A5073406502- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Psoriasis: Treatment and Pathogenesis
- Skin Protection and Aging
- Advancements in Transdermal Drug Delivery
- Asthma and respiratory diseases
- Urticaria and Related Conditions
- Transgenic Plants and Applications
- Immunotherapy and Immune Responses
- Contact Dermatitis and Allergies
- Food Allergy and Anaphylaxis Research
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Immunotoxicology and immune responses
- Gut microbiota and health
- Skin and Cellular Biology Research
- Exercise and Physiological Responses
- Antimicrobial Peptides and Activities
- T-cell and B-cell Immunology
- Pluripotent Stem Cells Research
- Toxic Organic Pollutants Impact
- Nail Diseases and Treatments
- Cancer-related Molecular Pathways
- IL-33, ST2, and ILC Pathways
- Hedgehog Signaling Pathway Studies
Radboud University Nijmegen
2016-2025
Radboud University Medical Center
2016-2025
University Medical Center
2024-2025
Radboud Institute for Molecular Life Sciences
2015-2024
Nijmegen Institute for Scientist Practitioners in Addiction
2012
King's College London
2009
Topical application of coal tar is one the oldest therapies for atopic dermatitis (AD), a T helper 2 (Th2) lymphocyte–mediated skin disease associated with loss-of-function mutations in barrier gene, filaggrin (FLG). Despite its longstanding clinical use and efficacy, molecular mechanism therapy unknown. Using organotypic models primary keratinocytes from AD patients controls, we found that activated aryl hydrocarbon receptor (AHR), resulting induction epidermal differentiation. AHR...
Abstract Despite recent advances in understanding microbial diversity skin homeostasis, the relevance of dysbiosis inflammatory disease is poorly understood. Here we perform a comparative analysis communities coupled to global patterns cutaneous gene expression patients with atopic dermatitis or psoriasis. The microbiota analysed by 16S amplicon whole genome sequencing and transcriptome microarrays, followed integration data layers. We find that psoriasis can be classified distinct microbes,...
Abstract Background Recent advances in sequencing technologies have enabled metagenomic analyses of many human body sites. Several studies catalogued the composition bacterial communities surface skin, mostly under static conditions healthy volunteers. Skin injury will disturb cutaneous homeostasis host tissue and its commensal microbiota, but dynamics this process not been studied before. Here we analyzed microbiota layer deeper layers stratum corneum normal investigated recolonization skin...
The strong societal urge to reduce the use of experimental animals, and biological differences between rodent human skin, have led development alternative models for healthy diseased skin. However, limited availability primary keratinocytes generate such hampers large-scale implementation skin in biomedical, toxicological, pharmaceutical research. Immortalized cell lines may overcome these issues, however, few immortalized keratinocyte are available most do not form a fully stratified...
Abstract Background Interleukin (IL)-13 is a key driver of inflammation and barrier dysfunction in atopic dermatitis (AD). While there robust evidence that tralokinumab – monoclonal antibody neutralizes IL-13 reduces clinical disease activity, less known about its effects on function. Objectives To characterize the treatment skin Methods Transepidermal water loss (TEWL), stratum corneum hydration (SCH), natural moisturizing factor content, histopathological characteristics, biomarker...
Bacteria represent a promising dynamic delivery system for the treatment of disease. In skin, relevant location Cutibacterium acnes within hair follicle makes this bacterium an attractive chassis dermal biotechnological applications. Here, we provide genetic toolbox engineering traditionally intractable bacterium, including basic gene expression tools, biocontainment strategies, markerless engineering, and transcriptional regulation. As proof concept, develop antioxidant-secreting strain...
Recent studies have emphasized the importance of heritable and acquired skin barrier abnormalities in common inflammatory diseases such as psoriasis atopic dermatitis (AD). To date, no comprehensive on effect experimental disruption cornified envelope protein expression been performed.To analyse structural proteins keratinocyte differentiation-regulating proteins.We examined mRNA (day 1, 3 7) 2, 4 9) levels regulatory molecules after sodium dodecyl sulphate (SDS) application normal skin,...
Skin colonization by Staphylococcus aureus and its relative abundance is associated with atopic dermatitis (AD) disease severity treatment response. Low levels of antimicrobial peptides in AD skin may be related to the microbial dysbiosis. Therapeutic targeting microbiome peptide expression can, therefore, restore homeostasis combat AD. In this study, we analyzed cutaneous composition 7 patients 10 healthy volunteers upon topical coal tar or vehicle treatment. We implemented validated a...
Abstract The tumour microenvironment (TME) forms a major obstacle in effective cancer treatment and for clinical success of immunotherapy. Conventional co-cultures have shed light onto multiple aspects immunobiology, but they are limited by the lack physiological complexity. We develop human organotypic skin melanoma culture (OMC) that allows real-time study host-malignant cell interactions within multicellular tissue architecture. By co-culturing decellularized dermis with keratinocytes,...
Terminally differentiating epidermal keratinocytes express a large number of structural and antimicrobial proteins that are involved in the physical barrier function stratum corneum provide innate cutaneous host defense. Late cornified envelope (LCE) genes, located differentiation complex on chromosome 1, encode family 18 unknown function, whose expression is largely restricted to epidermis. Deletion two members, LCE3B LCE3C (LCE3B/C-del), widely-replicated psoriasis risk factor interacts...
Transcription factor p63 is a key regulator of epidermal keratinocyte proliferation and differentiation. Mutations in the DNA-binding domain are associated with ectrodactyly, ectodermal dysplasia, cleft lip/palate (EEC) syndrome. However, underlying molecular mechanism these mutations remains unclear. Here, we characterized transcriptome epigenome mutant keratinocytes derived from EEC patients. The deviated normal cell identity. Epigenomic analyses showed an altered enhancer landscape...
Abstract Background Chronic inflammatory skin diseases such as atopic dermatitis (AD) and psoriasis (PSO) present major challenges in health care. Thus, biomarkers to identify disease trajectories response treatments improve the lives of affected individuals warrant great research consideration. The requirements that these must fulfil for use practical clinical tools have not yet been adequately investigated. Aim To core elements high‐quality AD PSO prepare recommendations current biomarker...
The epidermal-derived "alarmins" high-mobility group box 1 (HMGB1) protein and interleukin-33 (IL-33) are upregulated in patients with atopic dermatitis. How-ever, their capacity as pro-inflammatory cytokines derived effects on skin barrier regulation poorly elucidated. We investigated the impact of HMGB1 IL-33 gene transcription, expression epidermal differentiation across 3 distinct keratinocyte vitro models. Primary keratinocytes from healthy donors were used submerged monolayer cultures,...
Psoriasis and atopic dermatitis are chronic inflammatory skin diseases characterized by keratinocyte (KC) hyperproliferation epidermal acanthosis (hyperplasia). The milieu of disease-associated cytokines soluble factors is considered a mitogenic factor; however, pinpointing the exact mitogens in this complex microenvironment challenging. We employed organotypic human equivalents, faithfully mimicking native proliferation stratification, to evaluate proliferative effects broad panel...