A5082671116- S100 Proteins and Annexins
- Chemokine receptors and signaling
- Immune cells in cancer
- Axon Guidance and Neuronal Signaling
- Cell Adhesion Molecules Research
- Cannabis and Cannabinoid Research
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Cytokine Signaling Pathways and Interactions
- HIV Research and Treatment
- Protease and Inhibitor Mechanisms
- Epigenetics and DNA Methylation
- Advanced Glycation End Products research
- Inflammation biomarkers and pathways
- Cancer Mechanisms and Therapy
- Immune Cell Function and Interaction
- Estrogen and related hormone effects
- T-cell and B-cell Immunology
- Cancer Risks and Factors
- Immune Response and Inflammation
- Hepatitis C virus research
- Inflammatory mediators and NSAID effects
- Biomarkers in Disease Mechanisms
- Cancer, Hypoxia, and Metabolism
The Ohio State University
2015-2025
The Ohio State University Wexner Medical Center
2015-2024
Ohio University
2010-2024
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2024
Comprehensive Blood & Cancer Center
2021
Cancer Genetics (United States)
2009-2011
Ludwig-Maximilians-Universität München
2011
National Cancer Institute
2011
University of Zurich
2011
GTx (United States)
2011
The α-chemokine stromal cell-derived factor (SDF)-1α binds to the seven transmembrane G-protein-coupled CXCR-4 receptor and acts modulate cell migration proliferation. signaling pathways that mediate effects of SDF-1α are not well characterized. We studied events following binding in a model murine pre-B line transfected with human CXCR-4. There was enhanced tyrosine phosphorylation association components focal adhesion complexes such as related kinase, paxillin, Crk. also observed...
Abstract Cannabinoids have been reported to possess antitumorogenic activity. Not much is known, however, about the effects and mechanism of action synthetic nonpsychotic cannabinoids on breast cancer growth metastasis. We shown that cannabinoid receptors CB1 CB2 are overexpressed in primary human tumors compared with normal tissue. also observed cell lines MDA-MB231, MDA-MB231-luc, MDA-MB468 express receptors. Furthermore, we agonist JWH-133 WIN-55,212-2 inhibit proliferation migration...
The mammalian ortholog of the Drosophila MOF (males absent on first) gene product is a histone H4 lysine 16-specific acetyltransferase. Recent studies have shown that depletion human (hMOF) in cell lines leads to genomic instability, spontaneous chromosomal aberrations, cycle defects, altered nuclear morphology, reduced transcription certain genes, and defective DNA damage response ionizing radiation (IR). Here we show plays an essential role mammals during embryogenesis oncogenesis....
Abstract Non–small cell lung cancer (NSCLC) is the leading cause of deaths worldwide; however, only limited therapeutic treatments are available. Hence, we investigated role cannabinoid receptors, CB1 and CB2, as novel targets against NSCLC. We observed expression (24%) CB2 (55%) in NSCLC patients. Furthermore, have shown that treatment lines (A549 SW-1573) with CB1/CB2- CB2-specific agonists Win55,212-2 JWH-015, respectively, significantly attenuated random well growth factor-directed vitro...
Abstract RAGE is a multifunctional receptor implicated in diverse processes including inflammation and cancer. In this study, we report that expression upregulated widely aggressive triple-negative breast cancer (TNBC) cells, both primary tumors lymph node metastases. evaluating the functional contributions of cancer, found RAGE-deficient mice displayed reduced propensity for tumor growth. an established model lung metastasis, systemic blockade by injection neutralizing antibody inhibited...
S100A7/psoriasin, a member of the epidermal differentiation complex, is widely overexpressed in invasive estrogen receptor (ER)α-negative breast cancers. However, it has not been established whether S100A7 contributes to cancer growth or metastasis. Here, we report consequences its expression on inflammatory pathways that impact growth. Overexpression human murine homologue mS100a7a15 enhanced cell proliferation and upregulated various proinflammatory molecules ERα-negative cells. To examine...
c-Myc is a well-known oncogene frequently up-regulated in different malignancies, whereas liver-specific microRNA (miR)-122, bona fide tumor suppressor, down-regulated hepatocellular cancer (HCC). Here we explored the underlying mechanism of reciprocal regulation these two genes. Real-time reverse-transcription polymerase chain reaction (RT-PCR) and northern blot analysis demonstrated reduced expression primary, precursor, mature miR-122 c-MYC -induced HCCs compared to benign livers,...
Abstract Introduction Although C-X-C motif chemokine 12 (CXCL12) has been shown to bind receptor type 7 (CXCR7), the exact molecular mechanism regulations by CXCL12/CXCR7 axis in breast tumor growth and metastasis are not well understood. CXCR7 expression be upregulated during pathological processes such as inflammation cancer. Methods Breast cancer cell lines were genetically silenced or pharmacologically inhibited for and/or its downstream target signal transducer activator of...
Abstract Tumor-associated macrophages (TAM) are heterogeneous in nature and comprise antitumor M1-like (M1-TAM) or pro-tumor M2-like (M2-TAM) TAMs. M2-TAMs a major component of stroma breast tumors enhance metastasis by reducing their phagocytic ability increasing tumor fibrosis. However, the molecular mechanisms that regulate phenotypic plasticity TAMs not well known. Here we report novel suppressor Slit2 cancer regulating microenvironment. reduced vivo growth spontaneous syngeneic mammary...
Slit, which mediates its function by binding to the Roundabout (Robo) receptor, has been shown regulate neuronal and CXCR4-mediated leukocyte migration. Slit-2 was be frequently inactivated in lung breast cancers because of hypermethylation promoter region. Furthermore, CXCR4/CXCL12 axis reported recently actively involved cancer metastasis target organs such as lymph nodes, lung, bone. In this study, we sought characterize effect Slit (=Slit-2) on CXCL12/CXCR4-mediated metastatic properties...
Abstract Slit, which mediates its function by binding to the Roundabout (Robo) receptor, has been shown regulate neuronal, dendritic, and leukocyte migration. However, molecular mechanism Slit/Robo complex inhibits migration of cells is not well defined. Here, we showedthat Slit-2 can inhibit CXCL12-induced chemotaxis transendothelial T monocytes. We observed that CXCR4 associates with Robo-1 treatment enhances this association receptor. a single-pass transmembrane receptor whose...
Abstract Cell therapy with bone marrow multipotential stromal cells (MSCs) represents a promising approach to promote wound healing and tissue regeneration. MSCs expanded in vitro lose early progenitors differentiation therapeutic potentials under normoxic condition, whereas hypoxic condition promotes MSC self‐renewal through preserving colony forming maintaining undifferentiated phenotypes. Hypoxia inducible factor (HIF) pathway is crucial signaling activated condition. We evaluated the...
Cannabinoids bind to cannabinoid receptors CB(1) and CB(2) have been reported possess anti-tumorigenic activity in various cancers. However, the mechanisms through which cannabinoids modulate tumor growth are not well known. In this study, we report that a synthetic non-psychoactive specifically binds receptor may breast metastasis by inhibiting signaling of chemokine CXCR4 its ligand CXCL12. This pathway has shown play an important role regulating cancer progression metastasis.We observed...
Abstract The secretory protein Slit2 and its receptors Robo1 Robo4 are considered to regulate mobility permeability of endothelial cells other cell types. However, the roles two in inflammatory responses remain be clarified. In this study, we show that, primary HUVECs, represses LPS-induced secretion certain cytokines/chemokines, adhesion molecule ICAM-1 upregulation, monocyte adhesion. Slit2’s anti-inflammatory effect is mediated by dominant endothelial-specific receptor Robo4. minor has...
Tumor associated macrophages play a vital role in determining the outcome of breast cancer. We investigated contribution chemokine receptor CXCR3 to antitumor immune responses using cxcr3 deficient mouse orthotopically injected with PyMT cancer cell line. observed that mice displayed increased IL-4 production and M2 polarization tumors spleens compared WT cells. This was accompanied by larger tumor development cxcr3(-/-) than mice. Further, tumor-promoting myeloid derived populations...
JWH‐015, a cannabinoid receptor 2 (CB2) agonist has tumor regressive property in various cancer types. However, the underlying mechanism by which it acts lung is still unknown. Tumor associated macrophage (TAM) intensity positive correlation with progression. Also, macrophages recruited at site promote growth enhancing epithelial to mesenchymal (EMT) In this study, we analyzed role of JWH‐015 on EMT and infiltration regulation EGFR signaling. inhibited NSCLC cells A549 also reversed nature...