A5005675276- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Forest ecology and management
- Peptidase Inhibition and Analysis
- Studies on Chitinases and Chitosanases
- Ferroelectric and Piezoelectric Materials
- Semiconductor Lasers and Optical Devices
- Plant Water Relations and Carbon Dynamics
- Robotics and Sensor-Based Localization
- Forest Ecology and Conservation
- Microbial Metabolites in Food Biotechnology
- Photonic and Optical Devices
- Neuropeptides and Animal Physiology
- Tree-ring climate responses
- Advanced Measurement and Detection Methods
- Electrowetting and Microfluidic Technologies
- Enzyme Catalysis and Immobilization
- Analytical Chemistry and Sensors
- Advanced optical system design
- Ecology and Vegetation Dynamics Studies
- Advanced Fiber Optic Sensors
- Fire effects on ecosystems
- Tree Root and Stability Studies
National Institute of Advanced Industrial Science and Technology
2004-2022
Waseda University
1996-2021
Nagaoka University of Technology
2007-2018
Photonics Electronics Technology Research Association
2012
Chiba University
2011
Nagaoka University
2005-2009
Intelligent Systems Research (United States)
2006
Systems Research Institute
2006
Tokyo Institute of Technology
1978-2003
Tokyo Denki University
2002
ABSTRACT For direct and efficient ethanol production from cellulosic materials, we constructed a novel cellulose-degrading yeast strain by genetically codisplaying two cellulolytic enzymes on the cell surface of Saccharomyces cerevisiae . By using engineering system based α-agglutinin, endoglucanase II (EGII) filamentous fungus Trichoderma reesei QM9414 was displayed as fusion protein containing an RGSHis6 (Arg-Gly-Ser-His 6 ) peptide tag in N-terminal region. EGII activity detected pellet...
To develop a Trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, recombinant T. strain, X3AB1, was constructed that expressed an Aspergillus aculeatus β-glucosidase 1 with high specific activity under control xyn3 promoter. The culture supernatant from X3AB1 grown on 1% Avicel as carbon source had 63- and 25-fold higher against cellobiose compared to parent PC-3-7 expressing endogenous I, respectively. Further, xylanase 30% lower than due absence...
This letter reports on the formation of a layered film structure and highly improved photovoltaic output lead lanthanum zirconate titanate (PLZT) employed. The current PLZT per unit width was more than 102 times larger that bulk PLZT, while voltage thickness in almost same as ceramics. These differences are due to characteristics configuration electrode. A simple model is used for phenomenological explanation effect film.
ABSTRACT We have isolated the genomic and cDNA clones encoding EG III (a low-molecular-mass endo-β-1,4-glucanase) gene from Trichoderma reesei QM9414. The nucleotide sequence of fragment was verified to contain a 702-bp open reading frame that encodes 234-amino-acid propeptide. deduced protein has significant homologies with family H endo-β-1,4-glucanases. 16-amino-acid N-terminal shown function as leader peptide for possible secretion. Northern blot analysis showed transcript, length about...
Daily transpiration before and after thinning was measured on six individual trees in a 31-year-old Chamaecyparis obtusa Endl. stand by the heat pulse method. After thinning, daily of tree at given level solar radiation increased, difference between increased with radiation. The increase related to high rate crown caused greater canopy exposure and, subsequently, foliage biomass per tree. Stand calculated basis two parameters, maximum vapor saturation deficit air. During growing season...
Penicillium chrysogenum was immobilized in polyacrylamide gel prepared from 5% acrylamide monomers (85% and 15% N,N'-methylene bisacrylamide). Penicillin produced glucose by the mycelium 17% of that washed mycelium. However, activity penicillin production decreased with repeated use. On other hand, increased initially gradually The rate oxygen uptake about 30% required for penicillin.
Abstract Whole cells of Bacillus sp., a bacitracin‐producing bacteria, were immobilized in polyacrylamide gel. The continuous production bacitracin by an whole‐cell‐containing air‐bubbled reactor was examined with 0.5% peptone solution. productivity (28 units/ml/hr) obtained this system higher than that batch system. effluent concentration increased increasing aeration rate and reached steady‐state maximum above the 3.0 liter/min. A high retained for at least eight days when gel washed...
One of the limiting factors for application Trichoderma reesei to degrade cellulosic biomass is its low β-glucosidase activity, required convert cellobiose glucose. The egl3 and xyn3 promoters were used express 1 gene bgl1 through homologous recombination improve cellulose degradation ability T. reesei. recombinant strains expressing (BGLI) under control either or promoter had 4.0 7.5 fold higher activity than native strain, which compares well finding that in wild-type PC-3-7, levels mRNA...
Although lactose is a preferred cellulase inducer in the industrial production of by Trichoderma reesei, mechanism induction not fully understood. Because sugar transporters might be involved at an early step oligosaccharides, we sought permeases associated with lactose. Two such MFS T. reesei hyper-cellulolytic PC-3-7 strain, producer developed Japan, were identified screening for permeases. Disruption genes encoding these two resulted decreased uptake and delayed growth culture. Further,...
The filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina) produces increased cellulase expression when grown on cellulose or its derivatives as a sole carbon source. It has been believed that β-glucosidases T. not only metabolize cellobiose but also contribute in the production inducers gene by their transglycosylation activity. hyper-producing mutant PC-3-7 developed Japan enhanced ability is used inducer. comparative genomics analysis and parent revealed single-nucleotide...
Chitosan-degrading activities induced by glucosamine (GlcN) or N-acetylglucosamine (GlcNAc) were found in a culture filtrate of Trichoderma reesei PC-3-7. One the chitosan-degrading enzymes was purified to homogeneity precipitation with ammonium sulfate followed anion-exchange and hydrophobic-interaction chromatographies. The enzyme monomeric, its molecular mass 93 kDa. optimum pH temperature 4.0 50 degrees C, respectively. activity stable range 6.0 9.0 at below C. Reaction product analysis...
Photovoltaic (Pb,La)(Zr,Ti)O3 films in a layered structure of different crystallographic orientations are fabricated by an optimized metalorganic deposition method. Such (001) orientation exhibit photovoltaic electrical power approximately 20 times higher than that random films. The anisotropic optical properties the oriented films, including dark conductivity, photoconductivity, and tensor surfaces, obtained quantitatively. These results show output current highly improved to be equal those...
The ascomycete Trichoderma reesei is known as one of the most prolific producers plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety industrial applications, little about mechanical basis these improvements. A genomic sequence comparison and wild-type was undertaken provide new insights in this regard. We identified number single-nucleotide polymorphisms (SNPs) after sequencing genome hyper-cellulolytic...