A5077133720- Botulinum Toxin and Related Neurological Disorders
- Monoclonal and Polyclonal Antibodies Research
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Neurological disorders and treatments
- Toxin Mechanisms and Immunotoxins
- Hereditary Neurological Disorders
- Trace Elements in Health
- Helminth infection and control
- Biochemical and Structural Characterization
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Research on Leishmaniasis Studies
- RNA Interference and Gene Delivery
- Biochemical and Molecular Research
- Parasitic Infections and Diagnostics
- Neuropeptides and Animal Physiology
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Entomopathogenic Microorganisms in Pest Control
Tufts University
2015-2025
New England Disabled Sports
2013-2024
AgResearch
1999-2015
National Institutes of Health
1984-2015
National Institute of Allergy and Infectious Diseases
2015
Synaptic Research (United States)
2013
Regional Medical Center
2008
Harvard University
1991-2001
Cairo University
2001
University of Massachusetts Chan Medical School
1999
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means a mammalian cell (monkey COS cell) expression screening system. One these clones was used to produce recombinant GM-CSF in cells. The hematopoietin similar natural product that purified apparent homogeneity from medium conditioned T-cell line. found be 60 percent homologous with recently cloned murine lung messenger RNA.
Piercing Botulinum's Defense Botulinum neurotoxins (BoNTs) are poisons that cause muscle paralysis. In the acidic intestine, ingested BoNTs protected within a progenitor toxin complex. The toxins released on absorption into neutral bloodstream. Gu et al. (p. 977 ; see Perspective by Adler ) present structure of minimally functional complex—BoNT in complex with nontoxic, nonhemagglutinin protein at 2.7 angstrom resolution. structure, together biochemical studies, showed how assembly is...
Closed circular Moloney murine leukemia virus (M-MuLV) DNA was prepared from recently infected cells and cloned in a lambda vector. Four classes of M-MuLV inserts were found: Class I, full length 8.8-kilobase (kb) with two tandem long terminal repeats (LTRs) 600 base pairs; class 2, 8.2-kb single copy LTR; 3, various portions deleted; 4, an 8.8-kb insert internal sequence inversion. Determination nucleotide at the junction between LTRs 1 suggested that circularization occurred by blunt-end...
The N-terminal region of the gene encoding polyhedrin, major occlusion protein insect baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV), has been fused to DNA Escherichia coli beta-galactosidase. was inserted into AcNPV genome by cotransfection cells with recombinant plasmid and wild-type genomic DNA. Recombinant viruses were selected as blue plaques in presence a beta-galactosidase indicator, 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside. Studies one such virus,...
Factor IX has been expressed to high levels within a recombinant host cell and the biologically active fraction subsequently purified homogeneity for characterization.The coding sequence was inserted into mammalian expression vector transfected dihydrofolate reductase-deficient Chinese hamster ovary cells.The integrated DNA amplified copy number by selection increasingly higher of marker gene, reductase, contained co-transfected plasmid.Cloned lines secreting over 100 bg/ ml antigen up 1.5...
Abstract Bloodfeeding arthropods transmit many of the world’s most serious infectious diseases. Leishmania are transmitted to their mammalian hosts when an infected sandfly probes in skin for a bloodmeal and injects parasite mixed with its saliva. Arthropod saliva contains molecules that affect blood flow modulate immune response host. Indeed, markedly enhances infectivity L. major If salivary molecule(s) responsible this phenomenon was identified, it might be possible vaccinate host against...
The gene for murine erythropoietin (EPO) was isolated from a mouse genomic library with human EPO cDNA probe. Nucleotide sequence analysis permitted the identification of coding and prediction encoded amino acid based on conservation between genes. Both DNA sequences were 80% conserved two species. Transformation COS-1 cells mammalian cell expression vector containing region resulted in secretion biological activity both erythroid progenitor cells. transcription start site kidneys...
COMMERCIAL concentrates of human antihemophilic factor (factor VIII) have been available for nearly 20 years and resulted in dramatic changes the treatment classic hemophilia. Home therapy, surgical intervention, prolonged hospitalized patients with hemophilia all facilitated by availability lyophilized VIII preparations.Although improved ease effectiveness treatment, transfusion-associated complications also increased. Patients a high frequency seropositivity immunodeficiency virus type 1...
Research Article9 August 2017Open Access Source DataTransparent process mRNA mediates passive vaccination against infectious agents, toxins, and tumors Moritz Thran CureVac AG, Tübingen, Germany Search for more papers by this author Jean Mukherjee Department of Infectious Disease Global Health, Tufts Cummings School Veterinary Medicine, North Grafton, MA, USA Marion Pönisch Katja Fiedler Andreas Thess Barbara L Mui Acuitas Therapeutics, Vancouver, BC, Canada Michael J Hope Ying K Tam Nigel...
The incidence of Clostridium difficile infection (CDI) and associated mortality have increased rapidly worldwide in recent years. Therefore, it is critical to develop new therapies for CDI. In this study, we generated a novel, potently neutralizing, tetravalent, bispecific antibody composed 2 heavy-chain-only VH (VHH) binding domains against both TcdA TcdB (designated "ABA") that reverses fulminant CDI mice infected with an epidemic 027 strain after single injection the antibody. We...
Abstract We report here the results of studies showing that inhibition C is a property several invertebrate paramyosins. Paramyosins from Taenia solium, Schistosoma mansoni, and mussel Mytilus edulis bind polymeric collagen can be isolated crude extracts tissues by affinity. These paramyosins inhibit C1 function whether or present in C2-deficient serum. Because T. solium paramyosin was best inhibitor, we concentrated further on this molecule. binds purified C1q solution with dose/response...
Calcitonin gene-related peptide (CGRP), a potent vasodilatory present in central and peripheral neurons, is released at inflammatory sites inhibits several macrophage, dendritic cell, lymphocyte functions. In the study, we investigated role of CGRP models local systemic acute inflammation on macrophage activation induced by lipopolysaccharide (LPS). Intraperitoneal pretreatment with synthetic reduces approximately 50% number neutrophils blood into peritoneal cavity 4 h after LPS injection....
By screening a library of unintegrated, circular Moloney murine leukemia virus (M-MuLV) DNA cloned in lambda phage, we found that approximately 20% the M-MuLV inserts contained internal sequence deletions or inversions. Restriction enzyme mapping demonstrated tht deleted segments frequently abutted long terminal repeat (LTR) sequence, whereas inverted were usually flanked by LTR sequences, suggesting many variants arose as consequence molecules integrating within their own DNA. Nucleotide...
Shiga toxin-producing Escherichia coli (STEC) is a major cause of severe food-borne disease worldwide, and two toxins, Stx1 Stx2, are primarily responsible for the serious consequence, hemolytic-uremic syndrome (HUS). Here we report identification panel heavy-chain-only antibody (Ab) V(H) (VHH) domains that neutralize and/or Stx2 in cell-based assays. VHH heterodimer toxin-neutralizing agents containing linked Stx1-neutralizing VHHs or Stx2-neutralizing were generally much more potent at Stx...
Adult schistosomes live in the host's bloodstream where they import nutrients such as glucose across their body surface (the tegument). The parasite tegument is an unusual structure since it enclosed not by typical one but two closely apposed lipid bilayers. Within importing proteins have been identified; these are schistosome transporter (SGTP) 1 and 4. SGTP4 present host interactive, apical tegumental membranes, while SGTP1 found basal membrane (as well internal tissues). SGTPs act...
Botulinum neurotoxins (BoNTs) are the most potent natural toxins known. The effects of BoNT serotype A (BoNT/A) can last several months, whereas E (BoNT/E), which shares same synaptic target, synaptosomal-associated protein 25 (SNAP25), only weeks. long-lasting or persistence BoNT/A, although desirable for therapeutic applications, presents a challenge medical treatment intoxication. Although mechanisms toxicity well known, little is known about that govern toxins. We show recombinant...