An approach using multivariate adaptive regression splines (MARSplines) was applied for quantitative structure-activity relationship studies of the antitumor activity anthrapyrazoles. At first stage, structures anthrapyrazole derivatives were subjected to geometrical optimization by AM1 method Polak-Ribiere algorithm. In next step, a data set 73 compounds coded over 2500 calculated molecular descriptors. It shown that fourteen independent variables appearing in statistically significant MARS...

A specific and reliable high-performance thin layer chromatography method with densitometry detection has been developed for the determination of naphazoline nitrate in nasal drops. The best separation basic analyte, without spot tailing, was achieved by using a mobile phase composed acetonitrile-water (60:40, v/v), adding 1.5 % (v/v) imidazolium-class ionic liquid covering plates stationary based on RP-18 F254S (10 × 20 cm). presented results confirm that imidazolium tetrafluoroborate...

The era of globalization causes that the export and import food from different continents world are becoming more common, which may directly contribute to increase in pollution them. presence mycotoxin is an ubiquitous problem. There very limited information on possible influence composition herbal mixtures mycotoxins them, area where research can be expanded. aim this study was determine patulin (PAT) commercial products containing dried elderberry, rose, blueberry, rowan, hawthorn,...

Abstract Novel oral anticoagulants are frequently used for the pharmacotherapy of thromboembolic disorders but still have drawbacks and side effects. While numerous synthetic semisynthetic derivatives nontoxic isosteviol possess potential therapeutic properties, including anticoagulant activity. Besides, thiourea is recognized in medicinal chemistry research as a component common framework many drugs or bioactive compounds. The present work combines molecular modeling docking approach...

Triazoloacridinones (TA) are a new group of potent antitumor compounds, from which the most active derivative, C-1305, has been selected for extended preclinical trials. This study investigated mechanism TA binding to DNA. Initially, six derivatives differing in chemical structures as well cytotoxicity and activity, capability noncovalent DNA was analyzed. We showed that all triazoloacridinones studied stabilized duplex at low-concentration buffer but not salt concentration corresponding...

Methods for determination of topiramate in pharmaceutical formulation by high-performance thin-layer chromatography (HPTLC) UV-densitometry and liquid chromatography-mass spectrometry (LC-MS) have been developed. HPTLC method as recommended United States Pharmacopeia was performed using the silica plates, mobile phase composed benzene:ethanol (5:2, v/v) densitometric detection at 340 nm after visualization with use chemical reagent. Moreover, quantification achieved concentration range...

Myasthenia gravis (MG) is an autoimmune disease characterized by weakness and rapid fatigue. Diagnostic methods used for myasthenia are not conclusive satisfactory, therefore it necessary to develop reliable tools help diagnose as early possible. The aim of the study was use HPLC-MS in conjunction with multivariate statistical analyses investigate changes amino acid metabolic profiles between patients compared controls. In addition, effect treatment regimens type, on observed were assessed....

Brain tumors account for 1% of all cancers diagnosed de novo. Due to the specificity anatomical area in which they grow, can cause significant neurological disorders and lead poor functional status disability. Regardless results biochemical markers intracranial neoplasms, are currently no diagnostic significance. The aim study was use LC-ESI-MS/MS conjunction with multivariate statistical analyses examine changes amino acid metabolic profiles between patients glioblastoma, meningioma, a...

The acridinone derivatives with antitumor activity and ability respect to noncovalent DNA binding were investigated for their quantitative structure-activity relationships (QSAR). Multiple regression analysis was used model between molecular descriptors antileukemia activity, or DNA-duplexes stabilization. Studies performed on modeling using HyperChem Dragon computer programs, geometry optimization MM+ mechanics semi-empirical AM1 method. Two multiple equations derived characterized as good...

Abstract The lipophilicity values of selected acridinone (imidazoacridinone and triazoloacridinone) derivatives were measured by gradient reversed-phase high-performance liquid chromatography (RP-HPLC) using a C18 stationary phase with water/acetonitrile mixture as mobile phase. retention times obtained served input data appropriate log kw (i.e., the factor extrapolated to 0% organic modifier) an alternative P calculated DryLab program. relationships between (log kw) chemical structure...

Dementia is a clinical syndrome characterized by cognitive impairment, in which there disturbance of multiple higher cortical functions. The primary risk factor dementia old age, and due to significant changes the worldwide demographic structure, prevalence impairment increasing dramatically with aging populations most countries. Alzheimer’s disease predominant leading cause dementia. aim this study was evaluate modifications amino acids that characterize initial stages help our...

Background: Multiple sclerosis (MS) and myasthenia gravis (MG) are autoimmune diseases that attack the central nervous system (CNS) neuromuscular junction, respectively. As common pathogenesis of both is associated with an background involvement T B lymphocytes, overlapping selected clinical symptoms may cause difficulties in differential diagnosis diseases. Methods: The aim study was to use Liquid Chromatography–Electrospray Ionization–Mass Spectrometry (LC–ESI–MS/MS) conjunction...