A5101521209- RNA Research and Splicing
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Liver Disease Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Pharmaceutical and Antibiotic Environmental Impacts
- Growth Hormone and Insulin-like Growth Factors
- Reproductive biology and impacts on aquatic species
- Microtubule and mitosis dynamics
- Insect and Pesticide Research
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Environmental Toxicology and Ecotoxicology
- Renal and related cancers
- Molecular Biology Techniques and Applications
- Ion channel regulation and function
- Plant Molecular Biology Research
- Diet, Metabolism, and Disease
- Hormonal and reproductive studies
- Inflammatory mediators and NSAID effects
- Neuroendocrine Tumor Research Advances
- Pharmacological Effects and Assays
- Radiopharmaceutical Chemistry and Applications
- Endoplasmic Reticulum Stress and Disease
University of California, San Francisco
2018-2023
Reproductive Science Center
2018-2023
Broad Center
2018-2020
University of California, Berkeley
2014-2016
Museum of Vertebrate Zoology
2016
Integra (United States)
2014
During oocyte maturation, changes in gene expression depend exclusively on translation and degradation of maternal mRNAs rather than transcription. Execution this program is essential for assembling the molecular machinery required meiotic progression, fertilization, embryo development. With present study, we used a RiboTag/RNA-Seq approach to explore timing mRNA quiescent oocytes as well progressing through first division. This genome-wide analysis reveals global switch coinciding with...
Abstract Deleted in azoospermia-like (DAZL) is an RNA-binding protein critical for gamete development. In full-grown oocytes, the DAZL increases 4-fold during reentry into meiotic cell cycle. Here, we have investigated functional significance of this accumulation at a genome-wide level. Depletion causes block maturation and widespread disruption pattern ribosome loading on maternal transcripts. addition to decreased translation, depletion also translational activation distinct subset mRNAs...
Cyclins associate with cyclin-dependent serine/threonine kinase 1 (CDK1) to generate the M phase-promoting factor (MPF) activity essential for progression through mitosis and meiosis. Although cyclin B1 (CCNB1) is required embryo development, previous studies concluded that CCNB2 dispensable cell cycle progression. Given findings of high
Abstract The molecular causes of deteriorating oocyte quality during aging are poorly defined. Since developmental competence relies on post-transcriptional regulations, we tested whether defective mRNA translation contributes to this decline in quality. Disruption ribosome loading maternal transcripts is present old oocytes. Using a candidate approach, detect altered 3’-UTR-reporters and poly(A) length the endogenous mRNAs. polyadenylation depends cytoplasmic binding protein 1 (CPEB1)....
Thyroid hormones are important modulators of metabolic activity in mammals and alter cholesterol fatty acid levels through activation the nuclear thyroid hormone receptor (THR). Currently, there several THRβ agonists clinical trials for treatment non-alcoholic steatohepatitis (NASH) that have demonstrated potential to reduce liver fat restore function. In this study, we tested three THRβ-agonism-based NASH candidates, GC-1 (sobetirome), MGL-3196 (resmetirom), VK2809, compared their...
Summary During oocyte maturation, changes in gene expression depend exclusively on translation and degradation of maternal mRNAs rather than transcription. Execution this program is essential for assembling the molecular machinery required meiotic progression, fertilization, embryo development. With present study, we used a RiboTag/RNA-Seq approach to explore timing mRNA quiescent oocytes as well progressing through first division. This genome-wide analysis reveals global switch coinciding...
ABSTRACT Cyclins associate with CDK1 to generate the M-phase-promoting factor (MPF) essential for progression through mitosis and meiosis. Previous studies concluded that CCNB2 is dispensable cell cycle progression. Given our findings of high translation rates CcnB2 mRNA in prophase-arrested oocytes, we have reevaluated its role during −/− oocytes undergo delayed germinal vesicle breakdown followed by a defective M-phase due reduced pre-MPF activity. This disrupted maturation associated...
Abstract Thyroid hormones are important modulators of metabolic activity in mammals and alter cholesterol fatty acid levels through activation the nuclear thyroid hormone receptor (THR). Currently, there several THRβ agonists clinical trials for treatment non-alcoholic steatohepatitis (NASH) that have demonstrated potential to reduce liver fat restore function. In this study, we tested three THRβ-agonism-based NASH candidates, GC-1 (sobetirome), MGL-3196 (resmetirom), VK2809, compared their...
Agonists of thyroid hormone receptor β (THR-β) decreased LDL cholesterol (LDL-C) and triglyceride (TG) levels in human clinical trials for patients with dyslipidemia. The authors present the highly potent selective compound ALG-055009 (
Abstract Deleted in azoospermia like (DAZL) is an RNA-binding protein playing critical function during gamete development. In fully-grown oocytes, DAZL detected prophase and levels increase four to five fold reentry into the meiotic cell cycle. Here, we have investigated functional significance of this accumulation maturing oocytes. Oocyte depletion prevents progression MII. This maturation block associated with widespread disruption pattern maternal transcripts loading on ribosomes their...