A5030054207- Gut microbiota and health
- Dermatology and Skin Diseases
- Chromosomal and Genetic Variations
- Genomic variations and chromosomal abnormalities
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Pluripotent Stem Cells Research
- Cancer Immunotherapy and Biomarkers
- Genetic Neurodegenerative Diseases
- Plant nutrient uptake and metabolism
- Redox biology and oxidative stress
- Sirtuins and Resveratrol in Medicine
- Endoplasmic Reticulum Stress and Disease
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Planarian Biology and Electrostimulation
- Neuroscience and Neuropharmacology Research
- Inflammatory Biomarkers in Disease Prognosis
- Neurogenesis and neuroplasticity mechanisms
- SARS-CoV-2 and COVID-19 Research
- ATP Synthase and ATPases Research
- Postharvest Quality and Shelf Life Management
- SARS-CoV-2 detection and testing
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2020-2025
Poznań University of Technology
2013-2017
Adam Mickiewicz University in Poznań
2015
Recent studies revealed a significant role of the gut fungal community in human health. Here, we investigated content and variation mycobiota among subjects from European population. We explored interplay between fungi various host-related sociodemographic, lifestyle, health, dietary factors. The study included 923 participants. Fecal DNA samples were analyzed by whole-metagenome high-throughput sequencing. Subsequently, taxonomic profiles determined accompanied computational statistical...
Abstract Recent research indicates that gut microbiota may be vital in the advancement of melanoma. In this study, we found melanoma patients exhibited a distinct mycobiota structure compared with healthy participants. Candida albicans, dubliniensis, and Neurospora crassa were more abundant samples from melanoma, whereas Saccharomyces cerevisiae Debaryomyces hansenii less abundant. During anti–PD-1 treatment, relative amount Malassezia restricta C. albicans increased. A higher level...
Abstract In recent years, the number of metagenomic studies increased significantly. Wide range factors, including tremendous community complexity and variability, is contributing to challenge in reliable microbiome profiling. Many approaches have been proposed overcome these problems making hardly possible compare results different studies. The significant differences between procedures used research are reflected a variation obtained results. This calls for need standardisation procedure,...
Leaf senescence is a tightly regulated developmental or stress-induced process. It accompanied by dramatic changes in cell metabolism and structure, eventually leading to the disintegration of chloroplasts, breakdown leaf proteins, internucleosomal fragmentation nuclear DNA ultimately death. In light global intense reorganization senescing transcriptome, measuring time-course gene expression patterns this model challenging due evident problems associated with selecting stable reference...
Copy number variations (CNVs) greatly contribute to intraspecies genetic polymorphism and phenotypic diversity. Recent analyses of sequencing data for >1000 Arabidopsis (Arabidopsis thaliana) accessions focused on small did not include CNVs. Here, we performed genome-wide analysis identified large indels (50 499 bp) CNVs (500 bp larger) in these accessions. The fully overlap with 18.3% protein-coding genes, enrichment evolutionarily young genes involved stress defense. By combining both...
Identification of biomarkers that could be used for the prediction response to neoadjuvant radiotherapy (neo-RT) in locally advanced rectal cancer remains a challenge addressed by different experimental approaches. Exosomes and other classes extracellular vesicles circulating patients' blood represent novel type liquid biopsy source biomarkers. Here, we combined proteomic metabolomic approach based on mass spectrometry techniques studying molecular components exosomes isolated from serum...
Huntington’s disease (HD) is a polyglutamine neurodegenerative involving pathogenesis within the striatum and cerebral cortex neurodevelopmental component, particularly in juvenile HD form (JOHD). We established fused dorsal-ventral system, imitating interaction single organoid to discover impairments at level of cell populations. found range early pathogenic phenotypes indicating that brain development affected by impaired neurogenesis. The occurred already early-stage 60-day organoids...
Huntington’s disease (HD) is a polyglutamine neurodegenerative involving pathogenesis within the striatum and cerebral cortex neurodevelopmental component, particularly in juvenile HD form (JOHD). We established fused dorsal-ventral system, imitating interaction single organoid to discover impairments at level of cell populations. found range early pathogenic phenotypes indicating that brain development affected by impaired neurogenesis. The occurred already early-stage 60-day organoids...
Abstract Research has shown that the microbiome can influence how immune system responds to melanoma cells, affecting course of disease and outcome therapy. Here, we used metagenomic approach flow cytometry analyses blood cells discover correlations between gut fungi metastatic patients enrolled in anti-PD-1 therapy lymphocytes their blood. We analyzed patterns associations before first administration (BT, n = 61) third month (T3, 37), allowing us track changes during treatment. To...
Abstract Phloem, an exceptional plant vascular tissue, facilitates the transport of photoassimilates, RNAs, and other signaling substances from leaves to roots throughout plant. Among specialized phloem cells are conductive sieve elements (SEs), which unique in that they remain alive despite lacking several cell organelles, including nucleus, plastids, most mitochondria. These SEs contain a specific proteinaceous structure composed phloem-specific proteins (P-proteins), whose function is not...
Abstract Background Despite advancements in genome annotation tools, challenges persist for non-classical model organisms with limited genomic resources, such as Schmidtea mediterranea . Findings To address these challenges, we developed a flexible and scalable pipeline that integrates short-read (Illumina) long-read (PacBio) sequencing technologies. The combines reference-based de novo assembly methods, effectively handling variability alternative splicing events. Functional is conducted to...
Transglutaminases (E.C. 2.3.2.13) catalyze the post-translational modification of proteins by establishing ε-(γ-glutamyl) lysine isopeptide bonds and covalent conjugation polyamines to endo-glutamyl residues proteins. In light confirmed role transglutaminases in animal cell apoptosis only limited information on these enzymes plant senescence, we decided investigate activity chloroplast (ChlTGases) fate chloroplast-associated Hordeum vulgare L. 'Nagrad' leaves, where senescence process was...
Intraspecies copy number variations (CNVs), defined as unbalanced structural of specific genomic loci, ≥1 kb in size, are present the genomes animals and plants. A growing examples indicate that CNVs may have functional significance contribute to phenotypic diversity. In model plant Arabidopsis thaliana at least several hundred protein-coding genes might display CNV; however, locus-specific genotyping studies this not been conducted. We analyzed natural region overlapping MSH2 gene encodes...
In this study, biomimetic virus-like particles (VLPs) composed of superparamagnetic iron oxide nanoparticles (SPIONs) and receptor binding domain (RBD) the SARS-CoV-2 virus were obtained. The bioactivity VLPs was subsequently tested, utilizing natural targets RBD protein: angiotensin-converting enzyme 2 (ACE2), toll-like 4 (TLR4), neutralizing anti-SARS-CoV-2 antibodies. conducted analyses include both in vitro cellulo methods such as dynamic light scattering (DLS), ζ-potential measurement,...
Copy number variants (CNVs) are intraspecies duplications/deletions of large DNA segments (> 1 kb). A growing reports highlight the functional and evolutionary impact CNV in plants, increasing need for appropriate tools that enable locus-specific genotyping on a population scale. Multiplex ligation-dependent probe amplification (MLPA) is considered gold standard humans. Consequently, numerous commercial MLPA assays CNV-related human diseases have been created. We routinely genotype complex...
Cell senescence occurs as a part of developmental or stress-induced process. It is tightly regulated and involves sequence metabolic structural alterations, eventually leading to cell death. Dark-induced leaf useful model for studying senescence-related events. To facilitate the integration physiological molecular studies utilizing this model, we generated microarray data set providing time course gene expression profiles in senescing barley leaves. Here, describe detailed procedures...
MicroRNAs (miRNAs) regulate gene expression by RNA interference mechanism. In plants, miRNA genes (MIRs) which are grouped into conserved families, i.e. they present among the different plant taxa, involved in regulation of many developmental and physiological processes. The roles nonconserved MIRs-which MIRs restricted to one family, genus, or even species-are less recognized; however, them participate responses biotic abiotic stresses. Both over- underproduction miRNAs may influence...
<p>Supplementary Figure 3. Relative abundance of Candida glabrata in normal (18.5</p>
<p>Supplementary Figure 7. Relative abundance of selected species during anti-PD-1 treatment.</p>
<p>Supplementary Figure Legends and Tables 2-9</p>
<p>Supplementary Figure 4. Phylogenetic trees of mycobiota in (A) healthy and (B) melanoma subjects.</p>