A5018941611- Virus-based gene therapy research
- Metabolism and Genetic Disorders
- Biochemical and Molecular Research
- Bipolar Disorder and Treatment
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Virology and Viral Diseases
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Dialysis and Renal Disease Management
- RNA Research and Splicing
- Animal Virus Infections Studies
- Enzyme Structure and Function
- Pluripotent Stem Cells Research
- Photosynthetic Processes and Mechanisms
- Synthesis and Reactions of Organic Compounds
- Vector-Borne Animal Diseases
- interferon and immune responses
Sumitomo Dainippon Pharma (Japan)
1995-2025
Ōtani University
2024
Kokusai Junior College
2024
Niigata University
2023-2024
Sumitomo Dainippon Pharma (United States)
2024
Inoue Hospital
2016-2024
Tokushima University
2023
Aichi Gakuin University
2022
Dai Nippon Printing (Japan)
2021
Life Science Institute
2021
After the first report of induced pluripotent stem cells (iPSCs), considerable efforts have been made to develop more efficient methods for generating iPSCs without foreign gene insertions. Here we show that Sendai virus vector, an RNA vector carries no risk integrating into host genome, is a practical solution generation safer iPSCs. We improved vectors by introducing temperature-sensitive mutations so could be easily removed at nonpermissive temperatures. Using these enabled production...
Gallic acid (3,4,5-trihydroxybenzoic acid) is a naturally occurring plant phenol obtained by the hydrolysis of tannins and know to show some pharmacological activities. In screening anti-cancer agents in traditional Chinese medicines, gallic was found cytotoxicity against all cancer cells that we examined this study (IC50s: 4.8-13.2 micrograms/ml). primary cultured rat hepatocytes macrophages, lesser fibroblasts endothelial cells. Cell death dRLh-84 occurred within 6h after treatment at...
The various polyunsaturated fatty acids found in cellular lipids are transferred from their coenzyme A thiol esters to phospholipid acceptors with greatly different maximal velocities.The very low apparent K , values for the acylating 1-acylglycerol-3-phosphocholine could not be directly measured, but estimated relative that arachidonate.The competitive effectiveness of acyl-CoAs was by measuring equivalent concentrations which allow incorporations equal arachidonoyl-CoA.These help predict...
The WRKY proteins comprise a major family of transcription factors that are essential in pathogen and salicylic acid responses higher plants as well variety plant-specific reactions. They share DNA binding domain, designated the which contains an invariant WRKYGQK sequence CX4-5CX22-23HXH zinc motif. Herein, we report NMR solution structure C-terminal domain Arabidopsis thaliana WRKY4 protein. consists four-stranded beta-sheet, with pocket formed by conserved Cys/His residues located at one...
We have recently shown that non-viral gene therapy can stabilise the decline of lung function in patients with cystic fibrosis (CF). However, effect was modest, and more potent transfer agents are still required. Fuson protein (F)/Hemagglutinin/Neuraminidase (HN)-pseudotyped lentiviral vectors efficient for than preclinical models. In preparation a first-in-man CF trial using vector, we undertaken key translational studies. Regulatory-compliant carrying range promoter/enhancer elements were...
Abstract Excessive production of airway mucus is a cardinal feature bronchial asthma and chronic obstructive pulmonary disease (COPD) contributes to morbidity mortality in these diseases. IL-13, Th2-type cytokine, central mediator the pathogenesis asthma, including overproduction. Using genome-wide search for genes induced epithelial cells response we identified pendrin encoded by SLC26A4 (PDS) gene as molecule responsible production. In both COPD mouse models, was up-regulated at apical...
Abstract The B3 DNA binding domain is shared amongst various plant-specific transcription factors, including factors involved in auxin-regulated and abscisic acid–regulated transcription. Herein, we report the NMR solution structure of Arabidopsis thaliana cold-responsive factor RAV1. consists a seven-stranded open β-barrel two α-helices located at ends barrel significantly similar to noncatalytic restriction enzyme EcoRII. An titration experiment revealed recognition interface that enabled...
Gene therapy for cystic fibrosis (CF) is making encouraging progress into clinical trials. However, further improvements in transduction efficiency are desired. To develop a novel gene transfer vector that improved and truly effective CF therapy, simian immunodeficiency virus (SIV) was pseudotyped with envelope proteins from Sendai (SeV), which known to efficiently transduce unconditioned airway epithelial cells the apical side. This evaluated mice vivo vitro directed toward therapy. Here,...
The WRKY family transcription factors regulate plant-specific reactions that are mostly related to biotic and abiotic stresses. They share the domain, which recognizes a DNA element (TTGAC(C/T)) termed W-box, in target genes. Here, we determined solution structure of C-terminal domain Arabidopsis WRKY4 complex with W-box by NMR. A four-stranded β-sheet enters major groove an atypical mode β-wedge, where sheet is nearly perpendicular helical axis. Residues conserved WRKYGQK motif contact...
Rationale: Ongoing efforts to improve pulmonary gene transfer thereby enabling therapy for the treatment of lung diseases, such as cystic fibrosis (CF), has led assessment a lentiviral vector (simian immunodeficiency virus [SIV]) pseudotyped with Sendai envelope proteins F and HN.Objectives: To place this onto translational pathway clinic by addressing some key milestones that have be achieved.Methods: F/HN-SIV transduction efficiency, duration expression, toxicity were assessed in mice. In...
We studied the protective effects of Echinacea purpurea against radiation by evaluating changes in peripheral blood cell count and antioxidant activity. E. administration had a suppressive effect on radiation-induced leukopenia, especially lymphocytes monocytes, resulted faster recovery counts. Mouse activity was increased purpurea, relationship between leukopenia suggested. Furthermore, we reviewed evidence augmentation found this study humoral immunity. The effecst immune activation were...
In addition to their pleiotropic functions under physiological conditions, transcription factors STAT3 and STAT5 also have oncogenic activities, but how activated STATs are transported the nucleus has not been fully understood. Here we show that an MgcRacGAP mutant lacking its nuclear localizing signal (NLS) blocks translocation of p-STATs both in vitro vivo. Unlike wild-type MgcRacGAP, this did promote complex formation phosphorylated (p-STATs) with importin alpha presence GTP-bound Rac1,...
Objectives: This study aimed to evaluate the safety of CT-P13 in patients with rheumatoid arthritis (RA) during long-term treatment or after switching from innovator infliximab (IFX). Methods: Patients who completed 54 weeks a phase I/II (PI/II) received at an initial dose 3 mg/kg Week 62, increases permitted up 10 mg/kg. The primary endpoint was adverse event (AE) incidence. Results: Thirty-four 38 maintenance group and 29 33 switch reported least one AE. Safety profiles both groups were...