A5017015546- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Molecular Biology Techniques and Applications
- Bacillus and Francisella bacterial research
- Oral Health Pathology and Treatment
- Microbial Inactivation Methods
- Oral microbiology and periodontitis research
- Identification and Quantification in Food
- Animal Diversity and Health Studies
- Cancer and biochemical research
- Bee Products Chemical Analysis
- Metabolomics and Mass Spectrometry Studies
- Digital Imaging for Blood Diseases
Technological University Dublin
2019-2025
Centre for Research in Engineering Surface Technology
2017
Advanced Centre for Treatment, Research and Education in Cancer
2014
A standardised protocol for sample collection, preparation, spectral acquisition and data processing future studies of oral exfoliated cells based on Raman microspectroscopy.
Human saliva is a unique biofluid which can reflect the physiopathological state of an individual. The wide spectrum molecules present in saliva, compounded by close association salivary composition to serum metabolites, provide valuable information for clinical diagnostic applications through highly sensitive vibrational spectroscopic techniques such as Raman spectroscopy. However, nature terms collection and patient-related characteristics, be considered factors may strongly affect...
Oral cancer is one of the most common cancers worldwide. One-fifth world's oral subjects are from India and other South Asian countries. The present Raman mapping study was carried out to understand biochemical variations in normal malignant buccal mucosa. Data were acquired using WITec alpha 300R instrument 10 tumors unstained tissue sections. maps sections could resolve layers epithelium, i.e. basal, intermediate, superficial. Inflammatory, tumor, stromal regions distinctly depicted on...
Abstract This study demonstrates the efficacy of Raman micro‐spectroscopy oral cytological samples for differentiating dysplastic, potentially malignant lesions from those normal, healthy donors. Cells were collected using brush biopsy donors (n = 20) and patients attending a Dysplasia Clinic 20). Donors sampled at four different sites (buccal mucosa, tongue, alveolus, gingiva), to ensure matched normal all lesions, while patient taken clinically evident, histologically verified dysplastic...
Abstract Field cancerisation (FC) is potentially an underlying cause of poor treatment outcomes oral squamous cell carcinoma (OSCC). To explore the phenomenon using Raman microspectroscopy, brush biopsies from buccal mucosa, tongue, gingiva and alveolus healthy donors (n = 40) malignant lesions (PML) Dysplasia Clinic patients were examined. Contralateral normal samples 38) also collected patients. spectra acquired nucleus cytoplasm each cell, subjected to partial least squares‐discriminant...
The gold standard diagnosis of oral pre-cancer/cancer is an invasive biopsy followed by a histological examination, which may present psychological trauma and risk infection to patients. Moreover, multiple biopsies are often required monitor precancer lesions' progression. With the aim reducing need for biopsies, this study was undertaken identify pre-cancerous lesions using minimally brush saliva samples, analysed Raman spectroscopy.
Oral cancer has a poor prognosis of only 50% even in the light current technological advances. This may be attributed to still unmet clinical need diagnose oral pre-cancer and dysplasia. Raman spectroscopy, which can detect subtle biochemical changes, been explored for diagnosis cancer. study aims address by exploiting high amplification factor Surface Enhanced Spectroscopy (SERS) analyse saliva samples 10 healthy controls patients with Furthermore, this technique was compared conventional...
Oral cancer is the most common among Indian males, with 5-year- survival-rates of less than 50%. Efficacy Raman spectroscopic methods in non-invasive and objective diagnosis oral cancers confounding factors has already been demonstrated. The present microspectroscopic study was undertaken for in-depth site-specific analysis normal tumor tissues. 10 tumors unstained sections from 20 tissues were accrued. data 160 x 60 μm 140 sections, respectively, acquired using WITec alpha 300R equipped...
Severe radiation toxicity can continue years after the completion of radiotherapy for prostate cancer patients. Currently, it is impossible to predict before treatment which patients will experience these long-term side effects. New approaches based on vibrational spectroscopy have advantages over lymphocyte and genomic assays in terms minimal sample preparation, speed cost. A high throughput method has been developed measure Raman spectra from liquid plasma a cover glass bottomed 96 well...