A5031816004- Escherichia coli research studies
- Vector-borne infectious diseases
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Pneumonia and Respiratory Infections
- Toxin Mechanisms and Immunotoxins
- Legume Nitrogen Fixing Symbiosis
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Plant-Microbe Interactions and Immunity
- Vibrio bacteria research studies
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Yersinia bacterium, plague, ectoparasites research
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Streptococcal Infections and Treatments
- Inflammasome and immune disorders
- Plant Pathogenic Bacteria Studies
- Bacterial Infections and Vaccines
- Plant Micronutrient Interactions and Effects
- Antibiotic Resistance in Bacteria
- Insect symbiosis and bacterial influences
- Iron Metabolism and Disorders
Tufts University
2016-2025
Tufts Medical Center
1992-2021
University of Massachusetts Chan Medical School
2006-2015
Jacksonville University
2014
Laboratory of Molecular Genetics
2001-2005
National Institute of Allergy and Infectious Diseases
2003
Massachusetts Institute of Technology
2003
Center for Cancer Research
2003
Chinese University of Hong Kong
2003
University of Hong Kong
2003
Specific strains of the Pseudomonas fluorescens-Pseudomonas putida group rapidly colonize plant roots several crops and cause statistically significant yield increases (38). Enhanced growth caused by these is often accompanied reductions in root-zone populations fungi bacteria. These beneficial are also effective biocontrol agents certain soil borne fungal pathogens This article reviews describes recent work implicating siderophores [microbial iron(III)-transport agents] produced...
Chronic Lyme arthritis that is unresponsive to antibiotic therapy associated with an increased frequency of the HLA-DR4 specificity. To determine whether immune response a particular polypeptide Borrelia burgdorferi may be treatment-resistant chronic arthritis, we correlated clinical courses and HLA-DR specificities 128 patients disease their antibody responses spirochetal polypeptides. Antibody reactivity was determined by Western blotting (immunoblotting) sonicated whole B. recombinant...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of ferric pseudobactin: a siderophore from plant growth promoting PseudomonasMartin Teintze, M. B. Hossain, C. L. Barnes, John Leong, and Dick Van der HelmCite this: Biochemistry 1981, 20, 22, 6446–6457Publication Date (Print):October 1, 1981Publication History Published online1 May 2002Published inissue 1 October 1981https://pubs.acs.org/doi/10.1021/bi00525a025https://doi.org/10.1021/bi00525a025research-articleACS PublicationsRequest...
establishment of lysogeny involves integration the circular X genome into bacterial chromosome by a sitespecific recombination event involving phage attachment site (designated attP or POP') and attB BOB').DNA sequence analyses ' A. Siege1 R. J. Deans, unpublished data.
Borrelia burgdorferi (Bb) is the causative agent of Lyme disease in United States, a that can result carditis, and chronic debilitating arthritis and/or neurologic symptoms if left untreated. Bb survives midgut Ixodes scapularis tick, or within tissues immunocompetent hosts. In early stages infection, bacteria are present bloodstream where they must resist clearance by innate immune system host. We have found novel role for outer surface protein C (OspC) from B. garinii interactions with...
Significance The nucleotide-binding domain and leucine rich repeat containing family, pyrin 3 (NLRP3) inflammasome regulates capase-1-dependent maturation of interleukin-1β during infection with Gram-negative bacterial pathogens such as enterohemorrhagic Escherichia coli . Here we identified RNA:DNA hybrids well RNA critical mediators these responses. gained access to the cytosol from phagolysosomal compartments infection, leading assembly NLRP3 complex. Delivery synthetic into triggered...
Significance The elderly exhibit susceptibility to many infectious agents, including S. pneumoniae . A robust acute inflammatory response is associated with severe disease. triggers canonical autophagy in nonprofessional phagocytes, but its role macrophages largely unexplored. We found that BMDMs utilize LAP, rather than autophagy, both eliminate and modulate inflammation. Notably, compared young BMDMs, aged were deficient resulting compromised bacterial killing enhanced proinflammatory...
Lyme disease is the most common vector-borne in North America and Europe. The clinical manifestations of vary based on genospecies infecting Borrelia burgdorferi spirochete, but microbial genetic elements underlying these associations are not known. Here, we report whole genome sequence (WGS) analysis 299 B . ( Bb ) isolates derived from patients Eastern Midwestern US Central We develop a WGS-based classification isolates, confirm extend findings previous single- multi-locus typing systems,...
Lyme disease is a chronic, multisystemic infection caused by the tick-borne spirochete Borrelia burgdorferi. Attachment of to host cells via specific receptors likely be important in establishment infection. B. burgdorferi have previously been shown bind variety mammalian vitro. Here we demonstrate that binding human platelets mediated integrin alpha IIb beta 3 (glycoprotein IIb-IIIa), critical receptor thrombosis and hemostasis. Functional expression this requires platelet activation, was...
Summary Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. (EHEC) each promote the reorganization of actin into filamentous pedestal structures beneath attached bacteria during colonization intestinal epithelium. Central to this process is translocation protein Tir (translocated intimin receptor) plasma membrane host cells, where it interacts with bacterial outer triggers cellular signalling events that lead rearrangement. Actin by EPEC requires a tyrosine residue, Y474,...
Abstract The rate of pathogen clearance is a critical determinant morbidity and mortality. We sought to characterize the immune response responsible for remarkably rapid individual episodes bacteremia caused by relapsing fever bacterium, Borrelia hermsii. SCID or Rag−/− mice were incapable resolving B. hermsii infection, indicating role T and/or B cells. TCR−/− mice, which lack cells, IL-7−/− are deficient in both cells follicular but not B1 splenic marginal zone (MZ) efficiently cleared...
Borrelia burgdorferi, the agent of Lyme disease, causes a multisystemic illness that can affect skin, heart, joints, and nervous system is capable attachment to diverse cell types. Among host components recognized by this spirochete are fibronectin glycosaminoglycans (GAGs). Three surface-localized GAG-binding bacterial ligands, Bgp, DbpA, DbpB, have been previously identified, but recent studies suggested at least one additional ligand expressed on spirochetal surface when adapted mammalian...
Binding of glycosaminoglycans (GAGs) by Borrelia burgdorferi , the Lyme disease spirochete, has potential to promote colonization diverse tissues. GAG binding B. is associated with haemagglutination and we have identified a 26 kDa protein, which termed Bgp ( B orrelia G AG‐binding p rotein), on basis its ability bind heparin erythrocytes. was found in outer membrane fractions surface intact bacteria, as assayed labelling membrane‐impermeable biotinylating agent or anti‐Bgp antibodies....
The spirochaetal agents of Lyme disease, Borrelia burgdorferi ( sensu lato ) bind to integrins α IIb β 3 , v and 5 1 in purified form on the surfaces human cells. Using a phage display library B. stricto DNA, candidate ligand for ‐chain was identified. native protein, termed p66, is known be recognized by disease patient sera expressed surface spirochaete. We show here that recombinant p66 binds specifically inhibits attachment intact same integrins. When Escherichia coli this protein...