A5055501342- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Dermatology and Skin Diseases
- Biochemical and Structural Characterization
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Microtubule and mitosis dynamics
- Pediatric health and respiratory diseases
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Probiotics and Fermented Foods
- Contact Dermatitis and Allergies
- Ubiquitin and proteasome pathways
- S100 Proteins and Annexins
- Lipid Membrane Structure and Behavior
- Chemokine receptors and signaling
- T-cell and B-cell Immunology
- Food Allergy and Anaphylaxis Research
- COVID-19 Impact on Reproduction
- Urticaria and Related Conditions
- Eosinophilic Disorders and Syndromes
- Nail Diseases and Treatments
- Bacteriophages and microbial interactions
University Hospital Schleswig-Holstein
2015-2024
University of Lübeck
2015-2024
Comenius University Bratislava
2022-2024
Kiel University
2007-2023
Nuvisan (Germany)
2022
Duke University
2018-2022
Clinical Research Center Kiel
2013
Universitäts Hautklinik Kiel
2010
RWTH Aachen University
1989-2008
Schott (Germany)
2007
Defensins contribute to host defense by disrupting the cytoplasmic membrane of microorganisms. This report shows that human β-defensins are also chemotactic for immature dendritic cells and memory T cells. Human β-defensin was selectively stably transfected express CCR6, a chemokine receptor preferentially expressed The β-defensin–induced chemotaxis sensitive pertussis toxin inhibited antibodies CCR6. binding iodinated LARC, ligand CCR6-transfected competitively displaced β-defensin. Thus,...
The growing public health problem of infections caused by multiresistant Gram-positive bacteria, in particularStaphylococcus aureus, prompted us to screen human epithelia for endogenous S. aureus-killing factors. A novel 5-kDa, nonhemolytic antimicrobial peptide (human μ-defensin-3, hBD-3) was isolated from lesional psoriatic scales and cloned keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum potent activity against many potentially pathogenic microbes including aureus...
Abstract A novel monocyte-derived neutrophil-activating peptide (MONAP) produced by lipopolysaccharide- and phorbol myristate acetate-stimulated human peripheral blood monocytes was purified sequential ion exchange-high performance liquid chromatography (HPLC), size exclusion HPLC, reversed phase HPLC. Biologic activities of the cytokine were monitored either an enzyme release assay or a chemotaxis assay, using neutrophils. Purified MONAP found to be homogeneous, giving single peak on...
Little is known about the defensive mechanisms induced in epithelial cells by pathogenic versus probiotic bacteria. The aim of our study was to compare bacterial strains such as Escherichia coli Nissle 1917 with nonprobiotic, and nonpathogenic bacteria respect innate defense intestinal mucosal cell. Here we report that E. strain a variety other bacteria, including lactobacilli--in contrast more than 40 different tested--strongly induce expression antimicrobial peptide human beta-defensin-2...
A potent inhibitor of human leukocyte elastase (EC 3.4.21.37) and porcine pancreatic 3.4.21.36) was purified to homogeneity from horny layers. It inhibits in a 1:1 molar ratio shows equilibrium dissociation constants 6 x 10(-10) M 1 10(-9) M, respectively. Inhibition plasmin, trypsin, alpha-chymotrypsin, cathepsin G not observed. This proved be an acid stable basic peptide with isoelectric point 9.7. The complete amino sequence appears unique 38% homology the C-terminal half...
We analyzed healthy human skin for the presence of endogenous antimicrobial proteins that might explain unusually high resistance against infections. A novel 14.5-kDa ribonuclease, termed RNase 7, was isolated from skin-derived stratum corneum. 7 exhibited potent ribonuclease activity and thus may contribute to well known skin. revealed broad spectrum many pathogenic microorganisms remarkably (lethal dose 90% < 30 nm) a vancomycin-resistant Enterococcus faecium. Molecular cloning primary...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Topical application of coal tar is one the oldest therapies for atopic dermatitis (AD), a T helper 2 (Th2) lymphocyte–mediated skin disease associated with loss-of-function mutations in barrier gene, filaggrin (FLG). Despite its longstanding clinical use and efficacy, molecular mechanism therapy unknown. Using organotypic models primary keratinocytes from AD patients controls, we found that activated aryl hydrocarbon receptor (AHR), resulting induction epidermal differentiation. AHR...
Production of inducible antimicrobial peptides offers a first and rapid defense response epithelial cells against invading microbes. Human beta-defensin-2 (hBD-2) is an peptide induced in various epithelia upon extracellular as well intracellular bacterial challenge. Nucleotide-binding oligomerization domain protein 2 (NOD2/CARD15) cytosolic involved recognition microbes by sensing peptidoglycan fragments (e.g. muramyl dipeptide). We used luciferase reporter gene for 2.3-kb hBD-2 promoter to...
Abstract Despite recent advances in understanding microbial diversity skin homeostasis, the relevance of dysbiosis inflammatory disease is poorly understood. Here we perform a comparative analysis communities coupled to global patterns cutaneous gene expression patients with atopic dermatitis or psoriasis. The microbiota analysed by 16S amplicon whole genome sequencing and transcriptome microarrays, followed integration data layers. We find that psoriasis can be classified distinct microbes,...
The epidermal growth factor receptor (EGFR) plays an important role in tissue homeostasis and tumor progression. However, cancer patients treated with EGFR inhibitors (EGFRIs) frequently develop acneiform skin toxicities, which are a strong predictor of patient's treatment response. We show that the early inflammatory infiltrate rash induced by EGFRI is dominated dendritic cells, macrophages, granulocytes, mast T cells. EGFRIs induce expression chemokines (CCL2, CCL5, CCL27, CXCL14)...
Neutrophil infiltration is a major feature in the pathogenesis of common cold, and respiratory viral infection cause asthma exacerbations. The factors regulating neutrophil influx are unknown. Interleukin-8 (IL-8) potent chemoattractant, which has been implicated several inflammatory diseases. In this study, we investigated presence IL-8 chemokine nasal aspirates asthmatic children (n = 12) whom was precipitated by proven infection. There were increased levels from during virus-induced...
Psoriasis is a T‐cell‐mediated skin autoimmune disease characterized by the aberrant activation of dermal dendritic cells (DCs) and sustained epidermal expression antimicrobial peptides. We have previously identified link between these two events showing that cathelicidin peptide LL37 has ability to trigger self‐nucleic acid mediated plasmacytoid DCs (pDCs) in psoriatic skin. Whether other cationic peptides exert similar activities unknown. By analyzing heparin‐binding HPLC fractions scales,...
Genes of the S100 fused-type protein (SFTP) family are clustered within epidermal differentiation complex and encode essential components that maintain epithelial homeostasis barrier functions. Recent genetic studies have shown mutations gene encoding SFTP filaggrin cause ichthyosis vulgaris major predisposing factors for atopic dermatitis. As a vital component healthy skin, is also precursor natural moisturizing factors. Here we present discovery member this family, designated as...