A5006390201- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- Wnt/β-catenin signaling in development and cancer
- Congenital heart defects research
- Plant Molecular Biology Research
- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Plant nutrient uptake and metabolism
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Plant Reproductive Biology
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Connective tissue disorders research
- Neurogenetic and Muscular Disorders Research
- Bioinformatics and Genomic Networks
- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Date Palm Research Studies
- Lipid metabolism and biosynthesis
CSIRO Health and Biosecurity
2023-2024
Commonwealth Scientific and Industrial Research Organisation
2024
Australian Regenerative Medicine Institute
2011-2022
Monash University
2011-2022
EMBL Australia
2011-2019
Centre National de la Recherche Scientifique
1997-2009
Institut de Biologie du Développement Marseille
2007-2009
Utrecht University
2002-2007
Universidade Federal do Rio de Janeiro
2002
Although the genetic triggers for gonadal sex differentiation vary across species, cell biology of development was long thought to be largely conserved. Here, we present a comprehensive analysis differentiation, using single-cell sequencing in embryonic chicken gonad during sexual differentiation. The data show that embryonic-supporting cells do not derive from coelomic epithelium, contrast other vertebrates studied. Instead, they DMRT1+/PAX2+/WNT4+/OSR1+ mesenchymal population. We find...
In plant meristems, dividing cells interpret positional information and translate it into patterned cell differentiation. Here we report the molecular identification of Arabidopsis HOBBIT gene that is required for division differentiation in meristems. We show encodes a homolog CDC27 subunit anaphase-promoting complex (APC). partially complements yeast nuc2/cdc27 mutant. Unlike other homologs , its transcription cycle regulated. Furthermore, hobbit mutants reduction DR5 :: GUS auxin reporter...
To investigate the specialization of two Arabidopsis CDC27 subunits in anaphase-promoting complex (APC/C), we analyzed novel alleles HBT/CDC27B and CDC27A, characterized expression complementing HOBBIT (HBT) protein fusions plant meristems during cell cycle. In contrast to other APC/C mutants, which are gametophytic lethal, phenotypes weak null hbt indicate a primary role control post-embryonic division elongation, whereas cdc27a nulls phenotypically indistinguishable from wild type....
The Arabidopsis HOBBIT ( HBT ) gene encodes a homolog of the CDC27 anaphase-promoting complex/cyclosome subunit and is essential for postembryonic development. We induced loss-of-function clones by Cre/lox -mediated recombination single complementing transgene in background homozygous strong mutant allele hbt 2311 . Defects cell division expansion are primary consequences ubiquitous excision. In roots, both rapidly affected. contrast, leaf primordia, halt after lag phase, which results...
In amniotes, it is widely accepted that WNTs secreted by the dorsal neural tube form a concentration gradient regulates early somite patterning and myotome organization. Here we demonstrate in chicken embryo WNT protein not to act at distance, but rather loaded onto migrating crest cells deliver somites. Inhibiting migration or ablating their population has profound impact on response Furthermore, show central player efficient delivery of somites heparan sulfate proteoglycan GPC4, expressed...
Due to its amenability manipulations, live observation and striking similarities mammals, the chicken embryo has been one of major animal models in biomedical research. Although it is technically possible genome-edit chicken, long generation time (6 months sexual maturity) makes an impractical lab model prevented widespread use The Japanese quail (Coturnix coturnix japonica) attractive alternative, very similar but with decisive asset a much shorter (1.5 months). In recent years, transgenic...
In amniotes, it is widely accepted that WNTs secreted by the dorsal neural tube form a concentration gradient regulates early somite patterning and myotome organization.Here we demonstrate in chicken embryo WNT protein not to act at distance, but rather loaded onto migrating crest cells deliver somites.Inhibiting migration or ablating their population has profound impact on response somites.Furthermore, show central player efficient delivery of somites heparan sulfate proteoglycan GPC4,...
Here, we report the generation of a transgenic Lifeact–EGFP quail line for investigation actin organization and dynamics during morphogenesis in vivo. This avian allows high-resolution visualization structures within living embryo, from subcellular filaments that guide cell shape to supracellular assemblies coordinate movements across tissues. The unique suitability embryos live imaging facilitates previously intractable processes embryogenesis. Using approaches, present dynamic behaviors...
The use of shRNAmir to down‐regulate the expression genes interest is a powerful tool for studying gene function during early chick development. However, because limitations electroporation‐mediated transgenesis, down‐regulation expressed at late stages development in specific tissues difficult perform. By combining electroporation doxycycline‐inducible, miR30‐based shRNA plasmid with Tol2 genomic integration system, we are now able any defined stage chicken genesis 51:372–380. © 2013 Wiley...
The Wnt/β-catenin signaling pathway is highly conserved throughout evolution, playing crucial roles in several developmental and pathological processes. Wnt ligands can act at a considerable distance from their sources it therefore necessary to examine not only the Wnt-producing but also Wnt-receiving cells tissues fully appreciate many functions of this pathway. To monitor activity, multiple tools have been designed which consist multimerized response elements (TCF/LEF binding sites)...
GIF-DB (Gene Interactions in the Fly Database) is a new WWW database (http://www-biol.univ-mrs.fr/ approximately lgpd/GIFTS_home_page. html ) describing gene molecular interactions involved process of embryonic pattern formation flyDrosophila melanogaster. The detailed information distributed specific lines arranged into an EMBL- (or SWISS-PROT-) like format. achieves high level integration with other databases such as FlyBase, EMBL and SWISS-PROT through numerous hyperlinks. original...
Abstract Here we report the generation of a transgenic Lifeact–EGFP quail line for investigation actin organization and dynamics during morphogenesis in vivo . This avian allows high-resolution visualization structures within living embryo, from subcellular filaments that guide cell shape to supracellular assemblies coordinate movements across tissues. The unique suitability embryos live imaging facilitates previously intractable processes embryogenesis. Using approaches, present dynamic...
Abstract During development, dynamic changes in tissue shapes known as morphogenesis result from the exquisite orchestration of signalling pathways, cell–cell interactions, cell divisions, and coordinated movements. How cells within embryos adopt a vast array fates such an ever-changing environment has fascinated scientists for generations, yet ability to observe characterize those rapid proven technically challenging higher vertebrates. The japanese quail ( Coturnix coturnix japonica ) is...