A5086669444- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- COVID-19 diagnosis using AI
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Immunodeficiency and Autoimmune Disorders
- COVID-19 epidemiological studies
- Genomics and Phylogenetic Studies
- Animal Virus Infections Studies
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Poxvirus research and outbreaks
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Bacillus and Francisella bacterial research
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Genital Health and Disease
- Infectious Diseases and Tuberculosis
- Ocular Infections and Treatments
Hospital Universitario Nuestra Señora de Candelaria
2017-2024
Organización Nacional de Trasplantes
2023
TIE Kinetix (Germany)
2023
Hospital Universitario 12 De Octubre
2023
Hospital Universitario de Canarias
2017
The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription quantitative PCR (RT-qPCR). Based the urgent need for high-throughput screening, we tested performance of three alternative, simple affordable protocols to rapidly detect SARS-CoV-2, bypassing long tedious extraction step reducing time viral detection.We evaluated methods based direct nasopharyngeal swab transmission medium (VTM) heating before RT-qPCR: a) without...
Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory neurological disease. We collected samples from several European countries during the 2018 outbreak determined 53 near full-length genome ('whole genome') sequences. These sequences were combined with 718 whole 1,987 VP1-gene publicly available In 2018, circulating strains clustered into multiple subgroups B3 A2 subclades, different phylogenetic origins. Clusters subclade emerged primarily US...
ABSTRACTThe emergence of the Omicron SARS-CoV-2 variant concern has changed COVID-19 scenario as this is characterized by high transmissibility and immune evasion ability. To evaluate impact on Canary Islands (Spain) population, we determined reinfection rates disease severity associated with sublineages previously circulating variants concern. We performed a retrospective observational study 21,745 viral genomes collected from December 2020 to July 2022 in (Spain). compared between lineages...
ObjectivesThe ongoing COVID-19 pandemic continues to impose demands on diagnostic screening. In anticipation that the recurrence of outbreaks and measures for lifting lockdown worldwide may cause supply chain issues over coming months, this study assessed sensitivity a number one-step retrotranscription quantitative polymerase reaction (RT-qPCR) solutions detect SARS-CoV-2.MethodsSix different RT-qPCR alternatives were evaluated SARS-CoV-2/COVID-19 diagnosis based standard RNA extractions....
Limited testing capacity has characterized the ongoing coronavirus disease 2019 (COVID-19) pandemic in Spain, hampering timely control of outbreaks and opportunities to reduce escalation community transmission. This study investigated potential use sample pooling, followed by one-step retrotranscription real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) increase for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).Various pool sizes (five, 10 15...
Respiratory syncytial virus (RSV) is a leading cause of acute respiratory illness, especially in children, immunocompromised patients, and the elderly. Genomic surveillance RSV enables detecting monitoring circulating lineages emergence amino acid substitutions affecting transmission, severity, treatment, including resistance to monoclonal antibodies (mAb). Most available genomic protocols target specific regions viral genome, such as G F genes, or are designed for particular sequencing...
Abstract The current reference for COVID-19 diagnosis is based on the detection of SARS-CoV-2 RNA extracts using one-step retrotranscription and quantitative PCR (RT-qPCR). Based urgent need high-throughput screening, we tested performance three alternative, simple affordable protocols to rapidly detect SARS-CoV-2, overcoming long tedious extraction step. Although with an average increase 6.1 (± 1.6) cycles compared standard tests extracts, show that RT-qPCR yielded consistent results in...
Mycoplasma genitalium (MG) is a recognized sexually transmitted pathogen. Increasing resistance to main lines of treatment (macrolides and quinolones) justifies genetic study mutations improve cure rates.A total 8,508 samples from April 2018 July 2022 were processed using AllplexTM STI Essential Assay. In MG positive cases 23S rRNA V domain, gyrA parC genes studied. Mutations detected checked assess their clinical significance medical records reviewed obtain demographic...
Vancomycin-resistant enterococci (VRE) infections and outbreaks are still infrequent in Spain. A six-month outbreak, which took place a haematology ward, its control management described this study. total of 22 patients were colonised two bloodstream occurred during period. Even though there waves new patients, multidisciplinary approach, quick interventions enhanced infection policies required order to outbreak.
Abstract Objective The ongoing COVID-19 pandemic continues imposing a demand for diagnostic screening. In anticipation that the recurrence of outbreaks and measures lifting lockdown worldwide may cause supply chain issues over coming months, we assessed sensitivity number one-step retrotranscription quantitative PCR (RT-qPCR) solutions to detect SARS-CoV-2. Methods We evaluated six different RT-qPCR alternatives SARS-CoV-2/COVID-19 diagnosis based on standard RNA extractions. That best was...
Abstract Objectives Limited testing capacity has characterized the ongoing COVID-19 pandemic in Spain, hampering a timely control of outbreaks and possibilities to reduce escalation community transmissions. Here we investigated potential using pooling samples followed by one-step retrotranscription quantitative PCR (RT-qPCR) increase SARS-CoV-2 capacity. Methods We first evaluated different sample (1:5, 1:10 1:15) prior RNA extractions standard RT-qPCR for SARS-CoV-2/COVID-19 diagnosis. The...
Several variants of concern (VOCs) explain most the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemic waves in Europe. We aimed to dissect spread SARS-CoV-2 VOCs Canary Islands (Spain) between December 2020 and September 2021 at a micro-geographical level. sequenced viral genome 8,224 samples collected archipelago. observed that Alpha (B.1.1.7) Delta (B.1.617.2 sublineages) were ubiquitously present islands, while Beta (B.1.351) Gamma (P.1/P.1.1) had heterogeneous...
On July 23, 2022, monkeypox disease (mpox) was declared a Public Emergency of International Concern (PHEIC) by the World Health Organization (WHO) due to multicountry outbreak. In Europe, several cases mpox virus (MPXV) infection related this outbreak were detected in Canary Islands (Spain). Here we describe combination viral DNA sequencing and bioinformatic approaches, including methods for de novo genome assembly short- long-read technologies, used reconstruct first MPXV isolated on 31st...
Background Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory neurological disease. Methods We collected samples from several European countries during the 2018 out-break determined 53 near full-length genome (‘whole genome’) sequences. These sequences were combined with 718 whole 1,987 VP1-gene publicly available Findings In 2018, circulating strains clustered into multiple subgroups B3 A2 subclades, different phylogenetic origins. Clusters...