A5046277831- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Enzyme Production and Characterization
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- Crystallization and Solubility Studies
- Pancreatic function and diabetes
- Nanoplatforms for cancer theranostics
- Antibiotics Pharmacokinetics and Efficacy
- Photochemistry and Electron Transfer Studies
- Microbial Metabolites in Food Biotechnology
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Enzyme Catalysis and Immobilization
- X-ray Diffraction in Crystallography
- Glycosylation and Glycoproteins Research
- Chinese history and philosophy
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
Sun Yat-sen University
2003-2025
Anhui Medical University
2025
Chinese PLA General Hospital
2025
The First Affiliated Hospital, Sun Yat-sen University
2025
ShanghaiTech University
2022-2024
Qilu Hospital of Shandong University
2024
Changjiang Water Resources Commission
2024
Shanghai Clinical Research Center
2024
Nankai University
2014-2023
State Key Laboratory of Medicinal Chemical Biology
2012-2023
Metallomodulation cell death strategies are extensively investigated for antitumor therapy, such as cuproptosis, ferroptosis, and chemodynamic therapy (CDT). Undoubtedly, the accurate specific elevation of metal ions levels in cancer cells is key to boosting their therapeutic index. Herein, a programmably controllable delivery system based on croconium dye (Croc)-ferrous ion (Fe2+ ) nanoprobes (CFNPs) developed multiscale dynamic imaging guided photothermal primed CDT. The Croc, with kinds...
Glucose and lactate play important roles for tumor growth. How to simultaneously deprive tumors of glucose is a big challenge. We have developed cascade catalytic system (denoted as FPGLC) based on fluorinated polymer (FP) with co-loading oxidase (GOx), (LOx), catalase (CAT). GOx LOx lactate, respectively, resulting in abundant hydrogen peroxide (H2 O2 ) generation. Meanwhile, CAT catalyzes H2 into , which not only promotes reactions consuming more but also alleviates hypoxia. Benefiting...
Abstract Conventional antibiotics are ineffective against non‐replicating bacteria (for example, within biofilms). We report a series of halogenated phenazines (HP), inspired by marine antibiotic 1 , that targets persistent bacteria. HP 14 demonstrated the most potent biofilm eradication activities to date MRSA, MRSE, and VRE biofilms (MBEC=0.2–12.5 μM), as well effective killing MRSA persister cells in non‐biofilm cultures. Frontline treatments, vancomycin daptomycin, were unable eradicate...
The development of blood-brain barrier (BBB)-crossing phototheranostic agents in second near-infrared window (NIR-II), especially the range 1500-1700 nm (NIR-IIb), affords great opportunities for glioblastoma (GBM) management. Herein, an organic assembly (denoted as LET-12) with maximum absorption peak at 1400 and emission 1512 trailing over 1700 through self-assembly small molecule IR-1064 is designed subsequently decorated choline acetylcholine analogs. LET-12 can effectively cross BBB...
Bacterial persister cells are dormant and highly tolerant to lethal antibiotics, which believed be the major cause of recurring chronic infections. Activation toxins bacterial toxin-antitoxin systems inhibits growth plays an important role in formation. However, little is known about overall gene expression profile upon toxin activation. More importantly, how persisters evade host immune clearance remains poorly understood. Here we demonstrate that a Pseudomonas aeruginosa system HigB-HigA...
Polymyxin B and E (colistin) are the last resorts to treat multidrug-resistant Gram-negative pathogens. Pseudomonas aeruginosa is intrinsically resistant a variety of antibiotics. The PhoP-PhoQ two-component regulatory system contributes resistance polymyxins by regulating an arnBCADTEF-pmrE operon that encodes lipopolysaccharide modification enzymes. To identify additional PhoP-regulated genes contribute tolerance polymyxin B, we performed chromatin immunoprecipitation sequencing (ChIP-Seq)...
Pseudomonas aeruginosa is a significant pathogen mainly causing healthcare-associated infections (HAIs). Newly emerging high-risk clones of P. with elevated virulence profiles furtherly cause severe community-acquired (CAIs). Usually, it not common for to co-carry exoU and exoS genes, encoding two type III secretion system (T3SS) effectors. The pathogenicity mechanism exoS+/exoU+ strains remains unclear. Here, we provide detailed evidence subset hypervirulent strains, which abundantly...
Bacterial biofilms are surface-attached communities of bacteria that are: (1) highly prevalent in human infections, and (2) resistant to conventional antibiotic treatments host immune responses.
Carbon metabolism plays an essential role in bacterial pathogenesis and susceptibility to antibiotics. In Pseudomonas aeruginosa, Crc, Hfq, a small RNA, CrcZ, are central regulators of carbon metabolism. By screening mutants genes involved metabolism, we found that mutation the tpiA gene reduces expression type III secretion system (T3SS) resistance aminoglycoside TpiA is triosephosphate isomerase reversibly converts glyceraldehyde 3-phosphate dihydroxyacetone phosphate, key step connecting...
During infection of a host, Pseudomonas aeruginosa orchestrates global gene expression to adapt the host environment and counter immune attacks. P. harbours hundreds regulatory genes that play essential roles in controlling expression. However, their contributions bacterial pathogenesis remain largely unknown. In this study, we analysed transcriptomic profile cells isolated from lungs infected mice examined upregulated virulence. Mutation novel pvrA (PA2957) attenuated virulence an acute...
Abstract Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. “Rescue” of viability occurs if the female harbors same strain. CI is caused by linked pairs genes called factors (CifA and CifB). The co-evolution CifA-CifB may account in part for patterns documented infected different strains, but molecular mechanisms remain elusive. Here, we use X-ray crystallography AlphaFold analyze from strain w...
Abstract Forced expression of defined transcriptional factors has been well documented as an effective method for cellular reprogramming or directed differentiation. However, transgene is not amenable therapeutic application due to potential insertional mutagenesis. Here, we have developed a bacterial type III secretion system (T3SS)-based protein delivery tool and shown its in directing pluripotent stem cell differentiation by controlled transcription relevant early heart development. By...
Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen possessing type III secretion system (T3SS) which injects toxic effector proteins into mammalian host cells. In previous studies, P. strains lacking all of the known effectors were shown to cause cytotoxicity upon prolonged infection time. this study, we report identification new cytotoxin, nucleoside diphosphate kinase (NDK), injected eukaryotic cells in T3SS-dependent manner. Injection NDK inhibited by presence...
Posttranscriptional regulation plays an essential role in the quick adaptation of pathogenic bacteria to host environments, and RNases play key roles this process by modifying small RNAs mRNAs. We find that Pseudomonas aeruginosa endonuclease YbeY is required for rRNA processing bacterial virulence a murine acute pneumonia model. Transcriptomic analyses reveal knocking out ybeY gene results downregulation oxidative stress response genes, including catalase genes katA katB Consistently,...
The evolution of bacterial antibiotic resistance is exhausting the list currently used antibiotics and endangers those in pipeline. combination a promising strategy that may suppress development and/or achieve synergistic therapeutic effects. Eravacycline newly approved effective against variety multidrug-resistant (MDR) pathogens. However, to eravacycline strategies remain unexplored. Here, we demonstrated carbapenem-resistant Klebsiella pneumoniae clinical isolate quickly developed...
Abstract Pseudomonas aeruginosa is capable of causing acute and chronic infections in various host tissues, which depends on its abilities to effectively utilize host-derived nutrients produce protein virulence factors toxic compounds. However, the regulatory mechanisms that direct metabolic intermediates towards production compounds are poorly understood. We previously identified a PvrA controls genes involved fatty acid catabolism by binding palmitoyl-coenzyme A (CoA). In this study,...
Glucagon-like peptide-1 (GLP-1) has considerable potential as a possible therapeutic agent for type-2 diabetes. Unfortunately, this glucoincretin is short lived due to degradation by dipeptidyl-peptidase IV and rapid clearance renal filtration. In study, we attempted extend GLP-1 action through the attachment of lysine residue at N-terminal (named KGLP-1), make fusion protein with human serum albumin (HSA) in Pichia pastoris. The protein, designated KGLP-1/HSA, was purified an immunomagnetic...
Agents capable of eradicating bacterial biofilms are great importance to human health as biofilm-associated infections tolerant our current antibiotic therapies. We have recently discovered that halogenated quinoline (HQ) small molecules are: 1) methicillin-resistant Staphylococcus aureus (MRSA), epidermidis (MRSE) and vancomycin-resistant Enterococcus faecium (VRE) biofilms, 2) synthetic tuning the 2-position HQ scaffold has a significant impact on antibacterial antibiofilm activities....