A5030658601- Plant Physiology and Cultivation Studies
- Plant Pathogens and Fungal Diseases
- Horticultural and Viticultural Research
- Viral gastroenteritis research and epidemiology
- Garlic and Onion Studies
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Probiotics and Fermented Foods
- Viral Infections and Immunology Research
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Fungal Plant Pathogen Control
- Virus-based gene therapy research
- Phytochemical Studies and Bioactivities
- Gut microbiota and health
- Food Quality and Safety Studies
- Microbial Metabolites in Food Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolism and Applications
- Respiratory viral infections research
- Agriculture, Soil, Plant Science
- Genomics and Phylogenetic Studies
- Phytochemistry and Bioactive Compounds
- Plant and Fungal Interactions Research
Keio University
2013-2025
University of Tsukuba
2024-2025
Kanagawa Institute of Technology
2025
Tsukuba Medical Center Hospital
2025
Juntendo University
2025
Saga University
2016-2024
Tokyo University of Agriculture and Technology
2024
National Agriculture and Food Research Organization
2024
Research Center for Agricultural Information Technology
2024
Kagoshima University
2021
Probiotics are live microorganisms that potentially confer beneficial outcomes to host by modulating gut microbiota in the intestine. The aim of this study was comprehensively investigate effects probiotics on human intestinal using 454 pyrosequencing bacterial 16S ribosomal RNA genes with an improved quantitative accuracy for evaluation composition. We obtained 158 faecal samples from 18 healthy adult Japanese who were subjected intervention 6 commercially available containing either...
Lactobacillus reuteri is a heterofermentative lactic acid bacterium that naturally inhabits the gut of humans and other animals. The probiotic effects L. have been proposed to be largely associated with production broad-spectrum antimicrobial compound reuterin during anaerobic metabolism glycerol. We determined complete genome sequences reuterin-producing JCM 1112T its closely related species fermentum IFO 3956. Both are in same phylogenetic group within genus Lactobacillus. Comparative...
ABSTRACT Noroviruses (NVs) cause many cases of oyster- or clam-associated gastroenteritis in various countries. We collected 191 samples from Japanese oysters intended for raw consumption that had been harvested the sea two different areas between December 2001 and February 2002. To detect, quantitate, phylogenetically analyze NV genome purified concentrates stomachs digestive diverticula these oysters, we amplified capsid gene by reverse transcription-PCR. Phylogenetic analysis was...
Strains HM2-1 and HM2-2(T) were isolated from the faeces of a healthy infant characterized by determining their phenotypic biochemical features phylogenetic positions based on partial 16S rRNA gene sequence analysis. They Gram-positive, obligately anaerobic, non-spore-forming, non-gas-producing, catalase-negative non-motile rods. did not grow at 15 or 45 °C in anaerobic bacterial culture medium, DNA G+C content was range 56-59 mol%. In enzyme activity tests, strains positive for...
Ulcerative colitis (UC) is significantly linked with gut microbiota, which essential for maintaining health. Their metabolites mitigate inflammation and bolster barrier function. Among these metabolites, we focused on vitamin B3, has been reported to improve the pathogenesis of UC in mice. This study aimed compare fecal B3 microbiota between non-UC patients. We assessed 71 patients (UC group) 72 (non-UC matched by sex age 10-year intervals. Fecal samples were collected analyzed using...
Norovirus (NoV) is a major etiological agent of acute gastroenteritis outbreaks worldwide. A total 314 fecal specimens collected from patients 39 NoV in Hiroshima Prefecture, Japan, between December 2001 and April 2006 were tested for the occurrence recombinant NoVs. Sixteen genotypes (GI/1, GI/2, GI/4, GI/7, GI/8, GI/11, GI/14, GII/2, GII/3, GII/4, GII/5, GII/6, GII/8, GII/12, GII/14, GII/untypeable) detected based on capsid sequences GII/4 was predominant recently. Twelve strains 11...
The genetic variation of loquat (Eriobotrya japonica (Thunb.) Lindl.) was characterized by SSR markers developed from apple and pear, using 94 accessions in Japan. Fourteen the 24 derived could successfully produce amplified bands loquat, whereas 10 pear generate bands. Nine were chosen for evaluation diversity among accessions, including 61 cultivars Japan other countries 33 natively grown collected around A phenogram constructed unweighted pair-group method with arithmetic averages based...
Loquat canker ( Pseudomonas syringae pv. eriobotryae ) is a serious disease of loquat Eriobotrya japonica ), and no commercial cultivar in Japan resistant to all strains the disease. Shiromogi, which was selected from progeny seedlings ‘Mogi’, Group C has good fruit characteristics. This study conducted determine inheritance resistance ‘Shiromogi’. Seedlings produced crosses between two 13 susceptible genotypes were classified as either (R) or (S) based on appearance black–brown cankers ≈2...
Three new ferulic acid esters of glucosylglucose, 1-O-(E)-feruloyl-β-d-glucopyranosyl (1-2)-[β- d-glucopyranosyl (1-6)]-β-d-glucopyranose (allimacronoid A, 1), 1-O-(E)-feruloyl-{β-d-glucopyranosyl (1-4)-[β-d-glucopyranosyl (1-2)]}-[β- B, 2), and (1-6)-[β-d-glucopyranosyl C, 3) were isolated together with tuberonoid A (4), from the leaves Allium macrostemon Bunge. The chemical structures elucidated based on analyses spectroscopic data.
2009 年4 月にメキシコ，アメリカで確認された豚由来の新型インフルエンザの迅速診断および季節性A 型インフルエンザウイルスの判別を目的にReverse Transcription（RT）-LAMP 法によるインフルエンザウイルスの検出法を検討した．新型インフルエンザウイルスA（H1N1）および季節性インフルエンザウイルスA ソ連型（H1N1），A 香港型（H3N2）の各HA 遺伝子の遺伝子配列を基にプライマーを設計し，新型，A ソ連型，A 香港型の検出系を構築した．63℃で40 分間のRT-LAMP 反応では，各亜型およびB 型インフルエンザウイルス，C 型インフルエンザウイルス，アデノウイルス，RS ウイルス，メタニューモウイルス，パラインフルエンザウイルスおよびライノウイルスとの交差反応は認められず，新型，A 香港型をそれぞれ特異的に検出できることが確認された．また，RT-LAMP 反応液に加えたエリオクロムブラックT...
Loquat canker, caused by Pseudomonas syringae pv. eriobotryae , is a serious disease of loquat [ Eriobotrya japonica (Thunb.) Lindl.] in some countries such as Japan. Therefore, improved canker resistance an important objective for breeding. The to Group C descendants ‘Shiromogi’ was expressed only homozygotes with recessive gene at single locus, which designated pse-c/pse-c . ‘Champagne’, distantly related ‘Shiromogi’, another cultivar C. inheritance this progenies crosses between...
Allium macrostemon Bunge, commonly referred to as "no-biru" in Japan, is a widespread wild onion species found across the country. Despite being deeply entwined ancient Japanese culture, it remains an underutilized crop Japan. Determining origins of its domestic populations and understanding their genetic composition crucial highlighting plant's historical significance This study aims bridge this knowledge gap by examining diversity 47 A. samples from various regions Japan using RAD-Seq. Our...
Two new ferulic acid esters of oligo-glucose, 1-Ο-(E)-feruloyl-β--D-gentiobioside (1) and 1-Ο-(E)feruloyl-{β-D-glucopyranosy (1->6)-[β-D-glucopyranosyl (I--2)]}-β-D-glucopyranoside.(allimacronoid D, 2) were isolated together with 1-Ο-(E)-feruloyl-β-D-glucopyranoside (3) trans-ferulic (4) from the leaves Allium macrostemon Bunge. The chemical structures elucidated based on analyses spectroscopic data.
Multiplex reverse transcription (RT)-polymerase chain reaction (PCR)-based assays involving fluorescent dye-labeled primers were modified to detect 10 types of gastroenteritis viruses by adding two further a previously developed assay. Then, these applied clinical samples, which collected between January 2006 and December 2013. All effectively detected in the multiplex RT-PCR-based assays. In addition, various viral parameters, such as detection rates age distributions each type, examined....
ビワのSハプロタイプをシークエンサーを用いて，効率的に推定する方法について検討を行った．その結果，その有用性を確認するとともに，10の異なるバンドサイズを確認することができた．そのうち5つについては，既報のビワS-RNase遺伝子と塩基配列および推定アミノ酸配列において100％の相同性を示したが，残り5つは，既報のビワS-RNase遺伝子と異なる配列を示す一方で，推定アミノ酸配列において，ビワと同じバラ科ナシ亜科に属するリンゴおよびセイヨウナシのS-RNaseと高い相同性を示した．また，いずれの配列についても，システインおよびヒスチジン残基が保存されており，今回新たに確認された5つのバンドについては，新規のビワS-RNase遺伝子である可能性が高いと考えられた．これらの結果に基づき，国内外145品種・系統のビワについてSハプロタイプの推定を行ったところ，25タイプに分けることができ，‘茂木’，‘田中’および‘長崎早生’など日本における主要な栽培品種は自家和合性であることが遺伝子レベルでも確認された．また，41品種・系統は自家不和合性であると推定された．
Biologically active compounds in leaves of Lycium chinense (Lycii Folium) were examined to develop the effective use this plant as a health food. The methanol extract Lycii Folium exhibited antimicrobial activities, and one was identified lyciumoside I (1) which is characteristic component chinense. Furthermore, inhibited growth Helicobacter pylori. against H. pylori also 1. Quantitative analyses revealed that 1 extracted into Kuko tea high concentration. hydrolysis products assayed for...