A5013582491- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- TGF-β signaling in diseases
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Galectins and Cancer Biology
- Platelet Disorders and Treatments
- Blood disorders and treatments
- Chemokine receptors and signaling
- Respiratory Support and Mechanisms
- Asthma and respiratory diseases
- Atherosclerosis and Cardiovascular Diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Acute Myeloid Leukemia Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pediatric health and respiratory diseases
- Liver physiology and pathology
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Academy of Athens
2013-2024
Biomedical Research Foundation of the Academy of Athens
2008-2022
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2022
Lund University
2001-2005
Umeå University
1989-2005
AstraZeneca (Sweden)
2001-2005
Ludwig Cancer Research
2001-2002
Democritus University of Thrace
2000-2001
AstraZeneca (United Kingdom)
2000
Howard Hughes Medical Institute
1998
Only mature B lymphocytes can enter the lymphoid follicles of spleen and lymph nodes thus efficiently participate in immune response. Mature, long-lived derive from short-lived precursors generated bone marrow. We show that selection into pool is an active process takes place spleen. Two populations splenic cells were identified as for cells. Transitional type 1 (T1) are recent immigrants They develop transitional 2 (T2), which cycling found exclusively primary Mature be T1 or T2
Mice that bear the X-linked immunodeficiency ( xid ) mutation have a B lymphocyte-specific defect resulting in an inability to make antibody responses polysaccharide antigens. A backcross of 1114 progeny revealed colocalization with Bruton's agammaglobulinemia tyrosine kinase btk gene, which is implicated human immune deficiency, agammaglobulinemia. carry missense alters highly conserved arginine near amino-terminus protein, Btk. Because this region Btk lies outside any obvious domain, may...
Abstract Neutrophils and complement are key sentinels of innate immunity mediators acute inflammation. Recent studies have suggested that inflammatory processes modulate thrombogenic pathways. To date, the potential cross-talk between thrombosis precise molecular pathway by which neutrophils trigger coagulation process remained elusive. In this study, we demonstrate antiphospholipid Ab-induced activation downstream signaling via C5a receptors in leads to induction tissue factor (TF), a...
Bone morphogenetic proteins (BMPs) are multifunctional that regulate the proliferation, differentiation, and migration of a large variety cell types. Like other members transforming growth factor-beta family, BMPs elicit their cellular effects through activating specific combinations type I II serine/threonine kinase receptors downstream effector proteins, which termed Smads. In present study, we investigated BMP receptor/Smad expression signaling in endothelial cells (ECs) examined on EC...
IL-28 (IFN-λ) cytokines exhibit potent antiviral and antitumor function but their full spectrum of activities remains largely unknown. Recently, cytokine family members were found to be profoundly down-regulated in allergic asthma. We now reveal a novel role inducing type 1 immunity protection from airway disease. Treatment wild-type mice with recombinant or adenovirally expressed IL-28A ameliorated disease, suppressed Th2 Th17 responses induced IFN-γ. Moreover, abrogation endogenous...
Many studies have shown that the microanatomic organization of infiltrating leukocytes in salivary gland lesions patients with Sjögren's syndrome (SS) resembles structure lymphoid organs. A newly defined set chemokines referred to as "lymphoid," which orchestrate leukocyte microenvironmental homing and contribute formation structures, provides directional clues. The aim this study was investigate possible existence "lymphoid" chronic inflammatory SS thus validate their potential involvement...
Genes were isolated using the suppression subtractive hybridization method by stimulation of pro/pre B cells with anti-CD40 and interleukin (IL)-4 to mature S mu-Sepsilon-switched cells. One strongly upregulated genes encodes a novel murine CC chemokine we have named ABCD-1. The ABCD-1 gene has three exons separated 1. 2- 2.7-kb introns. It gives rise 2.2-kb transcript containing an open reading frame 276 nucleotides. Two polyadenylation sites are used, giving cDNAs either 1550 or 1850 bp 3'...
Transcription of germ-line immunoglobulin heavy chain genes conditions them to participate in isotype switch recombination. Transforming growth factor-beta1 (TGF-beta1) stimulates promoter elements located upstream the IgA1 and IgA2 regions, designated Ialpha1 Ialpha2, contributes development IgA responses. We demonstrate that intracellular Smad proteins mediate activation by TGF-beta. TGF-beta type 1 receptor (ALK-5), activin IB (ALK-4), "orphan" ALK-7 trans-activate promoter, thus raising...
Immunoglobulin (Ig) class switching appears to be preceded by induction of germ-line transcripts. In this report, we demonstrate that transforming growth factor β (TGF-β) induces transcripts both the IgA subclasses (lgA1 and lgA2) in Branhamella catarrhalls (BC)-activated human spleen B cells. Two bands, one -1.85 kb other ∼1.6 kb, could seen cultures treated with TGF-β. The ∼ 1.85 band contains mRNA for a transcript membrane form. This co-migrates productive secreted form α mRNA. other,...
Rationale: Toll-like receptor (TLR) 7/8 ligands are promising candidate drugs for the treatment of allergic asthma and rhinitis. Although their clinical application depends on development strategies topical administration to lung, this has not been explored in preclinical disease models.Objectives: To examine therapeutic effectiveness, persistence effect, mode action intranasal TLR7 ligand airway disease.Methods: Wild-type, IFN-α (IFN-αR)−/−, IFN-γ−/−, CD8−/−, TLR7−/−, radiation-induced...
Little is known about what drives the appearance of lymphoid follicles (LFs), which may function as organs in chronic obstructive pulmonary disease (COPD). In animal infection models, LF formation requires expression homeostatic chemokines by stromal cells and dendritic cells, partly via lymphotoxin.To study role COPD to identify mechanism(s) responsible for their production.Peripheral lung chemokine lymphotoxin were visualized immunostainings quantified ELISA/quantitative reverse...
TGF-β signaling regulates a variety of cellular processes, including proliferation, apoptosis, differentiation, immune responses, and fibrogenesis. Here, we describe lysine methylation-mediated mechanism that controls the pro-fibrogenic activity TGF-β. We find methyltransferase Set9 potentiates by targeting Smad7, an inhibitory downstream effector. Smad7 methylation promotes interaction with E3 ligase Arkadia and, thus, ubiquitination-dependent degradation. Depletion or pharmacological...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Abstract Btk is a cytoplasmic protein tyrosine kinase (PTK) that has been directly implicated in the pathogenesis of X-linked agammaglobulinaemia (XLA) humans and immunodeficiency (Xid) mice. We have isolated phage cosmid clones allowed us to deduce genomic structure mouse human loci. The genes are contained within regions span approximately 43.5 kb 37.5 kb, respectively. Both loci contain 18 coding exons ranging between 55 560 bp size with introns from 164 9 kb. 5'-untranslated encoded by...
Signaling by Bone Morphogenetic Proteins (BMP) has been implicated in early lung development, adult homeostasis and tissue-injury repair. However, the precise mechanism of action spatio-temporal pattern BMP-signaling during these processes remains inadequately described. To address this, we have utilized a transgenic line harboring BMP-responsive eGFP-reporter allele (BRE-eGFP) to construct first detailed spatiotemporal map canonical BMP-pathway activation adult-lung injury We demonstrate...