Colonization of trunk neural crest derivatives in avians follows a ventral to dorsal order beginning with sympathetic ganglia, Schwann cells, sensory ganglia and finally melanocytes. Continuous emigration underlies this process, which is accounted for by progressive relocation tube progenitors prior departure. This causes gradual narrowing FoxD3, Sox9 Snail2 expression domains the that characterize these genes are no longer transcribed time melanoblasts begin emigrating. Consistently, final...

Understanding when and how multipotent progenitors segregate into diverse fates is a key question during embryonic development. The neural crest (NC) an exemplary model system with which to investigate the dynamics of progenitor cell specification, as it generates multitude derivatives. Based on 'in ovo' lineage analysis, we previously suggested early fate restriction premigratory trunk NC generate versus melanogenic fates, yet timing segregation underlying mechanisms remained unknown....

The neural crest is a migratory, multipotent cell lineage that contributes to myriad tissues, including sensory neurons and glia of the dorsal root ganglia (DRG). To identify genes affecting fate specification in crest, we performed forward genetic screen for mutations causing DRG deficiencies zebrafish. This yielded mutant lacking all DRG, which named deprived (sdp). We identified total four alleles sdp, possess lesions gene coding reversion-inducing cysteine-rich protein containing Kazal...

The neural crest (NC) is a transient embryonic structure unique to vertebrates, which generates peripheral sensory and autonomic neurons, glia, neuroendocrine chromaffin thyroid C-cells, melanocytes, mesenchymal derivatives such as parts of the skull, heart, meninges. sympathoadrenal (SA) cell lineage one major sub-lineage NC that gives rise sympathetic cells, intermediate small intensely fluorescent (SIF) cells. A key question when during ontogeny do multipotent progenitors segregate into...

Glycation, and the resulting buildup of advanced glycation end products (AGEs), is recognized as a key driver cumulative skin damage aging. Dunaliella salina halophile microalga adapted to intense solar radiation through production carotenoids. We present natural supercritical CO2 extract rich in colorless carotenoids phytoene phytofluene. The exhibited antiglycation anti-inflammatory activities ex vivo testing, showing strongly reduced formation N-ε-carboxy-methyl-lysine with exposure...

The pectoral fins of teleost fish are analogous structures to human forelimbs, and the developmental mechanisms directing their initial growth patterning conserved between tetrapods. forelimb vasculature is crucial for limb function, it appears play important roles during development by promoting other structures, but steps leading its formation poorly understood. In this study, we use high-resolution imaging document stepwise assembly zebrafish fin vasculature. We show that vascular network...

The assembly of a functional vascular system requires coordinated and dynamic transition from activation to maturation. High endothelial growth factor activity promotes activation, including junction destabilization cell motility. Maturation involves junctional stabilization formation barrier. identity mechanism action prostabilization signals are still mostly unknown. Bone morphogenetic protein receptors their ligands have important functions during embryonic vessel Previous work has...

The colorless carotenoids phytoene and phytofluene are comparatively understudied compounds found in common foods (e.g., tomatoes) human plasma, internal tissues, skin. Being naturally present foods, their intake at dietary levels is not expected to a safety concern. However, since the interest these context of many applications expanding, it important conduct studies aimed assessing safety. We here results vitro cytotoxicity genotoxicity studies, revealing no significant cytotoxic or...

Background: Next-generation 5G communication technology involves increasing use of 3–100 GHz wireless bands in population centers. Though still non-ionizing, this implies higher radiation energy vs. existing bands. The range is also shorter, needing more numerous emitters, closer to the user—resulting electromagnetic exposure. With no universal consensus regarding exposure risks, there some concern among public and scientific community, following indications that can impact immune function,...

Skin microbiota, and its diversity balance, play a key role in skin health beauty, influencing moisture, barrier function, radiance. A healthy microbiota limits the growth of detrimental species, protecting from pathologies. Prebiotics can support beneficial populations outcompeting ones. Dragon fruit (Hylocereus undatus) contains prebiotic polysaccharides effective on gut bacteria. Its extract was tested vitro, coculture model including representative double-blind, placebo-controlled...

Psychological stress exerts its effects mainly through the release of corticotropin releasing hormone (CRH), which activates inflammatory pathways in skin (inter alia), resulting redness, extracellular matrix degradation, loss elasticity and firmness, appearance wrinkles—namely, accelerated aging. In order to propose a solution this neurogenic aging phenomenon, we report here on studies using myricitrin-rich extract Cistus incanus, Mediterranean shrub used traditional medicine for treatment...

ABSTRACT The pectoral fins of teleost fish are analogous structures to human forelimbs, and the developmental mechanisms directing their initial growth patterning conserved between tetrapods. forelimb vasculature is critical for limb function, it appears play important roles during development by promoting other structures, but steps leading its formation poorly understood. In this study, we use high-resolution imaging document stepwise assembly zebrafish fin vasculature. We show that...

Effective moisture retention and a radiant luminescent skin tone are key markers of healthy beautiful skin. In recent years, accumulating data has suggested that the microbiome present on can play role in providing these benefits to particular, balance between beneficial detrimental bacteria. skin, is tipped