A5100388691- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Clostridium difficile and Clostridium perfringens research
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Microbial Metabolic Engineering and Bioproduction
- Anaerobic Digestion and Biogas Production
- Mitochondrial Function and Pathology
- Cytomegalovirus and herpesvirus research
- Ocular Disorders and Treatments
- Gut microbiota and health
- Plant Diversity and Evolution
- Cleft Lip and Palate Research
- Genetic diversity and population structure
- Craniofacial Disorders and Treatments
- Microbial Fuel Cells and Bioremediation
- DNA Repair Mechanisms
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Hepatitis B Virus Studies
- Antimicrobial Peptides and Activities
- Phytochemical Studies and Bioactivities
- Hepatitis Viruses Studies and Epidemiology
University of Science and Technology of China
2023-2024
Center for Excellence in Molecular Plant Sciences
2017-2020
Chinese Academy of Sciences
2013-2020
University of Chinese Academy of Sciences
2013-2020
University of Nebraska–Lincoln
2014-2017
Fujian Institute of Research on the Structure of Matter
2017
Shanghai Institutes for Biological Sciences
2017
State Key Laboratory of Medicinal Chemical Biology
2016
Nankai University
2016
Kunming Medical University
2014
The oxidation of l -proline to glutamate in Gram-negative bacteria is catalyzed by the proline utilization A (PutA) flavoenzyme, which contains dehydrogenase (PRODH) and Δ 1 -pyrroline-5-carboxylate (P5C) domains a single polypeptide. Previous studies have suggested that aside from providing energy, metabolism influences oxidative stress resistance different organisms. To explore this potential role mechanism, we characterized wild-type putA mutant strains Escherichia coli . Initial assays...
Catabolite control protein A (CcpA) is the master regulator in Gram-positive bacteria that mediates carbon catabolite repression (CCR) and activation (CCA), two fundamental regulatory mechanisms enable competitive advantages catabolism. It generally regarded CcpA exerts its role by binding to a typical 14- 16-nucleotide (nt) consensus site called response element (cre) within target regions. However, here we report previously unknown noncanonical flexible architecture of CcpA-binding...
ABSTRACT The master regulator CcpA (catabolite control protein A) manages a large and complex regulatory network that is essential for cellular physiology metabolism in Gram-positive bacteria. Although can affect the expression of target genes by binding to cis -acting catabolite-responsive element ( cre ), whether how regulated remain poorly explored. Here, we report novel dual- motif employed Clostridium acetobutylicum , typical solventogenic species, autoregulation. Two sites are involved...
Background The origin of extraordinarily rich biodiversity in tropical forests is often attributed to evolution under stable climatic conditions over a long period or fluctuations during the recent Quaternary period. Here, we test these two hypotheses using Dracaena cambodiana, plant species distributed paleotropical forests. Methods We analyzed nucleotide sequence data chloroplast DNA (cpDNA: atpB-rbcL and trnD-trnT) regions genotype six nuclear microsatellites from 15 populations (140 363...
Abstract WhiB1 is a monomeric iron–sulfur cluster-containing transcription factor in the WhiB-like family that widely distributed actinobacteria including notoriously persistent pathogen Mycobacterium tuberculosis (M. tuberculosis). plays multiple roles regulating cell growth and responding to nitric oxide stress M. tuberculosis, but its underlying mechanism unclear. Here we report 1.85 Å-resolution crystal structure of [4Fe–4S] cluster-bound (holo-) complex with C-terminal domain σ70-family...
Protein lysine acetylation, a prevalent posttranslational modification, regulates numerous crucial biological processes in cells. Nevertheless, how acetylation interacts with other types of regulation to coordinate metabolism remains largely unknown owing the complexity process. Here, using representative gas-fermenting bacterium, Clostridium ljungdahlii, we revealed novel regulatory mechanism that employs both and transcriptional systems interactively control CO2 fixation, key process for...
DExD/H box RNA helicases play essential roles in various biological processes prokaryotes and eukaryotes. By screening Pseudomonas aeruginosa strains with mutations helicase genes, we identified that deaD was required for bacterial cytotoxicity virulence a mouse acute pneumonia model. Compared to wild-type strain its complementation strain, the mutant induced less production of proinflammatory cytokines, neutrophil infiltration lung damage during infection. We further found activity DeaD...
SUMMARY DksA and ppGpp are the central players in Escherichia coli stringent response mediate a complete reprogramming of transcriptome from one optimized for rapid growth to adapted survival during nutrient limitation. A major component is reduction ribosome synthesis, which accomplished by synergistic action bound RNA polymerase (RNAP) inhibiting transcription rRNAs. Here, we report X-ray crystal structures E. RNAP holoenzyme complex with alone ppGpp. The show that accesses template strand...
Proline utilization A from Escherichia coli (EcPutA) is a multifunctional flavoenzyme that oxidizes proline to glutamate through dehydrogenase (PRODH) and Δ1-pyrroline-5-carboxylate (P5CDH) activities, while also switching roles as DNA-bound transcriptional repressor membrane-bound catabolic enzyme. This phenomenon, termed functional switching, occurs redox-mediated mechanism in which flavin reduction triggers conformational change increases EcPutA membrane binding affinity. Structural...
The uses of sRNAs as new resources for functional studies and strain modifications are promising strategies in microorganisms. However, these crucial regulatory molecules have hardly been explored industrially important solventogenic clostridia. Here, we identified sr8384 a novel determinant sRNA controlling the cell growth Clostridium acetobutylicum . Based on detailed analysis, further reveal pleiotropic function its multiple direct indirect targets, which represents valuable source...
Objective To investigate the approach and clinical efficacy of second repair surgery according to nose mouth contour design unilateral cleft lip.Methods Based on characteristics anatomy,mechanics deformity lip,we designed a surgical incision nasolabial lines with reference horizontal central vertical contour.After careful investigation classic procedures practice,unilateral lip was repaired by overall stage Ⅱ procedures.Results Application this method satisfactory had been...
Abstract Methods for engineering protease specificity and selectivity toward target substrates of therapeutic or industrial relevance are wide interest. Herein, we report a bacterial system the simultaneous detection selection activity on positive negative substrates. The system, based Proteolysis‐triggered bActerial SuiCide Antibiotic Resistance (PASCAR) in Escherichia coli , exploits β‐lactamase human gasdermin D (GS) proteolysis‐responsive selections, respectively. applicability was...
ABSTRACT Small noncoding RNAs (sncRNAs) are crucial regulatory molecules in organisms and well known not only for their roles the control of diverse essential biological processes but also value genetic modification. However, to date, gram-positive anaerobic solventogenic clostridia (which a group important industrial bacteria with exceptional substrate product diversity), sncRNAs remain minimally explored, leading lack detailed understanding regarding these use as targets improvement. Here,...