A5013059636- Inflammatory Bowel Disease
- Gut microbiota and health
- IL-33, ST2, and ILC Pathways
- Immunodeficiency and Autoimmune Disorders
- Cancer Cells and Metastasis
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Cytokine Signaling Pathways and Interactions
- Cancer Immunotherapy and Biomarkers
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Chronic Lymphocytic Leukemia Research
- Orthopedic Infections and Treatments
- Total Knee Arthroplasty Outcomes
- Cancer Research and Treatments
- Esophageal and GI Pathology
- Cystic Fibrosis Research Advances
- Hormonal Regulation and Hypertension
- Reproductive System and Pregnancy
- Pharmaceutical studies and practices
- Blood disorders and treatments
- Cell Adhesion Molecules Research
- Probiotics and Fermented Foods
- Regulation of Appetite and Obesity
- Pharmacogenetics and Drug Metabolism
- Blood transfusion and management
Democritus University of Thrace
2015-2024
Hellenic Mediterranean University
2024
University Hospital of Alexandroupolis
2018-2023
General Hospital of Athens G. Genimatas
2010
Umeå University
2000
Transcription of germ-line immunoglobulin heavy chain genes conditions them to participate in isotype switch recombination. Transforming growth factor-beta1 (TGF-beta1) stimulates promoter elements located upstream the IgA1 and IgA2 regions, designated Ialpha1 Ialpha2, contributes development IgA responses. We demonstrate that intracellular Smad proteins mediate activation by TGF-beta. TGF-beta type 1 receptor (ALK-5), activin IB (ALK-4), "orphan" ALK-7 trans-activate promoter, thus raising...
The aim of the present study was to determine prevalence most common allelic variants polymorphic cytochrome P450 (CYP) enzymes CYP2D6, CYP2C9, CYP2C19 and CYP3A5 predict genotype frequency for each polymorphism in Greek population. DNA isolated from peripheral blood samples derived 283 non-related ethnic subjects used CYP2D6*3, CYP2D6*4, CYP2C9*2, CYP2C9*3 CYP3A5*3 by polymerase chain reaction (PCR)-restriction fragment length method, CYP2C19*2 CYP2C19*3 with specific amplification...
Even though anti-TNF therapy significantly improves the rates of remission in inflammatory bowel disease (IBD) patients, there is a noticeable subgroup patients who do not respond to treatment. Dysbiosis emerges as key factor IBD pathogenesis. The aim present study profile changes gut microbiome and transcriptome before after administration agent Infliximab (IFX) investigate their potential predict patient response IFX at baseline. Mucosal biopsy samples from 20 nine healthy controls (HC)...
The probiotics Lactiplantibacillus plantarum UBLP-40, Lactobacillus rhamnosus UBLR-58 and Bifidobacterium longum UBBL-64 seem to promote wound healing when applied topically. Our aim was investigate their effect on the mRNA expression of pro-inflammatory, angiogenetic factors during process a standardized excisional model in rats. Rats subjected six dorsal skin wounds were allocated Control; L. plantarum; combined formula plus B. longum; rhamnosus; treatments, every two days, along with...
Introduction: CYP2C19*17 is a novel variant allele causing ultrarapid metabolism of CYP2C19 substrates. In the present study we investigated allelic frequency and recalculated previously reported frequencies CYP2C19*1/*1 genotype all genotype-derived phenotypes for in Greek population. Materials & methods: A total 283 nonrelated healthy ethnic subjects that had already been genotyped CYP2C19*2 *3 alleles as well CYP2D6 CYP2C9 participated study. The was by PCR-RFLP method. Results: 19.61%....
Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (-786T >C and 894G >T) enhance endo-thelial dysfunction have been studied relation to coronary artery disease (CAD). In present study, we examined association of above polymorphisms with CAD, as well myocardial infarction (MI), hypertension, diabetes smoking CAD patients. Study subjects consisted 154 consecutive bypass graft (CABG) patients 155 non-CAD controls. eNOS -786T >T were genotyped by polymerase chain...
Bifidobacterium lactis, Lactobacillus acidophilus, Lactiplantibacillus plantarum and Saccharomyces boulardii are common probiotic supplements. Colonic subepithelial myofibroblasts (cSEMFs) actively involved in mucosal wound healing inflammation. cSEMFs, isolated from healthy individuals, were stimulated with 102 or 104 cfu/mL of these strains alone combination, their effect on chemokine factor expression was assessed by qRT-PCR, ELISA Sircol Assay, cSEMFs migration, Wound Healing Assay....
Metabolites produced by dysbiotic intestinal microbiota can influence disease pathophysiology participating in ligand–receptor interactions. Our aim was to investigate the differential expression of metabolite receptor (MR) genes between inflammatory bowel (IBD), healthy individuals (HIs), and controls order identify possible interactions with fibrotic pathways intestine. RNA-sequencing datasets containing 643 Crohn’s (CD) patients, 467 ulcerative colitis (UC) patients 295 HIs, 4...
Colonic subepithelial myofibroblasts (cSEMFs) are mesenchymal cells with a pivotal role in the pathophysiology of Crohn's disease (CD) fibrosis. Here, we demonstrate for first time complete expression mapping cytokine receptors, implicated inflammatory bowel diseases, primary human cSEMFs and how pro-inflammatory cytokines regulate this expression. Furthermore, show effect Th1-, Th2-, Th17- Treg-related on fibrosis-related phenotype cSEMFs.Colonic were isolated from healthy individuals'...
Surgery provokes inflammatory and immune responses, so efforts have been made to reduce host response by using less invasive techniques. The purpose of this experimental study was investigate the surgical stress induced skin incision role liver in process.Seventy male anesthetized Wistar rats were subjected a midline confined strictly (dermis) either 1 cm long (n = 20), 10 or no 20) served as controls 10). Skin trauma left open for 20-minutes period, then meticulously sutured. At 3 24 hours...
Inflammatory Bowel Diseases (IBDs) are characterized by chronic intestinal inflammation and fibrosis, the latter being predominant denominator for long-term complications. Epithelial mesenchymal 2D cultures highly utilized in vitro models preclinical evaluation of anti-inflammatory antifibrotic therapies. More recently, human organoids (HIOs), a new 3D model derived from pluripotent stem cells, have advantage to closely resemble architecture mucosa. However, appropriate timing study...
Circulating cell-free DNA (ccfDNA) of mitochondrial origin (ccf-mtDNA) consists a minor fraction total ccfDNA in blood or other biological fluids. Aberrant levels ccf-mtDNA have been observed many pathologies. Here, we introduce simple and effective standardized Taqman probe-based dual-qPCR assay for the simultaneous detection relative quantification nuclear fragments ccfDNA. Three pathologies major burden, one malignancy (Breast Cancer, BrCa), inflammatory (Osteoarthritis, OA) metabolic...
Microflora dysbiosis is implicated in the pathophysiology of Crohn’s disease. This work analyzes differences microbial communities and relevant metabolic pathways among nonstricturing nonpenetrating (B1), stricturing (B2), penetrating (B3) subphenotypes disease vs healthy controls. We conducted a bioinformatics analysis using QIIME pipeline Calypso, linear discriminant effect size, Phylogenetic Investigation Communities by Reconstruction Unobserved States, STAMP tools on publicly available...
Summary. Chronic myeloid leukaemia (CML) is a malignant clonal disorder of the haematopoietic stem cell. Treatment CML patients with interferon alpha (IFN‐α) has induced haematological and cytogenetic remission. Interferons transcriptionally activate target genes through JAK–STAT regulated factors (IRFs) family pathways. Interferon factor‐1 (IRF‐1) transcriptional activator critical for cell growth, differentiation apoptosis. The skipping exons 2 or 3 IRF‐1 in myelodysplastic syndromes acute...
Septic shock has been found to disrupt circadian rhythms. Moreover, timing of onset associated with different profiles in experimental studies.In this prospective study, we enrolled 26 patients divided into two groups: Group A (N = 15) included subjects who had septic at the time ICU admission and B 11) developed during admission. 6-Sulfatoxymelatonin (aMT6s) cortisol levels were measured urine samples every 4 h over a 24-h period. Two sets taken from (entry/septic exit) three (entry, exit)....
Oncostatin-M (OSM) is associated with antitumor necrosis factor (anti-TNF)-α resistance in inflammatory bowel disease (IBD) and fibrosis diseases. We studied the expression of OSM its receptors (OSMR, gp130) on intestinal subepithelial myofibroblasts (SEMFs) effect stimulation SEMFs.
Patients with caustic substance ingestion are usually referred to surgery departments where endoscopic evaluation is the first step towards appropriate treatment. The aim of this study was evaluate safety and efficacy conservative management in a pediatric department setting following standard protocol including endoscopy selected cases treatment based on clinical criteria. In single center observational study, all children admitted for over an 8-year-period were managed according that...
Candidates for coronary artery bypass grafting (CABG) represent a group of patients with well documented, severe disease (CAD). Genetic polymorphisms renin-angiotensin-aldosterone system (RAAS) components have been associated CAD. We examined the association angiotensin-converting enzyme (ACE), angiotensinogen (AGT), and angiotensin II type 1 receptor (AT(1) receptor) CAD in CABG patients.One hundred fifty-four 155 non-CAD controls were included study. Established PCR methods used genotyping...