A5101748837- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Pulmonary Hypertension Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Renal and related cancers
- Epigenetics and DNA Methylation
- Lymphatic System and Diseases
- Cancer, Hypoxia, and Metabolism
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- Fungal and yeast genetics research
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Economic and Environmental Valuation
- Yeasts and Rust Fungi Studies
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Frequency Control in Power Systems
- Cardiovascular Function and Risk Factors
Louisiana State University Health Sciences Center Shreveport
2020-2025
National Institute of Food and Drug Safety Evaluation
2025
Emory University
2011-2024
Yonsei University
2011-2024
Louisiana State University Health Sciences Center New Orleans
2023
Children's Heart Center Nevada
2019
University of Illinois Chicago
2011-2016
Yale Cancer Center
2016
Korea Food Research Institute
2015-2016
Korea Advanced Institute of Science and Technology
2015
Bone marrow (BM)-derived mesenchymal stem cells (MSCs) hold great promise for cardiovascular cell therapy owing to their multipotency and culture expandability.The aim of the study was investigate whether MSCs can treat experimental acute myocardial infarction (MI) diabetic neuropathy.We isolated mononuclear from mouse BM cultured in a conventional manner. Flow cytometry analyses these at passage 4 showed expression typical MSC markers such as CD44 CD29, but not hematopoietic c-kit, flk1,...
The receptor tyrosine kinase FLK1 and the transcription factor SCL play crucial roles in establishment of hematopoietic endothelial cell lineages mice. We have previously used an vitro differentiation model embryonic stem (ES) cells demonstrated that develop via sequentially generated FLK1+ SCL+cells. To gain a better understanding cellular molecular events leading to specification, we examined factors necessary for SCL+ induction serum-free conditions. demonstrate bone morphogenetic protein...
Myocardial infarction and ischemic heart disease are the leading cause of death in industrial world. Therapies employed for treating these diseases aimed at promoting increased blood flow to cardiac tissue. Pharmacological induction new coronary growth has recently been explored, however, clinical trials with known proangiogenic factors have disappointing. To identify novel therapeutic targets, we explored signaling pathways that govern embryonic development. Using a combination genetically...
The mammalian ortholog of the Drosophila MOF (males absent on first) gene product is a histone H4 lysine 16-specific acetyltransferase. Recent studies have shown that depletion human (hMOF) in cell lines leads to genomic instability, spontaneous chromosomal aberrations, cycle defects, altered nuclear morphology, reduced transcription certain genes, and defective DNA damage response ionizing radiation (IR). Here we show plays an essential role mammals during embryogenesis oncogenesis....
Much knowledge of adipocyte biology has been learned from cell culture models, most notably 3T3-L1 cells. The model several limitations, including the requirement 2 weeks to generate adipocytes and waning adipogenic potential in culture. We have investigated capacity OP9 cells, a line bone marrow-derived mouse stromal recapitulate adipogenesis. When cells are given any one three stimuli, they rapidly accumulate triacylglycerol, assume morphology, express late marker proteins, glucose...
Significance FGF receptor (FGFR) signaling is thought to be essential for vascular development, homeostasis, and pathological angiogenesis. However, the in vivo requirements cellular targets of vasculature are not known. Here, we show that endothelial FGFR1 FGFR2 required homeostasis or physiological functions likely embryonic development. neovascularization after skin eye injury following retinal ischemia. These findings reveal a key requirement cell-autonomous FGFR tissue repair validate...
Direct conversion or reprogramming of human postnatal cells into endothelial (ECs), bypassing stem progenitor cell status, is crucial for regenerative medicine, therapy, and pathophysiological investigation but has remained largely unexplored.We sought to directly reprogram dermal fibroblasts ECs with vasculogenic transcription factors determine their vascularizing therapeutic potential.We utilized various combinations 7 EC transduce found that ER71/ETV2 (ETS variant 2) alone best induced...
Background— Emerging evidence has suggested a contribution of bone marrow (BM) cells to lymphatic vessel formation; however, the exact phenotype with endothelial progenitor cell function yet be identified. Here, we investigate identity BM-derived and their role in neovascularization. Methods Results— Culture BM-mononuclear presence vascular growth factors A C factor resulted expression markers. Among these cells, podoplanin + were isolated by magnetic-activated sorting characterized...
Abstract Human pluripotent stem cells (hPSCs) have emerged as an important source for cell therapy. However, to date, no studies demonstrated generation of purified hPSC-derived lymphatic endothelial (LECs) and tested their therapeutic potential in disease models. Here we sought differentiate hPSCs into the LEC lineage, purify them with markers evaluate effects. We found that OP9-assisted culture system reinforced by addition VEGF-A, VEGF-C EGF most efficiently generated LECs, which were...
Myct1 inhibition controls tumor angiogenesis, remodels immunity, and improves immunotherapy outcomes in mouse models.
Autism spectrum disorder (ASD) is a neurodevelopmental disability condition arising from combination of genetic and environmental factors. Despite the blood-brain barrier (BBB) serving as crucial gatekeeper, conveying influences into brain parenchyma, contributions BBB in ASD pathogenesis remain largely uncharted. Here we report that SHANK3, an ASD-risk gene, expresses BBB-forming endothelial cells (BECs) regulates tight junctional (TJ) integrity essential for BBB's function....
Bone morphogenetic protein 4 (BMP4) is crucial for the formation of FLK1-expressing (FLK1(+)) mesodermal cells. To further define requirement BMP signaling in differentiation blood, endothelial and smooth muscle cells from FLK1(+) mesoderm, we inactivated Alk3 (Bmpr1a) by crossing Alk3(floxed/floxed) Flk1(+/Cre)Alk3(+/floxed) mice. conditional knockout (CKO) mice died between E10.5 E11.5. Unexpectedly, CKO embryos did not show any hematopoietic defects. However, displayed multiple...
Objective— Comprehensive understanding of the mechanisms regulating angiogenesis might provide new strategies for angiogenic therapies treating diverse physiological and pathological ischemic conditions. The E-twenty six (ETS) factor Ets variant 2 (ETV2; aka Ets-related protein 71) is essential formation hematopoietic vascular systems. Despite its indispensable function in vessel development, ETV2 role adult has not yet been addressed. We have therefore investigated regeneration. Approach...
In this study, we identify and characterize a novel transmembrane adaptor protein, designated Lck-interacting membrane protein (LIME), as binding partner of the Lck Src homology (SH)2 domain. LIME possesses short extracellular domain, cytoplasmic tail containing five tyrosine-based motifs. The is primarily expressed in hematopoietic cells lung. Interestingly, expression up-regulated by TCR stimulation sustained up to 24 h, suggesting that acts throughout early late stages T cell activation....
Histone acetylation has been implicated in learning and memory the brain, however, its function at level of genome individual genetic loci remains poorly investigated. This study examines a key mark, histone H4 lysine 5 (H4K5ac), genome-wide role activity-dependent gene transcription adult mouse hippocampus following contextual fear conditioning. Using ChIP-Seq, we identified 23,235 genes which H4K5ac correlates with absolute expression hippocampus. However, absence factor binding sites 150...