A5024787078- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- Chromosomal and Genetic Variations
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cytokine Signaling Pathways and Interactions
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Parvovirus B19 Infection Studies
- HIV/AIDS drug development and treatment
- CAR-T cell therapy research
- Flood Risk Assessment and Management
- Chemical Synthesis and Analysis
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Lipid Membrane Structure and Behavior
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
Martin Luther University Halle-Wittenberg
2012-2025
University of East Anglia
2012-2024
Vivantes Klinikum
2023
Thomas Jefferson University
2021
Institute of Immunology
2020
Luther University
2009-2020
University of Reading
2002-2019
European Centre for Medium-Range Weather Forecasts
2015-2019
Norwich Research Park
2016-2019
Universitätsklinikum Erlangen
2006-2019
The skill of a forecast can be assessed by comparing the relative proximity both and benchmark to observations. Example benchmarks include climatology or naïve forecast. Hydrological ensemble prediction systems (HEPS) are currently transforming hydrological forecasting environment but in this new field there is little information guide researchers operational forecasters on how best used evaluate their probabilistic forecasts. In study, it identified that calculated vary depending selected...
The human endogenous retrovirus K10 (HERV-K10) has been identified in the genome by its homology to retroviruses of other vertebrates (M. Ono, T. Yasunaga, Miyata, and H. Ushikubo, J. Virol. 60:589-598, 1986). Using PCR amplification, DNA cloning, sequencing, procaryotic expression, we were able demonstrate that HERV-K10 encodes a 73-kDa protein which was processed HERV-K10-encoded protease yield proteins p22/p26, p30, p15/16. Analysis teratocarcinoma cell line Tera 1 or tumor tissues...
Mouse 6Ckine/SLC (secondary lymphoid tissue chemokine) is a chemotactic factor for dendritic cells, T and NK cells in vitro. In addition, mouse interacts with the chemokine receptor CXCR3, as do several chemokines antiangiogenic properties. These dual properties of were tested induction an antitumor response by transducing C26 colon carcinoma tumor cell line cDNA encoding 6Ckine/SLC. The C26-6CK-transduced showed reduced tumorigenicity immunocompetent or nude mice. Part this effect was...
In several vertebrate species, Borna disease virus (BDV), the prototype of a new group animal viruses, causes central nervous system accompanied by diverse behavioral abnormalities. Seroepidemiological data indicate that BDV may contribute to pathophysiology certain human mental disorders. This hypothesis is further supported detection both antigens and RNA in peripheral blood mononuclear cells (PBMCs) patients with psychiatric disorders isolation from such PBMCs. Here we describe...
Abstract The chemokine receptor CXCR3 is expressed on the surface of both resting and activated T lymphocytes. We describe in this study endocytosis using lymphocytes transfectants. Chemokine-induced down-regulation occurred a rapid, dose-dependent manner, with CXCL11 most potent efficacious ligand. Endocytosis was mediated part by arrestins, but appeared to occur independently clathrin caveolae. In contrast other receptors, which are largely recycled cell within an hour, replenishment over...
Abstract The chemokine CXCL4/platelet factor-4 is released by activated platelets in micromolar concentrations and a chemoattractant for leukocytes via an unidentified receptor. Recently, variant of the human receptor CXCR3 (CXCR3-B) was described, which transduced apoptotic but not chemotactic signals microvascular endothelial cells following exposure to high CXCL4. Here, we show that CXCL4 can induce intracellular calcium release migration T lymphocytes. CXCL4-induced chemotaxis...
Downregulation of HLA class I expression may contribute to a poor prognosis in cancer patients. There is limited information about epigenetic and oncogenic regulation I, multiple mechanisms be involved. In the current study, we examined relationship between HER2-signaling pathway (MAPK PI3K-Akt) Ag-processing machinery (APM) components. A panel gastric esophageal cell lines was treated with wortmannin as an Akt-signal inhibitor; MAPK signal inhibitor PD98059; lapatinib, which inhibits both...
Abstract Activated platelets release micromolar concentrations of the chemokine CXCL4/Platelet Factor-4. Deposition CXCL4 onto vascular endothelium is involved in atherosclerosis, facilitating monocyte arrest and recruitment by an as yet, unidentified receptor. Here, we demonstrate that drives chemotaxis monocytic cell line THP-1. Migration intracellular calcium responses induced were pertussis toxin-sensitive, implicating a GPCR signal transduction. Cell treatment with chondroitinase ABC...
Silicon nanoparticles (SiNPs) hold prominent interest in various aspects of biomedical applications. For this purpose, surface functionalization the NPs is essential to stabilize them, target them specific disease area, and allow selectively bind cells or bio-molecules present on cells. However, no such has been explored with Si nanoparticles. Carbohydrates play a critical role cell recognition. Here, we report first synthesis silicon functionalized carbohydrates. In study, stable brightly...
Pharyngitis caused by Streptococcus pyogenes is one of the most common bacterial infections in humans and also a starting point for invasive S. infection. Here, we describe that tonsil fluid from patients with streptococcal pharyngitis contains high amounts interferon (IFN)-dependent CXC chemokine known as monokine induced IFN- gamma (MIG)/CXCL9. Also vitro, inflamed pharyngeal epithelium produced large MIG/CXCL9 presence bacteria. The chemokines MIG/CXCL9, IFN-inducible protein-10/CXCL10, T...
A rapid synthetic route to a nontoxic fluorescently labeled water-soluble calixarene has been developed. Investigation of the cellular uptake calixarene, via confocal microscopy, through coincubation with inhibitors demonstrates that is not common clathrin coated pits or caveolae (lipid raft) endocytic pathways and derivative localizes within cytoplasm does enter nucleus. The study power fluorescent labeling for investigation interactions between calixarenes biological systems potential...
We have recently identified Np9 as a novel nuclear protein produced by the human endogenous retrovirus K and were able to document exclusive presence of np9 transcript in tumors transformed cells. With aim studying whether has role tumorigenesis, systematic search for interacting proteins was performed. Here, we identify RING-type E3 ubiquitin ligase LNX (ligand Numb X) an Np9-interacting partner. furthermore show that interaction involves N- C-terminal domains both can affect subcellular...
More than 2000 human endogenous retrovirus (HERV) sequences are present in the genome, yet only a few intact and able to produce proteins. The normal functions of these, if any, unknown, but some HERV proteins have been implicated cancers, particular germ-cell cancers. For instance, it has documented that (i) patients with tumours frequently antibodies against proteins; (ii) transgenic mice expressing HERV-K (HML-2) rec prone testicular carcinoma situ; (iii) Rec can bind suppress guardian...
Abstract Cancer immunotherapy can result in durable tumor regressions some patients. However, patients who initially respond often experience progression. Here, we report mechanistic evidence of tumoral immune escape an exemplary clinical case: a patient with metastatic melanoma developed disease recurrence following initial, unequivocal radiologic complete regression after T-cell–based immunotherapy. Functional cytotoxic T-cell responses, including responses to one mutant neoantigen, were...
Tumor escape is often associated with abnormalities in the surface expression of human leukocyte antigen class I (HLA-I) antigens thereby limiting CD8+ cytotoxic T cell responses. This impaired HLA-I can be mediated by deficient components processing and presentation machinery (APM) due to epigenetic, transcriptional and/or post-transcriptional processes. Since a discordant mRNA protein pattern APM including peptide transporter 1 (TAP1) has been frequently described tumors distinct origin,...
Novel amino-functionalised multicalixarenes have been synthesised which show low cellular toxicity, effective DNA binding and, when featuring aliphatic amines, are efficient gene transfection agents.