A5065310458- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- Cytokine Signaling Pathways and Interactions
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Pharmacogenetics and Drug Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Hormonal Regulation and Hypertension
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Phytoestrogen effects and research
- Cancer, Lipids, and Metabolism
- Genomics and Chromatin Dynamics
- Drug Transport and Resistance Mechanisms
- Hormonal and reproductive studies
- Computational Drug Discovery Methods
- RNA Research and Splicing
- Inflammatory mediators and NSAID effects
- Pancreatic and Hepatic Oncology Research
- FOXO transcription factor regulation
- Colorectal Cancer Treatments and Studies
- Pancreatic function and diabetes
- Cell death mechanisms and regulation
Amager Hospital
2025
Hvidovre Hospital
2025
Copenhagen University Hospital
2025
Karolinska Institutet
2000-2023
University of Houston
2013-2023
Memorial Sloan Kettering Cancer Center
2015
Molecular Oncology (United States)
2015
Receptors (United States)
2013
Karolinska University Hospital
1992-2009
University of Tennessee Medical Center
2004
Estrogen receptor (ER) β counteracts the activity of ERα in many systems. In agreement with this, we show this study that induced expression ERβ breast cancer cell line T47D reduces 17β-estradiol-stimulated proliferation when mRNA equals ERα. Induction growth exponentially proliferating cells a concomitant decrease components cycle associated proliferation, namely cyclin E, Cdc25A (a key regulator Cdk2), p45 Skp2 p27 Kip1 proteolysis), and an increase Cdk inhibitor . We also observed reduced...
Abstract A hallmark of pancreatic ductal adenocarcinoma (PDAC) is an exuberant stroma comprised diverse cell types that enable or suppress tumor progression. Here, we explored the role oncogenic KRAS in protumorigenic signaling interactions between cancer cells and host cells. We show mutation (KRAS*) drives cell-autonomous expression type I cytokine receptor complexes (IL2rγ–IL4rα IL2rγ–IL13rα1) turn are capable receiving growth signals (IL4 IL13) provided by invading Th2 microenvironment....
Abstract Estrogens, which are stimulators of growth both the normal breast and malignant breast, mediate their effects through two estrogen receptors (ER), namely ERα ERβ. mediates proliferative effect in cancer cells, whereas ERβ seems to be antiproliferative. We engineered ERα-positive T47D cells express a Tet-Off–regulated manner. These were then injected orthotopically into severe combined immunodeficient mice, resulting tumors was compared with from injecting parental that do not The...
Abstract Estrogen receptor β (ERβ) is the predominant ER in colorectal epithelium. Compared with normal colon tissue, ERβ expression reduced cancer. Our hypothesis that inhibits proliferation of cancer cells. Hence, aim this study has been to investigate molecular function cells, focusing on cell cycle regulation. SW480 cells have lentivirus transduced construct or without mutated DNA-binding domain an empty control vector. Expression resulted inhibition and G1 phase arrest effect was...
In this study, an estrogen receptor (ER) α-expressing T47D cell line containing inducible tet-off FLAG-ERβ was used to examine the influence of ERβ on ERα activity. Real-time PCR analysis mRNA levels two well-studied estrogen-responsive genes, pS2 and progesterone (PR), showed that expression both genes were reduced in presence ERβ. Chromatin immunoprecipitation assays 17β-estradiol (E2)-induced recruitment patterns PR promoters similar for did not significantly kinetic profile promoter, but...
The onset of the sexually dimorphic pattern GH secretion and increased hepatic GH-binding capacity in rats at puberty is temporally correlated with developmental induction three cytochrome P-450s steroid hydroxylase activity, P-450 IIC11, IIC12, IIC13, one vitamin A IIC7. In this study we demonstrate that expression 2C11, 2C12, 2C13 genes modulated by level transcriptional initiation both vivo primary cultures adult hepatocytes. an effort to define minimum sequence responsible for inductive...
Abstract Estrogens, by binding to and activating two estrogen receptors (ERα ERβ), are critically involved in the development of mammary gland breast cancer. An isoform ERβ, ERβ2 (also called ERβcx), with an altered COOH-terminal region, is coexpressed ERα many human cancers. In this study, we generated a stable cell line from MCF7 cancer cells expressing inducible version ERβ2, along endogenous ERα, examined effects on protein levels function. We showed that inhibited ERα-mediated...
Breast cancer cells show overexpression of estrogen receptor (ER) α relative to ERβ compared normal breast tissues. This observation has lead the hypothesis that may modulate proliferative effect ERα. study investigated how variable cellular expression ratios ERα and effects on cell proliferation induced by or agonists, respectively. Using human osteosarcoma (U2OS) reporter cells, propyl-pyrazole-triol (PPT) was shown be a selective diarylpropionitrile (DPN) preferential modulator. The these...
Several studies suggest estrogen to be protective against the development of colon cancer. Estrogen receptor β (ERβ) is predominant expressed in colorectal epithelium and main candidate mediate effects. We have previously shown that expression ERβ reduces growth cancer xenografts. Little known actions its effect on gene transcription cancers. To dissect processes mediates investigate cell-specific mechanisms, we reexpressed three cell lines (SW480, HT29, HCT-116) conducted genome-wide...
In this article, we have applied the ChIP-on-chip approach to pursue a large scale identification of ERalpha- and ERbeta-binding DNA regions in intact chromatin. We show that there is high degree overlap between identified as bound by ERalpha ERbeta, respectively, but are also only presence well selectively either receptor. Analysis shows distinct properties terms genome landscape, sequence features, conservation compared with ERbeta. ERbeta-bound are, group, located more closely...
The estrogen receptor (ER)β1 is successively lost during cancer progression, whereas its splice variant, ERβ2, expressed in advanced prostate cancer. latter form of often metastasizes to bone, and we wanted investigate whether the loss ERβ1 and/or expression ERβ2 affect such signaling pathways Using PC3 22Rv1 cell lines that stably express or found ERβ variants differentially regulate genes known tumor behavior. We repressed bone metastasis regulator Runx2 cells. By contrast, RUNX2 was...
Abstract Introduction The impact of interactions between the two estrogen receptor (ER) subtypes, ERα and ERβ, on gene expression in breast cancer biology is not clear. goal this study was to examine transcriptomic alterations cells co-expressing both receptors association signatures with disease outcome. Methods Transcriptional effects ERβ overexpression were determined a stably transfected cell line derived from ERα-positive T-47D cells. Microarray analysis carried out identify...
Abstract Glioblastomas (GBM), deadly brain tumors, have greater incidence in males than females. Epidemiological evidence supports a tumor suppressive role of estrogen; however, estrogen as potential therapy for GBM is limited due to safety concerns. Since express ERβ, second receptor estrogen, targeting ERβ with selective agonist may be novel therapy. In the present study, we examined therapeutic effect synthetic LY500307 using vitro and vivo models. Treatment significantly reduced...
The induction of neurite outgrowth by NGF is a transcription-dependent process in PC12 cells, but the transcription factors that mediate this are not known. Here we show bHLH transcriptional repressor HES-1 mediator process. Inactivation endogenous forced expression dominant-negative protein induces absence and increases response to NGF. In contrast, additional wild-type represses delays Endogenous DNA-binding activity post-translationally inhibited during signaling vivo, phosphorylation PKC...
Hepatic expression of various members the cytochrome P-450 (CYP) superfamily is suppressed during inflammatory responses. We have shown that specific 2C11 in male rat liver transcriptionally by endotoxin treatment. To investigate molecular mechanisms underlying this phenomenon, we studied effects cytokines interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF), interferon (IFN)-alpha, and IFN-gamma on mRNAs two typical acute-phase protein genes, alpha 1-acid glycoprotein (AGP)...
The expression of human small nuclear U2 RNA genes is controlled by the proximal sequence element (PSE), which determines start site transcription, and a distal (DSE). DSE contains an octamer three Sp1 binding sites. octamer, like PSE, essential for transcription. sites contribute to full promoter activity distance-dependent cooperative interactions with transcription factors Oct-1. Here we show that purified recombinant Oct-1 bind cooperatively they physically interact in vitro ....
Abstract Estrogen effects on mammary gland development and differentiation are mediated by two receptors (ERα ERβ). Estrogen‐bound ERα induces proliferation of epithelial cancer cells, while ERβ is important for maintenance the differentiated epithelium inhibits in different cell systems. In addition, normal breast contains higher levels compared to early stage cancers, suggesting that loss could be development. Analysis ERβ−/− mice has consistently revealed reduced expression adhesion...