A5074869721- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Metabolism and Genetic Disorders
- Advanced Proteomics Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Traditional Chinese Medicine Studies
- Lipid metabolism and biosynthesis
- Healthcare and Venom Research
- Analytical Chemistry and Chromatography
- Sphingolipid Metabolism and Signaling
- Cancer, Hypoxia, and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Peroxisome Proliferator-Activated Receptors
- Lipid Membrane Structure and Behavior
Tsinghua University
2017-2023
Ablation of Slc22a14 causes male infertility in mice, but the underlying mechanisms remain unknown. Here, we show that SLC22A14 is a riboflavin transporter localized at inner mitochondrial membrane spermatozoa mid-piece and by genetic, biochemical, multi-omic, nutritional evidence transport deficiency suppresses oxidative phosphorylation reprograms energy metabolism disrupting flavoenzyme functions. Specifically, find fatty acid β-oxidation (FAO) defective with significantly reduced levels...
Understanding the tissue distribution of phospholipids and glycerolipids in animal models enables promoting pharmacokinetic study drugs related PK predictions. The measurement lipid compositions models, usually mice rats, without a standardized approach hindered accuracy PBPK investigation. In this work, high resolution mass spectrometry was applied to profile 12 organs/tissues rats. Using method, not only amounts each organ/tissue but also fatty acid were acquired. order explore...
Objective: To explore the pharmacological basis and mechanism of Buxue Yimu pills (BYP) in treatment anaemia women from perspective metabolomics network analysis. Materials Methods: Forty-six reproductive age with haemoglobin 70-110 g/L were recruited. Blood samples collected before after 4 weeks oral BYP to assess changes haemoglobin, coagulation function, iron metabolism indices. An integrated analysis (liquid chromatography mass spectrometry) was performed identify potential...
Ablation of Slc22a14 causes male infertility in mice, but the underlying mechanisms remain unknown. Here, we discovered that SLC22A14 is a riboflavin transporter localized at mitochondrial inner membranes spermatozoa midpiece, and showed via genetic, biochemical, multi-omic nutritional evidence transport deficiency suppresses oxidative phosphorylation reprograms energy metabolism by disrupting flavoenzyme functions. Specifically, found fatty acid β-oxidation (FAO) defective with...
Isotope tracing assisted metabolic analysis is becoming a unique tool to understand regulation in cell biology and biomedical research. Targeted mass spectrometry based on selected reaction monitoring (SRM) has been widely applied isotope experiment with the advantages of high sensitivity broad linearity. However, its application for new pathway discovery largely restrained by molecular coverage. To overcome this limitation, we describe strategy called pseudo-targeted profiling isotopic...