A5029858949- Sperm and Testicular Function
- Genital Health and Disease
- Neonatal Respiratory Health Research
- Urologic and reproductive health conditions
- Listeria monocytogenes in Food Safety
- Testicular diseases and treatments
- Urinary Bladder and Prostate Research
- Pneumonia and Respiratory Infections
- Microbial Inactivation Methods
- Bacteriophages and microbial interactions
- Sepsis Diagnosis and Treatment
- Congenital Diaphragmatic Hernia Studies
- Immune Response and Inflammation
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Sexual function and dysfunction studies
- Molecular Biology Techniques and Applications
- Orthopedic Infections and Treatments
- Neonatal and Maternal Infections
- Escherichia coli research studies
- Preterm Birth and Chorioamnionitis
- Bacterial Identification and Susceptibility Testing
- Poxvirus research and outbreaks
- Surgical site infection prevention
- Histone Deacetylase Inhibitors Research
Justus-Liebig-Universität Gießen
2010-2023
Klinikum St. Marien Amberg
2019-2022
Klinikum Weiden
2020-2022
Technische Hochschule Mittelhessen
2019
University of Maryland, Baltimore County
2018
Universitätsklinikum Gießen und Marburg
2004-2017
German Center for Infection Research
2016
Institute of Medical Microbiology and Hygiene
2001-2012
University of Tsukuba
2011
Zero to Three
2008
Listeria monocytogenes is a gram-positive, food-borne microorganism responsible for invasive infections with high overall mortality. L. among the very few microorganisms that can induce uptake into host cell and subsequently enter cytosol by breaching vacuolar membrane. We infected murine macrophage line P388D1 strain EGD-e examined gene expression profile of inside cytosolic environments using whole-genome microarray mutant analyses. found approximately 17% total genome was mobilized to...
The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow cytoplasm of vertebrate invertebrate cells following infection. general stress response, controlled by alternative sigma factor, σB, has an important role for bacterial survival both during We used quantitative real-time PCR analysis immuno-blot to examine σB expression growth L. EGD-e. Whole genome-based transcriptional profiling was identify...
Abstract Macrophages are important in the activation of innate immune responses and a tissue-specific manner maintenance organ homeostasis. Testicular macrophages (TM), which reside testicular interstitial space, comprise largest leukocyte population testes assumed to play relevant function maintaining privilege. Numerous studies have indicated that fluid (IF) surrounding TM has immunosuppressive properties, may influence phenotype TM. However, identity molecules present IF is poorly...
ABSTRACT Renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. In this study, a culture-independent 16S rRNA-based approach was established identify unusual, fastidious, or anaerobic investigate bacterial diversity specimens. Similarly sized amplicons encompassing the V6 V8 region of rRNA were analyzed with denaturing high-performance liquid...
The food-borne pathogen Listeria monocytogenes has the ability to survive extreme environmental conditions due an extensive interacting network of stress responses. It is able grow and at relatively high temperatures in comparison with other non-sporulating pathogens. To investigate heat-shock response L. monocytogenes, whole-genome expression profiles cells that were grown 37 degrees C exposed 48 examined using DNA microarrays. Transcription levels measured over a 40 min period after...
Infertility affects one in seven couples and ascending bacterial infections of the male genitourinary tract by Escherichia coli are an important cause factor infertility. Thus understanding mechanisms which immunocompetent cells such as testicular macrophages (TM) respond to infection how pathogens manipulate defense pathways is importance. Whole genome expression profiling TM peritoneal (PM) infected with uropathogenic E. (UPEC) revealed major differences regulated genes. However, a...
Abstract Background Multi-drug-resistant, extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, constitute an emerging public-health concern. Little data on the molecular epidemiology of ESBL producing Escherichia coli is available in Germany. Here we describe prevalence and producing- isolates at a German University hospital. Methods We analysed 63 non-duplicate clinical obtained over 8-month period using PCR sequence-based allele typing, plasmid replicon phylogenetic group...
Antibiotics-induced release of the pore-forming virulence factor pneumolysin (PLY) in patients with pneumococcal pneumonia results its presence days after lungs are sterile and is a major responsible for induction permeability edema. Here we sought to identify mechanisms mediating PLY-induced endothelial dysfunction. We evaluated hyperpermeability human lung microvascular cells (HL-MVECs) pulmonary artery vitro mice instilled intratracheally PLY. PLY increases monolayers by reducing stable...
Sepsis is the dysregulated host response to an infection which leads life-threatening organ dysfunction that varies by genomic factors. We conducted a genome-wide association study (GWAS) in 740 adult septic patients and focused on 28day mortality as outcome. Variants with suggestive evidence for (p≤10-5) were validated two additional GWA studies (n=3470) gene coding regions related variants assessed independent exome sequencing (n=74). In discovery GWAS, we identified 243 autosomal...
Summary Given the increasing prevalence of metabolic syndrome (MetS) in males reproductive age, objective this prospective case–controlled study was to investigate impact subacute systemic inflammation associated with MetS on seminal cytokines and standard sperm parameters comparison healthy men. Between 2011 2014, we recruited 27 patients out 41 obese screened from an internal outpatient clinic. Twenty‐seven age‐matched controls were enrolled 54 men requesting vasectomy a urological A...
CAP (Community acquired pneumonia) is frequent, with a high mortality rate and burden on health care systems. Development of predictive biomarkers, new therapeutic concepts, epidemiologic research require valid, reproducible, quantitative measure describing severity.Using time series data 1532 patients enrolled in the PROGRESS study, we compared putative measures severity for their utility as an operationalization. Comparison was based ability to correctly identify objectively severe state...
Objective: Patients encountering severe trauma are at risk of developing sepsis syndrome and subsequent multiple organ failure. This is often associated with fatal outcome despite survival the initial injury. We postulate that variation gene coding for tumor necrosis factor (TNF)-α increased occurrence mortality in patients. Design: Prospective cohort study; validation an external replication sample. Setting: Tertiary academic medical center. Patients: included 159 severely traumatized...
Summary A significant decline in human male reproductive function has been reported for the past 20 years but molecular mechanisms remain poorly understood. However, recent studies showed that gap junction protein connexin-43 (CX43; also known as GJA1) might be involved. CX43 is predominant testicular connexin (CX) most species, including humans. Alterations of its expression are associated with different forms spermatogenic disorders and infertility. Men impaired spermatogenesis often...
Infections caused by vancomycin-resistant Enterococcus faecium (VREfm) represent a major public health concern due to limited treatment options. Among invasive isolates of VREfm, ST117, ST80 and ST78 the most frequently detected STs MLST in Germany. In this study, we investigated genetic diversity VREfm recovered from different nosocomial outbreaks Bavaria, Germany, WGS.Between January 2018 April 2019, 99 non-replicate originating at eight hospitals Bavaria were for WGS. detail, complex...
Introduction Inflammation is a key driver of morbidity in the vulnerable preterm infant exposed to pre- and postnatal hazards significantly contributes chronic lung disease, i.e. bronchopulmonary dysplasia (BPD). However, early changes innate immunity associated with BPD development are incompletely understood. Methods In very immature infants below 32 weeks gestational age (GA; n=30 infants), monocyte subtypes were identified by Flow Cytometry at birth throughout course including...
During the last years tremendous changes have occurred in epidemiologic knowledge and diagnostic process of prostatitis syndrome. A new worldwide-accepted classification system has become gold standard contemporary literature. The aim this study was to compare inflammatory infectious status men with syndrome results from our cohort 1992. total 168 symptomatic (mean age 43.2 years; range 18-79) attending Giessen outpatient department were included. All underwent a four-glass-test including...
The clonal relationship between oral and fecal Candida albicans isolated from children of pre-school age was examined using RAPD analysis. Significantly higher levels C. were found in saliva, dental plaque, carious specimens stools 56 patients with severe caries as compared to 52 healthy control subjects. highest prevalence a strong correlation observed its presence specimen feces. analysis isolates 23 simultaneous revealed counterparts present both stool samples 15 cases; five harbored...
To test the hypothesis that lectin-like domain of tumor necrosis factor, mimicked by TIP peptide, can improve lung function after unilateral orthotopic isotransplantation. Because a lack specific treatment for ischemia reperfusion-mediated injury, accompanied disrupted barrier integrity and dysfunctional alveolar liquid clearance, alternative therapies restoring these parameters transplantation are required.Prospective, randomized laboratory investigation.University-affiliated...
In vivo application of histone deacetylase (HDAC) inhibitor trichostatin-A (TSA) in mice results male infertility. To get more insight into the mechanisms underlying this phenomenon, we performed a genome-wide expression analysis and investigated HDAC activity degree H3 H4 acetylation murine testes after TSA treatment. A significant decrease weak increase could be demonstrated at 2.5, 5.0, 7.5 hours application. Gene revealed 507 significantly regulated genes. Transcripts expressed somatic...
Cre-mediated excision of loxP sites is widely used in mice to manipulate gene function a tissue-specific manner. To analyze phenotypic alterations related Cre-expression, we have AMH-Cre-transgenic as model system. Different Cre expression levels were obtained by investigation C57BL/6J wild type well heterozygous and homozygous AMH-Cre-mice. Our results indicate that Cre-expression itself Sertoli cells already has led oxidative stress lipid peroxidation (4-HNE lysine adducts), inducing...