A5000600091- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Hemophilia Treatment and Research
- Platelet Disorders and Treatments
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Antiplatelet Therapy and Cardiovascular Diseases
- Atrial Fibrillation Management and Outcomes
- Monoclonal and Polyclonal Antibodies Research
- Blood properties and coagulation
- Chronic Myeloid Leukemia Treatments
- Immunodeficiency and Autoimmune Disorders
- Heparin-Induced Thrombocytopenia and Thrombosis
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Trace Elements in Health
- Blood groups and transfusion
- Macrophage Migration Inhibitory Factor
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Microscopic Colitis
- Blood disorders and treatments
University Hospital Bonn
2016-2025
University of Bonn
2014-2025
Institute for Transfusion Medicine
2014-2025
University of North Carolina at Chapel Hill
2023
Klinik und Poliklinik für Hals-Nasen-Ohren-Heilkunde
2022
Justus-Liebig-Universität Gießen
2008
Philipps University of Marburg
2002
Freie Universität Berlin
1972-1991
University of Münster
1987
Klinik für Schlafmedizin
1986
In two multicenter trials, a total of 576 patients with acute myeloid leukemia (AML) were treated and found to be evaluable. Two hundred forty-two in 1978 pilot study 334 1982 randomized study. Ages between 15 78 years (median, 48). The uniform remission induction therapy both studies consisted one courses 9-day combination 6-thioguanine (TG) cytosine arabinoside (ARA-C) daunorubicin (DNR) [TAD9]. timing sequencing TAD9 was designed according cell kinetic effects ARA-C. A complete (CR)...
Abstract Congenital platelet disorders are rare and targeted treatment is usually not possible. Inherited function (iPFDs) can affect surface receptors multiple responses such as defects of granules, signal transduction, procoagulant activity. If iPFDs also associated with a reduced count (thrombocytopenia), it uncommon to be misdiagnosed immune thrombocytopenia. Because the bleeding tendency different variable, correct diagnosis defect based on phenotyping, analysis, genotyping essential,...
Abstract The effect of dextran sulfate on [ 3 H]dThd incorporation in lymphoid cells was investigated. polyanion activated DNA synthesis spleen and bone marrow normal mice. highest rate activation detected athymic (nude) mice; the ratio higher TxBM thymectomized, lethally irradiated protected mice than donors. Thymus could not be stimulated, but a slight response obtained cortisone‐resistant thymus cells. A synergetic found using combination phytohemagglutinin (PHA). In contrast,...
SummaryZn2+ induced DNA synthesis to a varying degree in all peripheral lymphocyte cultures from healthy adults and human cord blood. The effect of zinc acetate, aspartate, chloride, sulfate was identical, optimal stimulant concentration ranging 2.25 2.75 × 10-4M. Zinc-treated did not show more cytotoxicity than untreated controls. Zn2+ stimulated few blast cells little at 3 days, but mitotic response occurred on day 6. A long contact the with seems be necessary induce maximal
The objective of this study was to evaluate the elimination kinetics hemostasis-related biomarkers including prothrombin activation fragment F1+2, thrombin-antithrombin complex (TAT), plasmin-α2-antiplasmin (PAP), and D-dimer in humans. Autologous serum used as a biomarker source infused into 15 healthy volunteers. Serum prepared from whole blood presence recombinant tissue-type plasminogen activator (final concentration 20 μg/mL) induce plasmin generation required for PAP formation....
Summary Inhibition of thrombin by endogenous inhibitors plays a central role in the spatiotemporal control clot formation. A failure to adequately inactivate such as antithrombin deficiency generates strong prothrombotic phenotype. To study if and what extent delayed inactivation rates beyond contribute phenotype we measured inhibition profiles plasma samples obtained from 16 healthy individuals 39 thrombophilic patients, including 17 patients diagnosed positive for...
Essentials•Disorders in the breakdown of blood clots (fibrinolysis) may increase risk thrombosis.•We studied proteins involved fibrinolysis taken after stimulation clot formation.•Protein levels changed over time and did not fully normalize 8 hours.•These changes depended among others on study participants' history thrombosis.AbstractBackgroundRecently, we have shown alterations anticoagulant response to recombinant activated factor VII (rFVIIa)–induced coagulation activation patients with...
Abstract In this article, our goal is to offer an introduction and overview of the diagnostic approach inherited platelet function defects (iPFDs) for clinicians laboratory personnel who are beginning engage in field. We describe most commonly used methods propose a four-step approach, wherein each stage requires higher level expertise more specialized methods. It should be noted that proposed differs from ISTH Guidance on topic some points. The first step iPFD thorough medical history...
Background: Protein C (PC) activation on endothelial cells is a critical antithrombotic mechanism. Hereditary PC deficiency (PCD) which caused by mutations in the PROC gene, can predispose affected individuals to thrombophilia. Previous studies investigated APC generation PCD patients without including cells, are essential for physiological activation. This study aimed assess using novel cell-based assay. Methods: Plasma samples from 21 with 19 heterozygous (median level 58%) and 24 healthy...
Abstract Introduction Atypical sites for thrombosis include deep vein (DVT) of the upper extremity (UE-DVT), splanchnic (SVT), and cerebral venous sinus (CVST). In addition to specific pathogenic factors, their underlying mechanisms share similarities with typical thromboembolism (VTE), namely, DVT lower and/or pulmonary embolism, but are less understood. Methods Records unselected patients a history VTE (n = 2,011), UE-DVT 117), SVT 83), CVST 82), who were referred Institute in Bonn...
BACKGROUND: Clinical expressivity of the thrombophilic factor V Leiden (FVL) mutation is highly variable. Recently, we demonstrated an increased APC (activated protein C) response in asymptomatic FVL carriers compared with a history venous thromboembolism (VTE) after vivo coagulation activation. Here, further explored this association using recently developed ex model based on patient-specific endothelial colony-forming cells (ECFCs). METHODS: ECFCs and citrated plasma were obtained from...
Cytotoxicity is a cornerstone of immune defense, critical for combating tumors and infections. This process relies on the coordinated action granzymes pore-forming proteins, with Granzyme B (GZMB) Perforin (PRF1) being key markers most widely studied molecules pertaining to cytotoxicity. However, other human cytotoxic components remain underexplored, despite growing evidence their distinct, context-dependent roles. Natural Killer Cell Granule Protein 7 (NKG7) has recently emerged as crucial...