727634- Hepatitis C virus research
- RNA modifications and cancer
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Circular RNAs in diseases
- RNA regulation and disease
- Insect-Plant Interactions and Control
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Machine Learning in Bioinformatics
- Biosensors and Analytical Detection
- DNA and Biological Computing
- Planarian Biology and Electrostimulation
- Plant Reproductive Biology
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis Viruses Studies and Epidemiology
- Nitrogen and Sulfur Effects on Brassica
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2013-2025
Poznań University of Technology
2004-2016
Polish Academy of Sciences
2010-2013
CHA University
2009
Identification of biomarkers that could be used for the prediction response to neoadjuvant radiotherapy (neo-RT) in locally advanced rectal cancer remains a challenge addressed by different experimental approaches. Exosomes and other classes extracellular vesicles circulating patients' blood represent novel type liquid biopsy source biomarkers. Here, we combined proteomic metabolomic approach based on mass spectrometry techniques studying molecular components exosomes isolated from serum...
It is becoming increasingly evident that the RNA degradome a crucial component of total cellular pool. Here, we present an analysis medium-sized RNAs (midi RNAs) form in Arabidopsis thaliana. Our analyses revealed midi fraction contained mostly 20-70-nt-long fragments derived from various species, including tRNA, rRNA, mRNA and snRNA. The majority these could be classified as stable degradation intermediates (RNA degradants). Using two dimensional polyacrylamide gel electrophoresis,...
Abstract Phloem, an exceptional plant vascular tissue, facilitates the transport of photoassimilates, RNAs, and other signaling substances from leaves to roots throughout plant. Among specialized phloem cells are conductive sieve elements (SEs), which unique in that they remain alive despite lacking several cell organelles, including nucleus, plastids, most mitochondria. These SEs contain a specific proteinaceous structure composed phloem-specific proteins (P-proteins), whose function is not...
Abstract Background Despite advancements in genome annotation tools, challenges persist for non-classical model organisms with limited genomic resources, such as Schmidtea mediterranea . Findings To address these challenges, we developed a flexible and scalable pipeline that integrates short-read (Illumina) long-read (PacBio) sequencing technologies. The combines reference-based de novo assembly methods, effectively handling variability alternative splicing events. Functional is conducted to...
A pool of small RNA fragments (RFs) derived from diverse cellular RNAs has recently emerged as a rich source functionally relevant molecules. Although their formation and accumulation been connected to various stress conditions, the knowledge on RFs produced upon viral infections is very limited. Here, we applied next generation sequencing (NGS) characterize generated in hepatitis C virus (HCV) cell culture model (HCV-permissive Huh-7.5 line). We found that both infected non-infected cells...
In this study, biomimetic virus-like particles (VLPs) composed of superparamagnetic iron oxide nanoparticles (SPIONs) and receptor binding domain (RBD) the SARS-CoV-2 virus were obtained. The bioactivity VLPs was subsequently tested, utilizing natural targets RBD protein: angiotensin-converting enzyme 2 (ACE2), toll-like 4 (TLR4), neutralizing anti-SARS-CoV-2 antibodies. conducted analyses include both in vitro cellulo methods such as dynamic light scattering (DLS), ζ-potential measurement,...
Accumulating evidence suggests that certain features of hepatitis C virus (HCV), especially its high genetic variability, might be responsible for the low efficiency anti-HCV treatment. Here, we present a bioinformatic analysis HCV-1a populations isolated from 23 children with chronic (CHC) subjected to interferon-ribavirin therapy. The structures viral quasispecies were established based on 132-amino-acid sequence derived E1/E2 protein, including hypervariable region 1 (HVR1). Two types HCV...
RNA-seq is currently the only method that can provide a comprehensive landscape of circular RNA (circRNAs) in whole organism and its particular organs. Recent years have brought an increasing number RNA-seq-based reports on plant circRNAs. Notably, picture they revealed questionable depends applied circRNA identification quantification techniques. In consequence, little known about biogenesis functions circRNAs plants. this work we tested two experimental six bioinformatics procedures...
The continuously growing interest in small regulatory RNA exploration is one of the important factors that have inspired recent development new high throughput techniques such as DNA microarrays or next generation sequencing. Each these methods offers some significant advantages but at same time each them expensive, laborious and challenging especially terms data analysis. Therefore, there still a need to develop analytical enabling fast, simple cost-effective examination complex mixtures....
Pepino mosaic virus (PepMV) has the potential to cause serious tomato disease.Several isolates of PepMV were collected between 2003-2010
Hepatitis C virus (HCV) infection is one of the major causes chronic liver diseases. Unfortunately, mechanisms HCV infection-induced injury and host-virus interactions are still not well recognized. To better understand these processes we determined changes in host gene expression that occur during Huh-7.5 cells. As a result, identified genes may contribute to immune metabolic cellular responses infection. Pathway enrichment analysis indicated induced an increased involved mitogen-activated...