A5015004042- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- Retinoids in leukemia and cellular processes
- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Genetic Associations and Epidemiology
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
- Estrogen and related hormone effects
- NF-κB Signaling Pathways
- Melanoma and MAPK Pathways
- Rheumatoid Arthritis Research and Therapies
- Diabetes and associated disorders
- Spondyloarthritis Studies and Treatments
- S100 Proteins and Annexins
- Nutrition, Genetics, and Disease
- Mast cells and histamine
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Atherosclerosis and Cardiovascular Diseases
University of Michigan
2016-2025
Michigan Medicine
2007-2024
Michigan United
2011-2024
Ann Arbor VA Medical Center
2004-2023
Veterans Health Administration
2012-2023
York University
2002-2023
VA Ann Arbor Healthcare System
2002-2021
Women's College Hospital
2018
Toronto Western Hospital
2018
University of Toronto
2018
Transforming growth factor α (TGF-α) is produced by and required for the of epithelial cells angiogenic in vivo. Since epidermal hyperplasia angiogenesis are hallmarks psoriasis, TGF-α gene expression was analyzed biopsies normal psoriatic skin. messenger RNA protein much more abundant lesional epidermis than normal-appearing skin patients or epidermis. In contrast, levels transforming β1 (TGF-β1), which inhibits cell growth, not significantly different normal, uninvolved, Thus, may involve...
Abstract Th1 and Th17 T cells are often colocalized in pathological environments, yet Th1-derived IFN-γ inhibits cell development vitro. We explored the physiologic basis of this paradox humans. In study, we demonstrate increased number CD4+ CD8+ IL-17+ skin lesions psoriasis. Furthermore, show that myeloid APCs potently support induction cells, activity is greatly tested stimuli might account for activity. psoriatic blood lesional skin. programs to induce human via IL-1 IL-23. also...
The DNase I-hypersensitive sites in the human embryonic beta-globin gene region have been mapped erythroid-enriched fractions of disaggregated fetal livers, adult nucleated red blood cells, and brain tissue. Our analysis a extending 11 kilobases (kb) 5' epsilon-globin reveals many minor nuclease-hypersensitive one major site located 6.1 kb upstream gene. All these hypersensitive are erythroid-specific, is stable throughout erythroid development. As assayed by nuclear runoff transcription,...
IL-1F6, IL-1F8, and IL-1F9 the IL-1R6(RP2) receptor antagonist IL-1F5 constitute a novel IL-1 signaling system that is poorly characterized in skin. To further characterize these cytokines healthy inflamed skin, we studied their expression control, uninvolved psoriasis, psoriasis plaque skin using quantitative RT-PCR immunohistochemistry. Expression of IL-1F5, -1F6, -1F8, -1F9 were increased 2 to 3 orders magnitude versus which was supported immunohistologically. Moreover, treatment with...
Although analysis pipelines have been developed to use RNA-seq identify long non-coding RNAs (lncRNAs), inference of their biological and pathological relevance remains a challenge. As result, most transcriptome studies autoimmune disease only assessed protein-coding transcripts.We used data from 99 lesional psoriatic, 27 uninvolved 90 normal skin biopsies, applied computational approaches characterize expressed lncRNAs. We detect 2,942 previously annotated 1,080 novel lncRNAs which are...
Objective Skin inflammation and photosensitivity are common in patients with cutaneous lupus erythematosus (CLE) systemic (SLE), yet little is known about the mechanisms that regulate these traits. Here we investigate role of interferon kappa (IFN-κ) regulation type I (IFN) photosensitive responses examine its dysregulation skin. Methods mRNA expression IFN genes was analysed from microarray data CLE lesions healthy control Similar cultured primary keratinocytes, fibroblasts endothelial...
Individuals with psychiatric disorders have elevated rates of autoimmune comorbidity and altered immune signaling. It is unclear whether these immunological states a shared genetic basis those disorders. The present study sought to use existing summary-level data from previous genome-wide association studies determine if commonly varying single nucleotide polymorphisms are between immune-related phenotypes. We estimated heritability examined pair-wise correlations using the linkage...
C-reactive protein (CRP) is associated with immune, cardiometabolic, and psychiatric traits diseases. Yet it inconclusive whether these associations are causal.We performed Mendelian randomization (MR) analyses using two genetic risk scores (GRSs) as instrumental variables (IVs). The first GRS consisted of four single nucleotide polymorphisms (SNPs) in the CRP gene (GRSCRP), second 18 SNPs that were significantly levels largest genome-wide association study (GWAS) to date (GRSGWAS). To...
Abstract The immunopathogenesis of psoriasis, a common chronic inflammatory disease the skin, is incompletely understood. Here we demonstrate, using combination single cell and spatial RNA sequencing, IL-36 dependent amplification IL-17A TNF responses in absence neutrophil proteases, which primarily occur within supraspinous layer psoriatic epidermis. We further show that subset SFRP2 + fibroblasts psoriasis contribute to immune network through transition pro-inflammatory state. fibroblast...
In this report, we investigated BRAF/NRAS mutations in samples from a case–control study of melanoma and series benign melanocytic nevi. We evaluated potential associations between BRAF histopathologic pigmentary characteristics melanoma. Mutations NRAS were detected by sequencing microdissected/laser-captured DNA 18 in-situ melanomas, 64 primary 51 Nevi showed the highest frequency (82%). identified 29% invasive melanomas only 5.6% melanomas. found 5.2% 5.9% nevi no seen A majority observed...
beta globin gene fragments from a patient with homozygous beta+-thalassemia have been cloned and subjected to restriction endonuclease, nucleotide sequence, in vitro trancription analyses. Restriction endonuclease mapping of the revealed no deletions or other rearrangements, transcription thalassemic appeared be normal vitro. However, sequence analysis beta+-thalassemic permitted identification single base change body small intervening sequence. This creates much like that 3' splice site The...
Obesity is a significant risk factor for psoriasis and body mass index (BMI) correlates with disease severity. Objectives To investigate the relationship between obesity psoriasis, focusing on role of adipokines such as leptin resistin.Patients (n = 30) were recruited their BMI, waist circumference severity [Psoriasis Area Severity Index (PASI)] recorded. Fasting serum samples obtained enrolment after course ultraviolet (UV) B treatment. Age-, sex- BMI-matched healthy controls also...