A5059372862- Aquaculture disease management and microbiota
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Myxozoan Parasites in Aquatic Species
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Viral gastroenteritis research and epidemiology
- Lung Cancer Treatments and Mutations
- Herpesvirus Infections and Treatments
- Circular RNAs in diseases
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- RNA modifications and cancer
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Infections and bacterial resistance
- Biosensors and Analytical Detection
- Immune Cell Function and Interaction
- Pharmaceutical and Antibiotic Environmental Impacts
Chinese Academy of Fishery Sciences
2011-2024
Shandong Center for Disease Control and Prevention
2024
Hainan Provincial Hospital of Traditional Chinese Medicine
2023
Nanjing Medical University
2021
117th Hospital of People's Liberation Army
2020
Xi'an Chest Hospital
2017-2018
Hefei Infectious Disease Hospital
2018
Nantong University
2012-2017
Affiliated Hospital of Nantong University
2012-2017
Zhongda Hospital Southeast University
2017
A novel fish reovirus, Hubei grass carp disease reovirus (HGDRV; formerly strain 104, GCRV104), was isolated from diseased in China 2009 and the full genome sequence determined. This propagated a kidney cell line with typical cytopathic effect. The total size of 23 706 bp 51 mol% G+C content, 11 dsRNA segments encoded 12 proteins (two by segment 11). nucleotide similarity search using blastn found no significant matches except for 2, which partially matched that RNA-dependent RNA polymerase...
Two strains of epidemic hemorrhagic fever (EHF) virus were isolated from the lung tissues Apodemus agrarius mice that captured in an area where EHF is endemic. The by passages A. a nonendemic area. Identification isolates usual procedures was confirmed repeated blind tests with coded sera. Contamination certain known viruses such as reovirus, adenovirus (types 3 and 7), other pathogens, murine typhus rickettsiae Leptospira, which may be naturally present wild rodents, appeared to have been...
Background: Rheumatoid arthritis (RA) is a systematic autoimmune disease which may lead to joint dysfunction and disability. Aberrant migration invasion of fibroblast-like synoviocytes (FLSs) one the most predominant etiopathogenesis RA. Quercetin bioflavonoid implicated in development RA, yet its role regulating FLSs still elusive. The aim this study investigate impact quercetin treatment on underlying mechanism.Methods: Capacity were assessed using transwell assay. Immunofluorescence assay...
Herein, we isolated the pathogenic strain ZZ051 from hemorrhagic channel catfish (Ictalurus punctatus). Physiological and biochemical identification, 16S rRNA gene sequence analysis, MALDI-TOF-MS showed that was Aeromonas veronii. After artificial infection, diseased fish symptoms similar to natural disease, characteristics of bacteria reisolated tissues were same as those original indicating pathogen responsible for disease. The isolate highly sensitive enrofloxacin but resistant...
Elizabethkingia miricola (E. miricola) is a significant pathogen that causes the crooked head disease in black spotted frogs. This has plagued numerous frog farms China and resulted substantial losses to farming industry. Nonetheless, exact mechanism frogs remains unknown. In this study, transcriptomic microbiomic analyses were conducted analyze samples infected with E. reveal infection of pathogen. Liver analysis indicated livers had 1469 differentially expressed genes when compared an...
A rapid and sensitive loop-mediated isothermal amplification (LAMP) assay for Cyprinid herpesvirus 2 (CyHV-2) detection in gibel carp was developed. Following cloning sequencing of the putative DNA helicase gene CyHV-2 isolate from China, a set four specific primers designed based on sequence. The MgCl2 concentration reaction temperature were optimized to 6 mM, 64°C, respectively. LAMP products detected by visual inspection color change due addition SYBR Green I stain. specificity...
This study aims to investigate the expression level of long non-coding RNA (lncRNA) SNHG20 in laryngeal squamous cell carcinoma (LSCC), and explore further whether it can promote development LSCC by regulating microRNA-140 (miR-140).Expression levels 56 pairs tissues adjacent normal were measured quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between with pathological parameters prognosis was analyzed. Besides, cells also analyzed qRT-PCR. knockdown...
Interactions between host and pathogen are involving various dynamic changes in transcript expression critical for understanding immunity against infections its associated pathogenesis. Herein, we established a model of channel catfish infected with Aeromonas veronii. The fish had prominent body surface bleeding, the spleen showed hyperemia swelling. Then, A. veronii was analyzed by dual RNA sequencing (RNA-seq), transcriptome data were compared uninfected or bacteria cultured vitro. profile...
Chemoresistance is one of the obstacles for effective treatment cancers, and recent evidence has shown that microRNAs play critical roles in drug resistance. In this study, we investigated effect miR-377 on cisplatin resistance osteosarcoma (OS).Tumor tissues from chemoresistant control OS patients were subjected to Real-time polymerase chain reaction (PCR) assess expression. The mechanism assessed using Cell Counting Kit (CCK) 8, flow cytometry, Western blot, luciferase assays...
To uncover the role of UPF1 in alleviating progression glioma via targeting long non-coding RNA (lncRNA) CYTOR and underlying mechanism.A total 30 tissues surgically resected from patients brain were collected trauma undergoing craniotomy during same period. Relative levels detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Correlation between was assessed, regulatory effects UPF1/CYTOR on proliferative invasive abilities U87 LN229 cells evaluated MTT...
The aim of this study was to investigate the effects retinoid X receptor α (RXRα) on proliferation and apoptosis pancreatic cancer cells through transforming growth factor-β (TGF-β)/Smad signaling pathway.The expression RXRα in tissues para-carcinoma detected via immunohistochemistry. Human PANC-1 were cultured treated with vitro. rate flow cytometry. Furthermore, changes protein level TGF-β/Smad pathway Western blotting.The significantly higher than that tissues. promoted inhibited cells....
The pharmacokinetics and tissue residues of moroxydine hydrochloride were studied in gibel carp at water temperature 15 25 °C. Samples (blood, skin, muscle, liver, kidney) collected over 10 days after the treatment analyzed by high-performance liquid chromatography with an ultraviolet detector. results indicated that influence on metabolism drug was significant. plasma concentration-time data conformed to single-compartment open model two temperatures. There higher absorption rate (t1/2ka )...