A5080981755- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Cystic Fibrosis Research Advances
- Infections and bacterial resistance
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Healthcare Regulation
- Antibiotic Use and Resistance
- Tracheal and airway disorders
- Tuberculosis Research and Epidemiology
- Microbial Applications in Construction Materials
- Infection Control in Healthcare
- Genomics and Phylogenetic Studies
- Bacillus and Francisella bacterial research
- Public Health in Brazil
- Actinomycetales infections and treatment
- Banana Cultivation and Research
- Environmental and biological studies
- Trauma Management and Diagnosis
- Streptococcal Infections and Treatments
- Nursing Roles and Practices
- Central Venous Catheters and Hemodialysis
- Cerebrospinal fluid and hydrocephalus
Universidade do Estado do Rio de Janeiro
2015-2025
Hospital Universitário Pedro Ernesto
2017-2025
We investigated the possibility of cross-infection among cystic fibrosis patients in two Brazilian reference centers. Achromobacter xylosoxidans isolates (n = 122) were recovered over a 5-year period from 39 patients. Isolates genetically heterogeneous, but one genotype was present 56% patients, suggesting that may have occurred.
SUMMARY Achromobacter spp. are opportunistic pathogens increasingly recovered from adult patients with cystic fibrosis (CF). We report the characterization of 122 isolates 39 CF by multilocus sequence typing, virulence traits, and susceptibility to antimicrobials. Two species, A. xylosoxidans (77%) ruhlandii (23%) were identified. All showed a similar biofilm formation ability, positive swimming phenotype. By contrast, 4·3% 44·4% , respectively, exhibited negative swarming phenotype, making...
To address the question whether ExoU, a Pseudomonas aeruginosa cytotoxin with phospholipase A2 activity, can induce hemostatic abnormalities during course of pneumosepsis, mice were instilled i.t. ExoU-producing PA103 P. or mutant obtained by deletion exoU gene. Control animals sterile vehicle. assess role ExoU in animal survival, evaluated for 72 h. In all other experiments, studied at 24 h after infection. PA103-infected showed significantly higher mortality rate, lower blood leukocyte...
Molecular methodologies were used to identify 28 Achromobacter spp. from patients with cystic fibrosis (CF). Multilocus sequence typing (MLST) identified 17 xylosoxidans isolates (all bla(OXA-114) positive), nine ruhlandii one dolens isolate, and insuavis isolate. All less common species misidentified as A. by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Chronic colonization clonally related was demonstrated.
Knowledge about the virulence mechanisms of species from Burkholderia cepacia complex (BCC) is still limited. The genomovar heterogeneity and production different factors are likely to contribute variation in clinical outcome observed BCC-infected cystic fibrosis (CF) patients. Therefore, this study we investigated genetic polimorphism, presence makers associated with transmissibility BCC, profile exoenzyme 59 BCC isolates obtained CF patients attending reference centre Rio de Janeiro,...
The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization antimicrobial resistance profile for isolates from blood. Most of all (81.9%) are related to known HA-MRSA CA-MRSA epidemic lineages, such as, USA300, USA400, USA600, USA800 USA1100. This is first multicenter study in Rio de Janeiro.
Multidrug-resistant microorganisms are a well-known global problem, and gram-negative bacilli top-ranking. When these pathogens associated with bloodstream infections (BSI), outcomes become even worse. Here we applied whole-genome sequencing to access information about clonal distribution, resistance mechanism diversity other molecular aspects of (GNB) isolated from in Brazil. It was possible highlight international high-risk clones circulating the Brazilian territory, such as CC258 for...
We describe the recovery of Paenibacillus cineris from a Brazilian cystic fibrosis (CF) patient. spp. are mainly environmental organisms. Although role that these species play in lung disease is not known, we highlight importance careful attention to unusual bacteria respiratory secretions CF patients.
Alarmingly, the isolation of methicillin-resistant Staphylococcus aureus (MRSA) has been increasing among patients with cystic fibrosis (CF). During a previous molecular characterisation MRSA isolates obtained from CF Rio de Janeiro, Brazil, one isolate was identified as ST398 clone, livestock-associated (LA) MRSA. In this study, we report draft genome sequence an LA-MRSA clone isolated patient CF.
Hypermutable (HPM) strains of Pseudomonas aeruginosa have been found at high frequencies in cystic fibrosis (CF) patients Europe. We report the results testing for HPM frequencies, mutator genotype, and antimicrobial resistance P. from Brazilian CF patients. A modified disk diffusion technique was used to quantify antibiotic-resistant subpopulations an isolate, estimations frequency mutation rifampin were determined 705 isolates 149 attending clinics two cities. Mutations mutS gene detected...
Abstract Acinetobacter spp. are important healthcare pathogens, being closely linked to antibiotic resistance and outbreaks worldwide. Although such species rarely observed in patients with cystic fibrosis (CF), we describe the characteristics of 53 strains isolated from sputum 39 Brazilian CF. The distribution was A. baumannii ( n = 29), pittii 13), nosocomialis 8), seifertii 1), soli 1) variabilis determined by partial rpoB gene sequencing. Sixteen (10 , 3 ) were multidrug-resistant (MDR)...
Ferreira AG, Leão RS, Carvalho‐Assef APD, Folescu TW, Barth AL, Marques EA. Influence of biofilm formation in the susceptibility Pseudomonas aeruginosa from Brazilian patients with cystic fibrosis. APMIS 2010; 118: 606–12. Biofilms play a key role occurrence lung infections by fibrosis (CF). In this study, we examined 40 isolates P. CF according to their capacity form biofilm. We also compared vitro response antimicrobials different modes growth (planktonic vs biofilm) and performed...
The clinical relevance of Achromobacter xylosoxidans infection in cystic fibrosis (CF) remains controversial. This emerging agent CF has been associated with increased lung inflammation, more frequent exacerbations and severe disease. We describe a pair siblings chronically colonized by the same multilocus genotype A. different courses, assess whether this species may have developed any virulence traits antimicrobial resistance that could contributed to their singular outcomes.Two were...
A prospective study was conducted in Brazil to evaluate antimicrobial resistance patterns and molecular epidemiology of Pseudomonas aeruginosa isolates from cystic fibrosis ( CF ) patients with chronic lung infection. All were obtained between May 2009 June 2010 75 seen four reference centers Brazil: HCPA (20 patients) HEOM (15 patients), located southern northeastern Brazil, respectively; IFF HUPE both southwestern Brazil. Antimicrobial susceptibility testing, PCR for detection...
contributes significantly to the global issue of multidrug-resistant (MDR) nosocomial infections. Often, these strains demonstrate resistance carbapenems (MDR-CRAB), first-line treatment for infections instigated by MDR
The prevalence of a variety potentially pathogenic microorganisms in cystic fibrosis patients, such as methicillin-resistant Staphylococcus aureus (MRSA), has increased over the past decade. Given increasing MRSA and few data available literature, better understanding clinical repercussions colonization by this bacterium patients becomes essential. This study aimed to evaluate chronic patients.Retrospective cohort from January 2004 December 2013 reference center.Each patient with was...
Persistent methicillin-resistant Staphylococcus aureus (MRSA) infection in cystic fibrosis (CF) patients has been associated with a more rapid decline lung function, increased hospitalisation and mortality. The aim of this study was to evaluate the clonal relationships among 116 MRSA isolates from 12 chronically colonised CF pediatric over 6-year period Rio de Janeiro specialist centre. Isolates were characterised by antimicrobial resistance, SCCmec type, presence Panton-Valentine Leukocidin...