A5065740358- Glioma Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Gut microbiota and health
- MicroRNA in disease regulation
- Retinal Development and Disorders
- Ion channel regulation and function
- bioluminescence and chemiluminescence research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- Probiotics and Fermented Foods
- Meat and Animal Product Quality
- Genomics, phytochemicals, and oxidative stress
- Histone Deacetylase Inhibitors Research
- Mitochondrial Function and Pathology
- Drug Transport and Resistance Mechanisms
- Metabolism, Diabetes, and Cancer
- Microbial Metabolites in Food Biotechnology
- Lipid Membrane Structure and Behavior
- Ginseng Biological Effects and Applications
- Bee Products Chemical Analysis
- Diet and metabolism studies
- Cell death mechanisms and regulation
Baylor College of Medicine
2002-2024
Duke Medical Center
2016-2017
Duke University
2016
Duke University Hospital
2016
Chinese Academy of Sciences
1987-2010
Sericultural Research Institute
2008-2010
Shanghai Institute of Materia Medica
1987-1994
University of Oslo
1986-1992
Diffuse intrinsic pontine glioma (DIPG), or high-grade brainstem (BSG), is one of the major causes brain tumor-related deaths in children. Its prognosis has remained poor despite numerous efforts to improve survival. Panobinostat, a histone deacetylase inhibitor, targeted agent that recently shown pre-clinical efficacy and entered phase I clinical trial for treatment children with recurrent progressive DIPG.A collaborative study was conducted using both genetic BSG mouse model driven by...
Abstract Diffuse intrinsic pontine glioma (DIPG) is a highly aggressive pediatric brainstem tumor genetically distinguished from adult GBM by the high prevalence of K27M mutation in histone H3 variant H3.3 (H3F3A). This reprograms H3K27me3 epigenetic landscape DIPG inhibiting H3K27-specific methyltransferase EZH2. globally reduces H3K27me2/3, critical repressive marks responsible for cell fate decisions, and also causes focal gain throughout epigenome. To date, tumor-driving effects H3.3K27M...
Abstract Diffuse intrinsic pontine glioma (DIPG) is an incurable pediatric brain tumor, with approximately 25% of DIPGs harboring activating ACVR1 mutations that commonly co-associate H3.1K27M mutations. Here we show in vitro expression R206H and without upregulates mesenchymal markers activates Stat3 signaling. In vivo or G328V p53 deletion induces glioma-like lesions but not sufficient for full gliomagenesis. However, combination PDGFA signaling, significantly decrease survival increase...
Aims: The purpose of this study was to investigate the possible mechanism(s) saponins from Panax japonicus (SPJ) on alcohol-induced hepatic damage in mice. Methods: SPJ were identified by high performance liquid chromatography-evaporative light scattering detection-mass spectrometry (LC–ELSD–MS). Non-toxic concentrations assayed injury male ICR mice and human cells. protective effects evaluated biochemical values, histopathological observations relative gene expression. Results.In vitro,...
Dasatinib is a multikinase inhibitor in clinical trials for glioma, and thus far has failed to demonstrate significant efficacy. We investigated whether the ABC efflux transporters ABCG2 ABCB1 expressed blood-brain barrier (BBB), are limiting efficacy of dasatinib treatment glioma using genetic pharmacologic approaches. utilized brainstem mouse model driven by platelet-derived growth factor-B p53 loss abcg2/abcb1 wild-type (ABC WT) or knockout mice KO). First, we observed that tumor latency...
The purpose of this study was to investigate the protective effects sericin protein (SP) on alcohol-induced hepatic injury in mice and possible mechanisms.SP (0.375, 0.75 1.50 g/kg body weight) dissolved distilled water given by gavage 1 hour before alcohol (56% wt/vol, 14.2 ml/kg b.w.) treatment for 30 days, then blood, urine liver were collected, processed used concentration mensuration, various biochemical estimations histopathological examination.The evidently decreased serum increased...
Diffuse intrinsic pontine glioma (DIPG) is a rare and incurable brain tumor that arises predominately in children involves the pons, structure along with midbrain medulla makes up brainstem. We have previously developed genetically engineered mouse models of brainstem using RCAS/Tv-a system by targeting PDGF-B overexpression, p53 loss, H3.3K27M mutation to Nestin-expressing progenitor cells neonatal mouse. Here we describe novel model these same genetic alterations Pax3-expressing cells,...
Background Diffuse midline glioma (DMG) is an incurable brain cancer without a single FDA-approved drug that prolongs survival. CDK4/6 inhibitors have been evaluated in children with DMG limited efficacy. Since MAPK pathway activation upstream of cell proliferation, we hypothesized MEK may increase the anti-tumor effects inhibitors. Here, efficacy inhibitor ribociclib and trametinib human murine models to investigate combinational effects. Methods We conducted vitro vivo assays using lines...
Although the potent anti-parkinsonian action of atypical D₁-like receptor agonist SKF83959 has been attributed to selective activation phosphoinositol(PI)-linked D₁ receptor, whereas mechanism underlying its neuroprotective effect is not fully understood. In present study, actions on neuronal membrane potential and excitability were investigated in CA1 pyramidal neurons rat hippocampal slices. (10-100 µM) caused a concentration-dependent depolarization, associated with reduction input...
Metabotropic glutamate receptors (mGluRs) operate via the phosphoinositide second messenger cascade and have various modulatory effects on central neurones. 2-Amino-3-phosphonopropionate (AP3) has been proposed as a selective antagonist of mGluR used to explore physiological functions mGluR. We compared agonists in presence absence AP3 using intracellular recording from CA1 pyramidal neurones rat hippocampal slices. Two mM D,L-AP3 or 1 L-AP3 did not cause any detectable change...
A slow prepotential preceding the action potential elicited by 40–80 ms depolarizing current pulses injected in CA1 pyramidal cells rat hippocampal slices was isolated and characterized using a subtraction procedure. The exponentially rising showed enhanced amplitude at depolarized membrane levels. In sufficient doses, intracellular injection of lidocaine derivative QX‐314 selectively blocked prepotential, leaving largely unchanged. These results suggest that might be mediated persistent...
Microbiota-derived metabolites have emerged as key regulators of longevity. The metabolic activity the gut microbiota, influenced by dietary components and ingested chemical compounds, profoundly impacts host fitness. While benefits prebiotics are well-known, chemically targeting microbiota to enhance fitness remains largely unexplored. Here, we report a novel approach induce pro-longevity bacterial metabolite in gut. We discovered that specific
Diffuse intrinsic pontine glioma (DIPG) remains an incurable pediatric tumor with a median survival of less than one year and no approved chemotherapy. Recent studies using DIPG tissue revealed prevalent H3.3K27M mutations restructure the epigenome. The histone mutation causes globally reduced H3K27me3 repression localized peaks including at p16 promoter. suppressor is CDK4/6 inhibitor that regulates proliferation commonly deleted in many cancers but rarely DIPG. Therefore, inhibition...
Diffuse intrinsic pontine glioma (DIPG) is one of the major causes brain tumor-related death in children. Its prognosis has remained poor despite numerous efforts to improve survival. Panobinostat, a histone deacetylase inhibitor, targeted agent that recently entered phase I clinical trial for treatment children with recurrent or progressive DIPG. A co-clinical study using transgenic mouse model DIPG driven by PDGF-B signaling, p53 loss, and ectopic H3.3-K27M H3.3-WT expression investigate...
Diffuse intrinsic pontine glioma (DIPG) is a fatal brain cancer of childhood with median survival less than 1 year from diagnosis. The mitogen-activated protein kinase (MAPK) pathway (RAS-RAF-MEK1/2-ERK1/2) known signaling cascade in tumorigenesis. Trametinib an allosteric inhibitor MEK1/2. We performed pre-clinical study using genetically engineered mouse model DIPG driven by PDGF-B signaling, p53 loss, and ectopic H3.3-K27M expression to investigate the efficacy trametinib both vitro vivo....
Diffuse intrinsic pontine gliomas (DIPGs) comprise 15% of pediatric brain tumors and are the leading cause death for children with tumors. In spite numerous clinical trials, little progress has been made in prolonging survival DIPG patients. As there currently no effective treatments, it is important to develop mouse models order understand biology these Recently we, others have discovered activating bone morphogenic protein (BMP) pathway mutations ACVR1, which encodes Activin A receptor...
Diffuse intrinsic pontine glioma (DIPG) is an incurable pediatric brain tumor, resulting in the death of 200–300 children each year United States. Recently it was discovered that approximately 25% all DIPG cases harbor activating ACVR1mutations, a gene encodes Activin A receptor (ALK2), bone morphogenetic protein (BMP) pathway, and DIPGs with ALK2 mutations commonly H3.1K27M mutation. Herein, we used RCAS/TVA retroviral system to study effects ACVR1 on pathogenesis. In vitro expression R206H...