A5043196249- Plant-Microbe Interactions and Immunity
- Nuclear Structure and Function
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Resistance
- Plant Micronutrient Interactions and Effects
- Toxin Mechanisms and Immunotoxins
- PARP inhibition in cancer therapy
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Plant Virus Research Studies
- Trace Elements in Health
- Plant Parasitism and Resistance
- Plant Gene Expression Analysis
- Plant responses to water stress
- Hippo pathway signaling and YAP/TAZ
Leibniz Institute of Plant Biochemistry
2019-2024
Leibniz Association
2023
Freie Universität Berlin
2016-2022
Sainsbury Laboratory
2013-2018
Norwich Research Park
2011-2018
University of Cologne
2010-2016
Cluster of Excellence on Plant Sciences
2016
John Innes Centre
2010-2014
Max Planck Institute for Plant Breeding Research
2007-2013
Coordination of different defense pathways across cell compartments produces a fully effective innate immune response.
Abstract Plant immune responses depend on dynamic signaling events across the nuclear envelope through pores. Nuclear accumulation of certain resistance (R) proteins and downstream signal transducers are critical for their functions, but it is not understood how these processes controlled. Here, we report identification, cloning, analysis Arabidopsis thaliana modifier snc1,7 (mos7-1), a partial loss-of-function mutation that suppresses conditioned by autoactivated R protein snc1 (for...
The downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa) is a filamentous oomycete that invades plant cells via sophisticated but poorly understood structures called haustoria. Haustoria are separated from the host cell cytoplasm and surrounded by an extrahaustorial membrane (EHM) of unknown origin. In some interactions, including Hpa-Arabidopsis, haustoria progressively encased host-derived, callose-rich materials molecular mechanisms which callose accumulates around remain unclear....
Plants have evolved strong innate immunity mechanisms, but successful pathogens evade or suppress plant via effectors delivered into the cell. Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on Arabidopsis thaliana, and a genome sequence is available for isolate Emoy2. Here, we exploit availability of sequences Hpa to measure gene-expression changes in both simultaneously during infection. Using high-throughput cDNA tag sequencing method, reveal expression patterns predicted genes...
Although a plethora of nuclear envelope (NE) transmembrane proteins (NETs) have been identified in opisthokonts, plant NETs are largely unknown. The only known NET homologues plants Sad1/UNC-84 (SUN) proteins, which bind Klarsicht/ANC-1/Syne-1 homology (KASH) proteins. Therefore, de novo identification is necessary. Based on similarities between opisthokont KASH and the WPP domain–interacting we used computational method to identify subset NETs. Ten potential protein families were...
The oomycete pathogen Hyaloperonospora arabidopsidis (Hpa) causes downy mildew disease on Arabidopsis. To colonize its host, Hpa translocates effector proteins that suppress plant immunity into infected host cells. Here, we investigate the relevance of interaction between one these effectors, HaRxL106, and Arabidopsis RADICAL-INDUCED CELL DEATH1 (RCD1). We use infection assays as well molecular biochemical analyses to test hypothesis HaRxL106 manipulates RCD1 attenuate transcriptional...
The COP1/SPA complex acts as an E3 ubiquitin ligase to repress photomorphogenesis by targeting activators of the light response for degradation. Genetic analysis has shown that four members SPA gene family (SPA1-SPA4) have overlapping but distinct functions. In particular, SPA1 and SPA2 differ in encodes a potent repressor light- dark-grown seedlings, fully loses its function when seedlings are exposed light, indicating is hyper-inactivated light. Here, we used chimeric SPA1/SPA2 constructs...
In plant effector-triggered immunity (ETI), intracellular nucleotide binding-leucine rich repeat (NLR) receptors are activated by specific pathogen effectors. The Arabidopsis TIR (Toll Interleukin1 receptor domain)-NLR (denoted TNL) gene pair, RPS4 and RRS1, confers resistance to Pseudomonas syringae pv tomato (Pst) strain DC3000 expressing the Type III-secreted effector, AvrRps4. Nuclear accumulation of AvrRps4, TNL regulator EDS1 is necessary for ETI. RRS1 possesses a C-terminal 'WRKY'...
Nuclear translocation of immune regulatory proteins and signal transducers is an essential process in animal plant defense signaling against pathogenic microbes. Import containing a nuclear localization (NLS) into the nucleus mediated by transport receptors termed importins, typically dimers cargo-binding α-subunit β-subunit that mediates through pore complex. Here, we review recent reports importin-α cargo specificity mutant phenotypes plant- animal-microbe interactions. Using homology...
Importin-αs are essential adapter proteins that recruit cytoplasmic destined for active nuclear import to the transport machinery. Cargo interact with importin-α armadillo repeat domain via localization sequences (NLSs), short amino acids motifs enriched in Lys and Arg residues. Plant genomes typically encode several paralogs can have both specific partially redundant functions. Although some cargos preferentially imported by a distinct it remains unknown how this specificity is generated...
Pathogen-responsive mitogen-activated protein kinase (MAPK or MPK) cascades relay signals from activated immune receptors across the nuclear envelope to intranuclear targets. However, in plants, little is known about spatial control of MAPK signaling. Here, we report that Arabidopsis (Arabidopsis thaliana) pore complex Nup88/MOS7 essential for immunity necrotrophic fungus Botrytis cinerea. The mos7-1 mutation, causing a four-amino acid deletion, compromises B. cinerea-induced activation key...
SUMMARY IMPORTIN‐α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin‐α isoforms in Arabidopsis that recruit nuclear localization signal‐containing cargo proteins to the import machinery. IMP‐α3 / MOS6 required genetically for full autoimmunity nucleotide‐binding leucine‐rich repeat immune receptor mutant snc1 ( suppressor npr1‐1, constitutive 1 ) and also contributes basal disease resistance. Here, we investigated contribution other importin‐ α genes both types responses, analyzed...
Filamentous pathogens, such as oomycetes, employ multiple strategies to suppress plant immunity, one of which involves manipulating phytohormone signaling pathways. The effector HaRxL106 from Hyaloperonospora arabidopsidis (Hpa), known induce shade avoidance syndrome (SAS)-like growth, has previously been shown interact with brassinosteroid components. Here, we investigate the role in altering auxin signaling, revealing that targets AUX/IAA protein IAA11, a key negative regulator responses....
Proteins of the Poly(ADP-Ribose) Polymerase (PARP) family modify target proteins by covalent attachment ADP-ribose moieties onto amino acid side chains. In Arabidopsis, PARP contribute to repair DNA lesions and modulate plant responses various abiotic biotic stressors. Arabidopsis PARP1 PARP2 are nuclear given that their molecular weights exceed diffusion limit pore complexes, an active import mechanism into nucleus is likely. Here we use confocal microscopy fluorescent protein-tagged...
Abstract Poly(ADP-ribosyl)ation (PARylation) is a reversible post-translational protein modification that has profound regulatory functions in metabolism, development and immunity, conserved throughout the eukaryotic lineage. Contrary to metazoa, many components mechanistic details of PARylation have remained unidentified plants. Here we present transcriptional co-regulator RADICAL-INDUCED CELL DEATH1 (RCD1) as plant PAR-reader. RCD1 multidomain with intrinsically disordered regions (IDRs)...
M.-C. Caillaud1, L. Wirthmueller2, G. Fabro1,3, S.J.M. Piquerez1, S. Asai1, N. Ishaque1 and J.D.G. Jones1 1The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, NR4 7UH, United Kingdom 2John Correspondence: jonathan.jones{at}tsl.ac.uk ↵3 Present address: CIQUIBIC-CONICET, Departamento de Quimica Biologica, Facultad Ciencias Quimicas, Universidad Nacional Cordoba, Cordoba X500HUA, Argentina.
Plants have evolved complex mechanisms to adapt growth and development the light environment. The COP1/SPA is a key repressor of photomorphogenesis in dark-grown Arabidopsis plants acts as an E3 ubiquitin ligase ubiquitinate transcription factors involved response. In light, activity inhibited by photoreceptors, thereby allowing accumulation these subsequent Previous results shown that four members SPA family exhibit partially divergent functions. particular, SPA1 SPA2 strongly differ their...
Abstract Continuous reprogramming of gene expression in response to environmental signals is required for plant survival changing environment. One mechanism responsible this signaling through hub proteins that integrate external stimuli and transcriptional responses. RADICAL-INDUCED CELL DEATH1 (RCD1) functions as a nuclear protein, interacts with variety transcription factors its C-terminal RST domain acts co-regulator numerous stress responses plants. Here, previously unknown function RCD1...
Mono ADP-ribosyltransferases (mADP-RTs) are a family of enzymes that cleave NAD(+) and covalently attach the ADP-ribosyl moiety to target proteins. mADP-RTs well established as important virulence factors bacteria infect mammals. Cholera toxin, pertussis diphtheria toxin three best-known examples mADP-RTs. They modify host proteins in order promote infection and/or killing cell. Despite low sequence similarity at primary amino acid level, share conserved core catalytic fold structural...