A5053111091- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- Temporomandibular Joint Disorders
- Ginseng Biological Effects and Applications
- Immune cells in cancer
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Histone Deacetylase Inhibitors Research
- Metabolism and Genetic Disorders
- interferon and immune responses
- Epigenetics and DNA Methylation
- Amino Acid Enzymes and Metabolism
- Traditional Chinese Medicine Analysis
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Studies on Chitinases and Chitosanases
- Silicone and Siloxane Chemistry
- Cancer Immunotherapy and Biomarkers
Tsinghua University
2017-2025
The Second Affiliated Hospital of Bengbu Medical College
2024
Xian Yang Central Hospital
2024
Jiangxi University of Traditional Chinese Medicine
2024
First Affiliated Hospital of Bengbu Medical College
2022
Harbin Institute of Technology
2022
Beijing University of Technology
2021
Institute of Botany
2014-2020
Chinese Academy of Sciences
2014-2020
Institute of Hematology & Blood Diseases Hospital
2015-2017
ZNF750 controls epithelial homeostasis by regulating epidermal-differentiation genes, a role underscored its pathogenic mutations in esophageal squamous cell cancers (SCCs). However, the precise of SCC biology remains unclear. In this study, we report that is exclusively deleted, mutated and underexpressed human SCCs, low expression associated with poor survival. Restoration wildtype, but not mutant protein uniquely inhibited malignant phenotypes cells both vitro vivo. Notably, promoted long...
Histone ubiquitination affects the structure and function of nucleosomes through tightly regulated dynamic reversible processes. The efficient preparation ubiquitinated histones their analogs is important for biochemical biophysical studies on histone ubiquitination. Here, we report CAACU (cysteine-aminoethylation assisted chemical ubiquitination) strategy synthesis analogs. key step in installation an N-alkylated 2-bromoethylamine derivative into a recombinant cysteine aminoethylation,...
Abstract NKCC and KCC transporters mediate coupled transport of Na + +K +Cl − K across the plasma membrane, thus regulating cell Cl concentration volume playing critical roles in transepithelial salt water neuronal excitability. The function these has been intensively studied, but a mechanistic understanding awaited structural studies transporters. Here, we present cryo-electron microscopy (cryo-EM) structures two cation-chloride cotransporters human NKCC1 (SLC12A2) mouse KCC2 (SLC12A5),...
Ablation of Slc22a14 causes male infertility in mice, but the underlying mechanisms remain unknown. Here, we show that SLC22A14 is a riboflavin transporter localized at inner mitochondrial membrane spermatozoa mid-piece and by genetic, biochemical, multi-omic, nutritional evidence transport deficiency suppresses oxidative phosphorylation reprograms energy metabolism disrupting flavoenzyme functions. Specifically, find fatty acid β-oxidation (FAO) defective with significantly reduced levels...
Activity-based E2 conjugating enzyme (E2)-ubiquitin (Ub) probes have recently emerged as effective tools for studying the molecular mechanism of E3 ligase (E3)-catalyzed ubiquitination. However, preparation existing activity-based E2-Ub depends on recombination technology and bioconjugation chemistry, limiting their structural diversity. Herein we describe an expedient total chemical synthesis variant through a hydrazide-based native ligation, which enabled construction structurally new...
Flavonoids are widely distributed in plants as secondary metabolites and have various biological benefits such anti-tumor, anti-oxidant, anti-inflammatory anti-aging. We previously reported that 4,4'-dimethoxychalcone (DMC) suppressed cancer cell proliferation by aggravating oxidative stress inducing G2/M cycle arrest. In the present study, we explored underlying mechanisms which DMC inhibited growth. Given ferrochelatase (FECH) is a potential target of identified thermal proteome profiling...
Dissection of the physiological interactomes histone post-translational modifications (hPTMs) is crucial for understanding epigenetic regulatory pathways. Peptide- or protein-based photoaffinity tools expanded ability to probe interactome, but in situ profiling native cells remains challenging. Here, we develop a nucleus-targeting histone-tail-based capable hPTM-mediated cells, by integrating cell-permeable and nuclear localization peptide modules into an hPTM equipped with photoreactive...
Vitis amurensis is a wild plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during acclimation (CA) in V. remains largely unknown. In this study, plantlets and vinifera cv. Muscat Hamburg were treated at 4 °C for 24 72 h, changes leaves detected by gas chromatography coupled with time-of-flight mass spectrometry. Most identified metabolites, including carbohydrates, amino acids, organic accumulated two types grape after CA. Galactinol, raffinose,...
Many encapsulated bacteria use capsules to cause invasive diseases. However, it remains largely unknown how the enhance bacterial virulence under in vivo infection conditions. Here we show that primarily target liver survival at onset of blood-borne infections. In a mouse sepsis model, enabled human pathogens Streptococcus pneumoniae and Escherichia coli circumvent recognition liver-resident macrophage Kupffer cells (KCs) capsular serotype-dependent manner. contrast effective capture...
Abstract Bio‐based epoxy resins with low‐dielectric constant, low viscosity and excellent mechanical properties have great significance for the future of advanced electronic microelectronic industries. Cyclosiloxane segment possesses polarity, high‐dissociation energy, elevated molecular volume, thereby making it useful synthesis materials dielectric constant viscosity. Herein, a tetrafunctional eugenol‐based resin (DEP) based on cyclosiloxane structure was designed prepared through...
Photocaged, activity-based ubiquitin (Ub) probes (Ub-ABPs) have been developed for the time-resolved probing of deubiquitinating enzyme (DUB) activities, but many Ub-ABPs are still challenging to photocage because their warheads (e.g. propargylamide (PA) or dehydroalanine (Dha)) difficult temporally block and activate. Here, we describe a new C-terminal backbone modification strategy construction photocaged in which light-sensitive group is placed at amide bond Ub Gly75. This enabled facile...
We report the first cell-penetrating Ub-Dha probes based on thiazolidine-protected cysteines, which were successfully used to label and enrich E1–E2–E3 enzymes in live cells combination with label-free quantification (LFQ) mass spectrometry.
Microglia, as resident immune cells in the central nervous system (CNS), play a crucial role maintaining homeostasis and phagocytosing metabolic waste brain. Senescent microglia exhibit decreased phagocytic capacity increased neuroinflammation through senescence-associated secretory phenotype (SASP). This process contributes to development of various neurodegenerative diseases, including Alzheimer's disease (AD). In this study, we found that SASP was elevated senescent microglia, proteomics...
Bromodomains, epigenetic "readers" of lysine acetylation marks, exist in different nuclear proteins with diverse biological functions chromatin biology. Malfunctions bromodomains are associated the pathogenesis human diseases, such as cancer. Bromodomains have therefore emerged therapeutic targets for drug discovery. Given high structural similarity bromodomains, a critical step development bromodomain inhibitors is evaluation their selectivity to avoid off-target effects. While numerous...
Abstract The tripartite motif-containing protein 21 (TRIM21) plays important roles in autophagy and innate immunity. Here, we found that HECT RLD domain containing E3 ubiquitin ligase 5 (HERC5), as an interferon-stimulated gene 15 (ISG15) ligase, catalyzes the ISGylation of TRIM21 at Lys260 Lys279 residues. Moreover, IFN- β also induces multiple lysine residues, thereby enhancing its activity for K63-linkage-specific ubiquitination resulting increased levels p62 K63-linked ubiquitination....
Interactions among metabolic pathways are important in plant biology. At present, not much is known about how folate metabolism affects other plants. Here we report a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB) was defective seed reserves and skotomorphogenesis. Lower carbon (C) higher nitrogen (N) content seeds than that wild type were indicative an altered C N partitioning capacity. Higher levels organic acids sugars detected compared with...